4,908 results

1. pWALIUM10-V5-TurboID-GW

   Plasmid

   #196285

   Purpose

   Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies

   Depositor

   Norbert Perrimon

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

   Plasmid

   #218866

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8f
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRL-ubi63E

   Plasmid

   #74280

   Purpose

   Renilla control plasmid driven by the ubiquitin 63E promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   ubiquitin 63E promoter

   Available since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-RhoA FLARE.sc Biosensor Q63L

   Plasmid

   #12151

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor Q63L (RHOA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   RhoA Q63L (dominant-positive mutation)

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUASp YFP Ras85D CA

   Plasmid

   #52247

   Purpose

   Drosophila expression of YFP-Ras85D constitutively active mutant

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q61L; CA mutation

   Promoter

   GAL4

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

6. p130Cas

   Plasmid

   #186131

   Purpose

   For expression of recombinant p130Cas in SF9 cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   BCAR1 (Bcar1 Rat)
   

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

   Plasmid

   #63169

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-FLAG"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUAST-yki:FLAG(3x)

   Plasmid

   #85622

   Purpose

   Express 3x Flagged yki in Drosophila.

   Depositor

   Kenneth Irvine

   Article

   Oh et al Development. 2008 Mar;135(6):1081-8. doi

   Insert

   yki (yki Fly)
   

   Use

   Tags

   3x FLAG

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFD1-dU6:1gRNA

   Plasmid

   #49408

   Purpose

   expression of gRNA under control of the Drosophila U6:1 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-1:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOmniBac zz TEV YBBR POT1

    Plasmid

    #185444

    Purpose

    Expresses human POT1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Insert

    POT1 (POT1 Human)
    

    Use

    Tags

    YBBR and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTriEx-NLyn-Venus-Zdk1

    Plasmid

    #81042

    Purpose

    mammalian expression of plasma-membrane anchored Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, NLyn, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHACK-QF2

    Plasmid

    #80274

    Purpose

    QF2 HACK backbone. Contains QF2-hsp70, but no gRNAs

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcUW51-gEgIL

    Plasmid

    #11622

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

    Insert

    HSV-1 glycoprotein E and HSV-1 glycoprotein I
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Dicistronic vector containing HSV-1 gE (KOS strai…

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pCRISPaint-sfGFP-3xP3-RFP

    Plasmid

    #127566

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sfGFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT-Hrp48-ADARcd-E488Q-V5

    Plasmid

    #139685

    Purpose

    Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Hrp48, ADAR (Adar Fly)
    

    Use

    Tags

    V5

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-mVenus-Zdk1

    Plasmid

    #81047

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHD-SPARC2-I-LexA::p65

    Plasmid

    #133563

    Purpose

    Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    20X UAS

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJFRC-nSyb-IVS-PhiC31

    Plasmid

    #133868

    Purpose

    Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    PhiC31 Recombinase (int )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTriEx-RhoA FLARE.sc Biosensor T19N

    Plasmid

    #12152

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor T19N (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA T19N (dominant-negative mutation)*

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUAST-attB-yki:V5-S111A, S168A, S250A

    Plasmid

    #85620

    Purpose

    Expresses V5 tagged triple mutant yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

    Insert

    yki-V5-S111A,S168,S250A
    

    Use

    Tags

    V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge