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3xHA-TurboID-NLS_pCDNA3

Plasmid

#107171

Purpose

expresses 3xHA-tagged TurboID in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-osTIR1-9xMyc-Zeo

Plasmid

#225696

Purpose

Lentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance. For use with AID degron.

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

osTIR1

Use

Lentiviral and Synthetic Biology

Tags

9X Myc

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FDIO-ArchT-GFP

Plasmid

#124640

Purpose

AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

ArchT

Use

AAV; Flp/frt

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-shp38

Plasmid

#52920

Purpose

expresses a hairpin specific for human p38MAPK

Depositor

Sheila Stewart

Article

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting mitogen-activated protein kinase p38 (MAPK14 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-puro Vps34-GFP

Plasmid

#38268

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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PRS_8-eGFP

Plasmid

#192589

Purpose

AAV-vector for fluorescent labeling of noradrenergic neurons

Depositor

Simon Wiegert

Article

Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

PRSX8

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-ER-PM-P2A

Plasmid

#214893

Purpose

Expresses equimolar Plasma Membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PM Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-ER-PM-P2A

Tags

none

Expression

Bacterial and Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Hygro_RGECO1.2

Plasmid

#164592

Purpose

Stable integration and expression of RGECO1.2 in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

R-GECO1.2

Use

Lentiviral

Expression

Mammalian

Mutation

R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-MCL1

Plasmid

#202663

Purpose

Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Neurofascin (extracellular) [A12/18R]

Plasmid

#177438

Purpose

Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLV puro Tet VSVG

Plasmid

#82724

Purpose

Lentiviral plasmid that expresses VSVG protein under a doxycycline-inducible promoter

Depositor

Warren Zipfel

Insert

VSVG

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pIVT-PuroR-P2A-iCre-polyA_pKG03717

Plasmid

#246369

Purpose

IVT template for making modRNA encoding PuroR-P2A-iCre

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

PuroR-P2A-iCre

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCMVNeoDEST(705-1)-hAXL

Plasmid

#124796

Purpose

Expresses AXL in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKH3-TRIM28-C65A/C68A

Plasmid

#92199

Purpose

Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

Tif1b (TRIM28 Synthetic, Human)

Tags

HA

Expression

Mammalian

Mutation

Changed Cysteines 65 and 68 to Alanines.

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX LYCHOS-FLAG-TiD

Plasmid

#199747

Purpose

Lentiviral construct to stably express LYCHOS (GPR155) TurboID

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Tags

Flag and TurboID

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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Perturb-FISH

Plasmid

#220626

Purpose

expression of gRNAs under U6T7 promoter

Depositor

Samouil Farhi

Article

Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

BsmBI

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ER-EBFP2

Plasmid

#231183

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP8

Plasmid

#18066

Depositor

Edward Yeh

Article

Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

Insert

SENP8 (SENP8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only