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5,046 results

Showing: 1171 - 1200 of 5046 results

  1. His-ZZ-TEV-BICDN(1-400)-GFP

    Plasmid
    #111862
    Purpose
    C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.
    Depositor
    Andrew Carter
    Article
    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do
    Insert
    BICDN(1-400)-GFP (Bicd2 Mouse)
    Tags
    8xHis tag, GFP, and ZZ tag
    Expression
    Insect
    Mutation
    Codon optimized for Sf9 expression
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111862-plasmid-map-sequence-id-215382

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  5. DPYSL4 (DPYSL4A-c003)

    Plasmid
    #110263
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPYSL4 (DPYSL4 Human)
    Tags
    His6-ZBasic-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110263-plasmid-map-sequence-id-212341

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  13. pMT-HA-STX1

    Plasmid
    #89310
    Purpose
    Expresses HA tagged syntaxin 1 in Drosophila cells
    Depositor
    Andrew Peden
    Insert
    STX1 (Syx1A Fly)
    Tags
    HA tag
    Expression
    Insect
    Promoter
    P-MT (Metallothionein)
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89310-plasmid-map-sequence-id-190170

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  18. pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]

    Plasmid
    #86454
    Purpose
    Nuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86454-plasmid-map-sequence-id-170900

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  19. pFastBacDual with LFn + PrgJ

    Plasmid
    #84869
    Purpose
    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    PrgJ
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84869-plasmid-map-sequence-id-162908

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  25. CDK12

    Plasmid
    #73258
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CDK12 (CDK12 Human)
    Tags
    His-6-TEV
    Expression
    Insect
    Mutation
    pfam00069: Pkinase 727-1020
    Promoter
    Polyhedrin
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pUAST-HA, Pbl-A

    Plasmid
    #69792
    Purpose
    Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    van Impel et al Development. 2009 Mar;136(5):813-22. doi
    Insert
    Pebble - Drosophila guanine nucleotide exchange factor
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA tag
    Expression
    Insect
    Mutation
    Encodes Pbl (NP_729306.1) lacking last 9 amino ac…
    Promoter
    hsp70 promoter
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1171 - 1200 of 5046 results