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Plasmid#110740PurposePlasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transpositionDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only
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LAMP1-GFP
Plasmid#16290DepositorInsertLAMP1/RE72002
UseTagsEGFPExpressionInsectMutationPromoterAc5Available sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBPnlsLexA::p65Uw
Plasmid#26230DepositorInsertnlsLexA::p65 (RELA Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[21-881]-C-int
Plasmid#199202PurposeGateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expressionDepositorInsertsplit-intein-Gal4[21-881]-C-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 V416L
Plasmid#81036Purposemammalian expression of LOV V416L, a mutant with super long relaxation timeDepositorInsertLOV V416L
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416LPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
6X-STAT-synthetic luciferase
Plasmid#37392DepositorInsertsix STAT consensus sites
UseLuciferaseTagsExpressionInsectMutationPromoterHsp70Available sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUAST-pHuji-GINKO2
Plasmid#177117PurposeGenetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusionDepositorInsertpHuji-GINKO2
UseTagsExpressionInsectMutationPromoterhsp70 promoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS ChrimsonR mCherry_tr
Plasmid#111546PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM3
Plasmid#154168PurposeEmpty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk1
Plasmid#81010PurposeExpression of Zdk1 anchored to mitochondriaDepositorInsertZdk1
UseTags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
PUC19_C_tag
Plasmid#186659PurposeEmpty donor plasmid for C-terminal tag of 3xFlag-3xHA.DepositorInsertC-terminal 3xFlag-3xHA Tag
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-TfR
Plasmid#12130DepositorInserttransferrin receptor (TFRC Human)
UseTags6X-His tag, Factor Xa cleavage site, and leader p…ExpressionInsectMutationThe portion of the human TfR gene encoding residu…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10TEV
Plasmid#159427Purposeempty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsTEV-protease-cleavable His10-tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pOmnibac_ZZ_linker_K963_GFP_SNAP
Plasmid#159727PurposeExpresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationPromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual wt p110α/p85α
Plasmid#199302Purposeco-expression of His-tagged p110α wt and Avi-tagged p85α wt proteinsDepositorUseTagsAvi and HisExpressionInsectMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…PromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-Sumo-TEV human OAS1
Plasmid#130895PurposeHuman oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobosDepositorInsertoligoadenylate synthase 1 (OAS1 Human)
UseTagsStrep_SumoExpressionInsectMutationPromoterphAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBPLexA::p65Uw
Plasmid#26231DepositorInsertLexA::p65 (RELA Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
p130Cas Y15F
Plasmid#186154PurposeFor expressing recombinant p130Cas in insect cells.DepositorInsertBCAR1 (Bcar1 Rat)
UseTagsHis6 (Tev)ExpressionInsectMutation15Y residues mutated to AlaninePromoterpolyhedrinAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACgp67B-Her2
Plasmid#10794DepositorInsertErbB2 ScFv (ERBB2 Human)
UseTags6HisExpressionInsectMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-2xHA-MOF
Plasmid#29763DepositorInsertMYST1 (KAT8 Human)
UseTags2xHAExpressionInsectMutationPromoterAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
LRP1A-c004
Plasmid#205112PurposeLRP1 protein expressionDepositorInsertLRP1 (LRP1 Human)
UseTagsAviTag and GP64sp-6His-TEVExpressionInsectMutationWTPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFBOT563
Plasmid#40864DepositorInsertOxytocin promoter
UseAAVTagsExpressionBacterial and InsectMutationPromotermouse oxytocin promoterAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-pGC-A
Plasmid#186626PurposepFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)DepositorInsertNPR1 (NPR1 Human)
UseTagshemagglutinin (HA) signal peptide + His10-tag + T…ExpressionInsectMutationdeleted amino acids 1-32PromoterpolyhedringAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP N10 TEV LIC cloning vector (4C)
Plasmid#30116DepositorTypeEmpty backboneUseTagsHis6-MBP-N10-TEVExpressionInsectMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBAC-ECFP-15xQUAS_TATA-SV40
Plasmid#104875PurposepiggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformationDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHY002_Strep_Avi_hsDCAF15
Plasmid#174479PurposeExpresses DCAF15 in insect cellsDepositorInsertDCAF 15 (DCAF15 Human)
UseTagsStrepII-Avi-TEVExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only