5,046 results
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Plasmid#68368PurposeExpresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.DepositorInsertDHX36 (DHX36 Human)
TagsHistidineExpressionBacterial, Insect, and Mamm…PromoterCMV ie enhancer/promoterAvailable SinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2
Plasmid#80275PurposeGAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.DepositorInsertQF2
ExpressionInsectAvailable SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 hBRM
Plasmid#1956DepositorAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUAST-pHuji-GINKO2
Plasmid#177117PurposeGenetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusionDepositorInsertpHuji-GINKO2
ExpressionInsectPromoterhsp70 promoterAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWalium20-10XUAS-3XFLAG-dCas9-VPR
Plasmid#78897PurposeExpresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRaDepositorInsertdCas9-VPR
Tags3xFLAGExpressionInsectPromoter10XUASAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427T
Plasmid#81035PurposeMammalian expression of LOV I427T, a mutant with super short relaxation timeDepositorInsertLOV I427T
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427TAvailable SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRB17
Plasmid#52527Purposeserves as template for PCR-mediated fusion of U6-promoter with sgRNADepositorInsertDrosophila U6C promoter
UseCRISPR; Blunt cloning kitTagsT7-promoterExpressionInsectAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pUASTB
Plasmid#18944DepositorInsertphiC31 attB
ExpressionInsectAvailable SinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pBIG2ab
Plasmid#80616PurposepBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-mtdT-3XHA
Plasmid#24354DepositorInsertmtDT-3XHA
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-C450A
Plasmid#22025PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains light-insensitive mutation C4…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFBDM
Plasmid#110738PurposePlasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transpositionDepositorTypeEmpty backboneExpressionInsectPromoterPolyhedrin and p10 promotersAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACU2_CD4-mIFP T2A HO1
Plasmid#72441PurposeExpressing UAS-CD4-mIFP T2A HO1 in drosophilaDepositorInsertCD4-mIFP T2A HO1
ExpressionInsectAvailable SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHsp83-NLS-HA-2xPCP-2xGFP
Plasmid#71243PurposeUsed to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.DepositorInsert2xPCP-2xGFP
UseP element-mediated germline transformationTagsEGFPExpressionInsectPromoterHsp83Available SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pActP-dtrpA1
Plasmid#64713Purposeinducing calcium influx in cultured Drosophila S2 cellsDepositorInsertdtrpA1 (TrpA1 )
ExpressionInsectAvailable SinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbECFP
Plasmid#47917PurposeDonor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.DepositorInsertECFP
TagsNLSExpressionInsectPromoterAedes aegypti polyubiquitin (PUb)Available SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDR121
Plasmid#37148DepositorAvailable SinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10
Plasmid#162386PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
Tags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-MBP-SCov2-nsp12
Plasmid#154759PurposeExpression of SARS-CoV-2 nsp12DepositorInsertSARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionInsectPromoterPolyhedronAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRExpressionInsectPromoterdU6:3Available SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTP813
Plasmid#113308Purposehuman Mre11 in baculovirus transfer vectorDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR BirA
Plasmid#113640PurposeInsect cell expression vector for BirA Biotin LigaseDepositorAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NLyn-Venus-Zdk1
Plasmid#81042Purposemammalian expression of plasma-membrane anchored Zdk1DepositorInsertZdk1
Tags6x His tag, FLAG, NLyn, and mVenusExpressionBacterial, Insect, and Mamm…Available SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo
Plasmid#73164Purposemulti-cistronic Vector for expression for Fly_FUCCI probes in insect cellsDepositorTagsmRFP1, GFPExpressionInsectMutationCyclin B Fragment aa 1-266 & E2F1 Fragment aa…Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHsp83-NLS-HA-2xMCP-2xTagRFP-T
Plasmid#71242PurposeUsed to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.DepositorInsertNLS-2xMCP-2xTagRFP-T
UseP element-mediated germline transformationTagsTagRFP-TExpressionInsectPromoterHsp83Available SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR651
Plasmid#65213PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-1 cap
UseAAVExpressionInsectPromoterp10 and polyhedrinAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only