4,958 results

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pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAcGHLT-B-DDB1

Plasmid

#48638

Purpose

Expresses Full Lenght DDB1 in Insect Cells (ampR)

Depositor

Ning Zheng

Article

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

DDB1 (DDB1 Human)

Expression

Insect

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
pP{ELAV-GeneSwitch}

Plasmid

#83957

Purpose

Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

Depositor

Haig Keshishian

Article

Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

Insert

fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Budding Yeast, Human)

Expression

Insect

Promoter

elav

Available Since

Dec. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINE GFP nanobody:Halo:His6

Plasmid

#111090

Purpose

Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

Depositor

Lennart Wirthmueller

Article

Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

Insert

GFP Nanobody

Tags

Halo and His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7 promoter

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ac-AChBP

Plasmid

#63128

Purpose

Expression of Aplysia californica AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pFBOH-LIC_DDB1:1-1140_delta:396-689

Plasmid

#210897

Purpose

Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)

Expression

Insect

Mutation

loop deletion amino acids I396 to D689

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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p4X-UASTattB

Plasmid

#189858

Purpose

Fly cloning and transformation

Depositor

Jung Hwan Kim

Article

Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

Type

Empty backbone

Expression

Insect

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-GFP

Plasmid

#175363

Purpose

ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Insert

sgGFP

Expression

Insect

Mutation

F64L, S65C, and I167T

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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VT028327-FRT10-STOP-FRT10-GAL4

Plasmid

#232829

Purpose

Enhancer VT028327 driven GAL4, gated by Sparse.FRT10

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAST-pHuji-GINKO2

Plasmid

#177117

Purpose

Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

pHuji-GINKO2

Expression

Insect

Promoter

hsp70 promoter

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLIB-GST-3C-CDK1

Plasmid

#177011

Purpose

Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CDK1 (CDK1 Human)

Tags

GST-3C

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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IMPT-10314:GABBR2

Plasmid

#157851

Purpose

HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Article

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABBR2 (GABBR2 Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

Plasmid

#178074

Purpose

Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

MZT2A (MZT2A Human)

Tags

PreScission-mEGFP and ZZ-TEV

Expression

Insect

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBIG1b

Plasmid

#80612

Purpose

pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

Plasmid

#73164

Purpose

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

Depositor

Bruce Edgar

Article

Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

Insert

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)

Tags

mRFP1, GFP

Expression

Insect

Mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAc-C Med12His

Plasmid

#49240

Purpose

expresses human Med12 with His tag in insect cells

Depositor

Dylan Taatjes

Article

Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

Insert

Med12 (MED12 Human)

Tags

His

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pWALIUM10-GW-TurboID-V5

Plasmid

#196283

Purpose

Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies

Depositor

Norbert Perrimon

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pACU2

Plasmid

#31223

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Type

Empty backbone

Expression

Insect

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pAcBac1-BacMam-4x-PyOtR

Plasmid

#217364

Purpose

Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

4 copies of pyrrolysyl-tRNA mutant PyOtR

Use

Baculoviral

Expression

Insect and Mammalian

Promoter

U6

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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ANO10 (TMEM16KA-c001)

Plasmid

#110267

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

TMEM16K (ANO10 Human)

Tags

TEV-His10-Flag

Expression

Insect

Promoter

polyhedrin promoter

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-mMD2-proA

Plasmid

#187878

Purpose

baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

MD2 (Ly96 Mouse)

Expression

Insect

Mutation

includes residues 19–160 of mouse MD-2

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP

Plasmid

#165830

Purpose

EGFP green fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EGFP

Expression

Insect

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP, Asn10

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGBdest-GST-PI3Kc1

Plasmid

#187992

Purpose

Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326

Depositor

Sascha Martens

Inserts

ATG14 (ATG14 Human)
VPS34 (PIK3C3 Human)
VPS15 (PIK3R4 Human)
BECN1 (BECN1 Human)

Tags

GST

Expression

Bacterial and Insect

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pWALIUM10-V5-TurboID-GW

Plasmid

#196285

Purpose

Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies

Depositor

Norbert Perrimon

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAceBAC2-MLG-HMF-LST2

Plasmid

#220787

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in insect cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Tags

10xHis-Myc-FLAG

Expression

Insect

Promoter

p10

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pFL-EGFP-3xFlag-HLTF

Plasmid

#193055

Purpose

Baculovirus expression of human HLTF for recombinant protein production

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

HLTF (HLTF Human)

Tags

3xFlag

Expression

Insect

Promoter

polyhedron

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pVL1392 TMEM97

Plasmid

#157748

Purpose

Baculovirus transfer vectors to create a virus expressing human TMEM97

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Tags

flag

Expression

Insect

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFBOH-MHL_DCAF11:1-546

Plasmid

#210907

Purpose

Insect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

R207H natural variant (VAR_020121)

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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