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Hy_pMT Flag-URH49 WT

Plasmid

#110127

Purpose

Expresses Flag-tagged human URH49 (DDX39A)

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

URH49 (DDX39A Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

April 6, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-syb:spGFP1–10

Plasmid

#73698

Purpose

Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spGFP1–10 chimera

Use

Drosophila

Tags

Expression

Insect

Mutation

Promoter

Available since

March 22, 2016

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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Copia Renilla Control

Plasmid

#38093

Depositor

Philip Beachy

Article

Lum et al Science. 2003 Mar 28;299(5615):2039-45.

Insert

renilla luciferase

Use

Luciferase

Tags

Expression

Insect

Mutation

Promoter

copia

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pAC-GAL4

Plasmid

#24344

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

GAL4

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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Activin A (S2-2)

Plasmid

#184621

Purpose

for transfection into S2 cells and expression of Activin A protein

Depositor

Timothy Springer

Article

Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

Insert

INHBA (INHBA Human)

Use

Tags

8x His - streptavidin binding peptide

Expression

Insect

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLIB

Plasmid

#80610

Purpose

pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His10TEV

Plasmid

#159427

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Use

Tags

TEV-protease-cleavable His10-tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTP2620

Plasmid

#113311

Purpose

human Rad50 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Rad50 (RAD50 Human)

Use

Tags

HA, 6xhis

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTP813

Plasmid

#113308

Purpose

human Mre11 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

Insert

Mre11 (MRE11 Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFBOT563

Plasmid

#40864

Depositor

Harold Gainer

Article

Fields et al PLoS One. 2012;7(2):e32085. Epub 2012 Fe

Insert

Oxytocin promoter

Use

AAV

Tags

Expression

Bacterial and Insect

Mutation

Promoter

mouse oxytocin promoter

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBPnlsLexA::GADflUw

Plasmid

#26232

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

nlsLexA::GADfl

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pHY002_Strep_Avi_hsDCAF15

Plasmid

#174479

Purpose

Expresses DCAF15 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DCAF 15 (DCAF15 Human)

Use

Tags

StrepII-Avi-TEV

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor WT

Plasmid

#12150

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Use

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Use

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBP-split-intein-Gal4[21-881]-C-int

Plasmid

#199202

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSR657

Plasmid

#65214

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-2 cap

Use

Tags

Expression

Insect

Mutation

Promoter

p10 and polyhedrin

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac_His-ZZ-TEV-Lis1

Plasmid

#132539

Purpose

Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

Depositor

Andrew Carter

Article

Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

Insert

Lis1 (PAFAH1B1 Human)

Use

Tags

8x His, Linker-TEV-linker_TEV, and ZZ

Expression

Insect

Mutation

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

Plasmid

#162388

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Use

Tags

6xHis

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBPp65ADZpUw

Plasmid

#26234

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

p65AD-Zip (RELA Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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