4,932 results
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Plasmid#138406PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertV2 tail-TEVcs(ENLYFQL)-LexAp65
ExpressionInsectAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>GAL80
Plasmid#104874PurposeHACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.DepositorInsertT2A-GAL80
UseCRISPRExpressionInsectAvailable SinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAct-Fok1:dCas9
Plasmid#62211PurposeExpresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
ExpressionInsectPromoteract5CAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218865PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP
Plasmid#127560PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertCas9-T2A-GFP
UseCRISPRExpressionInsectMutationEGFP-D102G see depositor comments belowAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393
Plasmid#216382PurposeBaculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11DepositorInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTags6xHis-3C-splitGFP11ExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393
Plasmid#216380PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags3C-splitGFP1to10opt-ETVV and HA signal sequence-F…ExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a
Plasmid#216385PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectMutationC93A/C95A/C131APromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hNorrin(33-133)-1D4_pAcGP67a
Plasmid#216384PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-GCP3
Plasmid#178069PurposeInsect cell expression of GCP3DepositorInsertGCP3 (TUBGCP3 Human)
ExpressionInsectAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-7xHalo7::CAAX
Plasmid#87647PurposeFor creating drosophila transgenics expressing UAS-7xHalo7::CAAXDepositorInsert7xHalo7::CAAX
ExpressionInsectAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-shibire (ts1)
Plasmid#24362DepositorInsertShibire (ts1) (shi Fly)
ExpressionInsectAvailable SinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pXN-FBLWLF-LexA
Plasmid#26266DepositorInsertLexA
UseDrosophila transgenesisExpressionBacterial and InsectAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1-C450A
Plasmid#22021PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
Tags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV domain contains the light-insensitive C450A m…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 Exons 1-3
Plasmid#91799PurposeDrosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNADepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKF257
Plasmid#52533Purposeexpresses I-SceI nuclease in DrosophilaDepositorAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-EGFP-3'UTR#5
Plasmid#176337PurposeThe synthetic 3'UTR#5 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoterDepositorInsert3'UTR#5
ExpressionInsectAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR
Plasmid#83343Purposefor Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK4-S
Plasmid#172425PurposeExpression of full-length, human GIRK4 with C-terminal StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-K2-L2
Plasmid#80684PurposeGateway Entry vector containing a temperature-sensitive mouse DHFRDepositorUseSynthetic BiologyTags3xHAExpressionBacterial, Insect, Mammalia…Mutationmouse DHFR with N-terminal UbiquitinAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFHMSP-LIC-C
Plasmid#26100PurposeDonor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expressionDepositorTypeEmpty backboneUseBaculovirus expressionTags8x His tag and MKFLVNVALVFMVVYISYIYAAA (Honeybee …ExpressionInsectPromoterpolyhedrinAvailable SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJFRC7-UAS-NPRR_dTK
Plasmid#133244PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertdrosophila tachykinin-fused codon-optimized GCaMP6s
ExpressionInsectPromoterhsp70Available SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-H6-FGB
Plasmid#49203PurposeFluorescent reporter for cGMPDepositorInsertH6-FGB
TagsHis tag + Other tagsExpressionBacterial, Insect, and Mamm…MutationNonePromoterCMVAvailable SinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAc-GFP-dSUMO
Plasmid#85696Purposeexpression of GFP-SUMO1 in Drosophila cellsDepositorAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His6-Mps1-WT
Plasmid#114063Purposeprotein productionDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXL-BACII-LoxP-3xPDsRed-LoxP
Plasmid#26852DepositorInsertLoxP-3xPDsRed-LoxP
ExpressionInsectAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBac(3xP3-gTc’v; Tc-hsp68:Cre)
Plasmid#119063PurposeExpression of Cre recombinase in Tribolium castaneumDepositorInsertspBacL
Tc-hsp68 Promoter
Cre recombinase
Tc-hsp68 3'UTR
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR
ExpressionInsectAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR L1-vas2-Cre-L4
Plasmid#62301PurposeCre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoterDepositorInsertCre recombinase under control of vasa promoter and terminator
TagsHA and SV40 NLSExpressionInsectPromotervasaAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(WT)
Plasmid#86561PurposeExpresses human full-length Akt1(WT)DepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCoofy29
Plasmid#44005DepositorTypeEmpty backboneTagsHis6-MBPExpressionInsectPromoterPPHAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac (GST-preSci site-CD45)
Plasmid#177889PurposeBaculo expression of GST tag fused to human CD45 ICDDepositorInsertCD45 (aa 598-1304 only) (PTPRC Human)
TagsTEV-GST-PreScission cut siteExpressionInsectMutationL652PPromoterPolyhedrinAvailable SinceAug. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
HS-H3-GFP
Plasmid#8698DepositorAvailable SinceAug. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
AAEL007097-Cas9
Plasmid#100705PurposeThe promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL007097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL007097 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Nluc-3xP3-RFP
Plasmid#127563PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.DepositorInsertNluc
UseCRISPRExpressionInsectAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Agam_695
Plasmid#176668PurposeExpression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221893PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertTAF15 (TAF15 Human)
Tags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1259-014_Jie_EWSR1_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221894PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertEWSR1 (EWSR1 Human)
Tags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab18
Plasmid#31740DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)
Plasmid#55223DepositorTypeEmpty backboneTagsSNAPf and TwinStrepIIExpressionInsectAvailable SinceJuly 9, 2014AvailabilityAcademic Institutions and Nonprofits only