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pEF5B-FRT-GFP-αTAT1[D157N]

Plasmid

#27100

Depositor

Maxence Nachury

Article

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

αTubulin K40 acetyltransferase (ATAT1 Human)

Tags

GFP and S-tag

Expression

Mammalian

Mutation

D157N

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-GFP

Plasmid

#85556

Purpose

2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-GFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag3HuR

Plasmid

#135950

Purpose

Expresses HuR in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

Flag3

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-NBR1

Plasmid

#38283

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Next to BRCA1 gene 1 protein (NBR1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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p2,0_3M5M_eGFP

Plasmid

#135474

Purpose

Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

Marburg virus minigenome, eGFP reporter

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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NES-Grx1-roGFP2

Plasmid

#236551

Purpose

Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

Depositor

Pablo Rivera-Fuentes

Insert

NES-Grx1-roGFP2

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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p23-puro-EGFP-RPA194

Plasmid

#179237

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

RPA194 (POLR1A Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_HA

Plasmid

#178534

Purpose

Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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hCas9

Plasmid

#41815

Purpose

Expresses human codon optimized Cas9 nuclease for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1

Plasmid

#52326

Purpose

expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R117Q mutation (R95Q according to numbering in Fi…

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV6-AC EJ7-GFP

Plasmid

#113617

Purpose

Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

EJ7-GFP variant of EGFP

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab11a-7

Plasmid

#55124

Purpose

Localization: Ras GTPase, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Rab11a (RAB11A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pInducer10-mir-RUP-PheS

Plasmid

#44011

Purpose

Lentiviral shRNA vector (pGIPz based) for inducible knockdown

Depositor

Stephen Elledge

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Promoter

TRE2

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3 N-MYC TIP60

Plasmid

#213003

Purpose

expresses human TIP60

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

KAT5 (KAT5 Human)

Tags

MYC

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS2-Spike

Plasmid

#145032

Purpose

Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV-2 Spike (S Synthetic)

Tags

C9

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGL4-ERSE2-luc2P-Hygro

Plasmid

#101790

Purpose

Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)

Depositor

Seiichi Oyadomari

Article

Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

Insert

3xERSE-II

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA

Plasmid

#105005

Purpose

Mammalian Expression of PDZD8

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX-MCS UBC promoter

Plasmid

#128045

Purpose

Lentiviral vector with UBC promoter

Depositor

Oskar Laur

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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NanoLuc-EP300 Fusion Vector

Plasmid

#238648

Purpose

Express NanoLuc-EP300 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

EP300 (EP300 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO GFP HSPA1A

Plasmid

#19483

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1A (HSPA1A Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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