4,908 results

1. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217515

   Purpose

   Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQUAST-attB

   Plasmid

   #104880

   Purpose

   For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system

   Depositor

   Christopher Potter

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac Dual α1B/β3 Tubulin

   Plasmid

   #170552

   Purpose

   Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

   Depositor

   Tarun Kapoor

   Article

   Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

   Inserts

   TUBA1B (TUBA1B Human)
   TUBB3 (TUBB3 Human)
   

   Use

   Tags

   AP linker, GS linker (GGSGG), His10 tag, L21 enha…

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hy_pUbi-p63e mCherry SV40

   Plasmid

   #176300

   Purpose

   Expresses mCherry mRNA from the Drosophila Ubi-p63e promoter

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ubi-p63e

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx-NTOM20-mVenus-Zdk2

   Plasmid

   #81011

   Purpose

   Expression of Zdk2 anchored to mitochondria

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk2
   

   Use

   Tags

   6x His tag, FLAG, NTOM20, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCFD3-ovoD1

   Plasmid

   #111142

   Purpose

   Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

   Insert

   pCFD3-ovoD1 (ovo Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6:3

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. ovoD1 D1B2NR (18 kb)

   Plasmid

   #37280

   Depositor

   Norbert Perrimon

   Article

   Chou et al Development. 1993 Dec;119(4):1359-69.

   Insert

   ovoD1 genomic DNA fragment
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pFastBac-Dual-M2

   Plasmid

   #135584

   Purpose

   High eukaryotic dual protein expression shuttle vector

   Depositor

   Xiao-Wen Cheng

   Article

   Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

   Type

   Empty backbone

   Use

   Transfer vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin of AcMNPV

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT-ADARcd-E488Q-V5

   Plasmid

   #139686

   Purpose

   Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Adenosine deaminase acting on RNA (Adar Fly)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBPGUw

    Plasmid

    #17575

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Tools for neuroanatomy and neurogenetics

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5XUAS IVS CsChrimson tdtomato_tr

    Plasmid

    #111545

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CsChrimson_tdtomato_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTF023_His_hsDDA1

    Plasmid

    #174480

    Purpose

    Expresses DDA1 in insect cells

    Depositor

    Eric Fischer

    Article

    Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

    Insert

    DDA1 (DDA1 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-NTOM20-mVenus-Zdk1

    Plasmid

    #81010

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIDS-LSD1fl

    Plasmid

    #109157

    Purpose

    Encodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK33/Cas9

    Plasmid

    #133781

    Purpose

    Expresses drosophila-optimized Cas9

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Insert

    Drosophila-optimized Cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB1.HMBP.PrS.MTF2

    Plasmid

    #125169

    Purpose

    Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    MTF2 (MTF2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLIB

    Plasmid

    #80610

    Purpose

    pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLIB-FLAG(N)-RPB1

    Plasmid

    #231722

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    POLR2A (POLR2A Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcGHLT-B-DDB1

    Plasmid

    #48638

    Purpose

    Expresses Full Lenght DDB1 in Insect Cells (ampR)

    Depositor

    Ning Zheng

    Article

    Li et al Cell. 2006 Jan 13;124(1):105-17.

    Insert

    DDB1 (DDB1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pBPHLWL-LexA

    Plasmid

    #26258

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    LexA
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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