82,527 results
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Plasmid#85447PurposeExpresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cellsDepositorInsertDHFR-SpCas9-DHFR
UseAAVTagsDestabilized domain (C-terminus version) of E.col…ExpressionMammalianPromoterCbhAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB53x EF1-Dendra2-RPB1Amr
Plasmid#81228Purposeexpresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac systemDepositorInsertDendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
TagsDendra2ExpressionMammalianMutationN792D alpha amanitin resistance mutation (Bartolo…PromoterEF1aAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TDsmURFP-IRES-eGFP
Plasmid#80344PurposeExpresses Tandem Dimer smURFP and eGFP in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTandem Dimer smURFP
eGFP
ExpressionMammalianPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIκBα-miRFP703
Plasmid#80005PurposeIκBα fused with near-infrared fluorescent protein miRFP703 (IκBα reporter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIκBα (NFKBIA Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3_erGAP3
Plasmid#78118PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserterGAP3
TagsKDEL and calreticulin signal peptideExpressionMammalianMutationS175G, D180Y, I167V and L15Q in GFP moiety and D…PromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NLS-mCherry-NES reporter (pDN160)
Plasmid#72660PurposeNLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-mCherry-NES
ExpressionMammalianPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5FRT-EF-Pdgfrbeta-EGFPN
Plasmid#66790PurposeExpression of murine PDGFRbeta tagged with GFP under the control of EF1a promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPDGFRbeta (Pdgfrb Mouse)
TagsC-term EGFPExpressionMammalianPromoterEF1aAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP_CD47_SU
Plasmid#65473PurposeGFP tagged CD47 with its own short 3'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSignal peptide of CD47
GFP
CD47 coding region continued and short 3’UTR.
ExpressionMammalianAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_neomycin
Plasmid#65306PurposeLuminal ER hook only (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
TagsKDEL motif and Signal SequenceExpressionMammalianPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRISPR-SP-Cas9 reporter
Plasmid#62733PurposeMammalian CRISPR-SP-Cas9 reporterDepositorInsertCRISPR-SP-Cas9 target seq + tdTomato (out of frame)
UseCRISPR and LentiviralTagsCRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…ExpressionMammalianPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-eGFP-Reporter
Plasmid#60719PurposeExpresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
UseCRISPRExpressionMammalianPromoterU6 and mouse U6Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUltra-puro-RTTA3
Plasmid#58750Purposesilence doxycyclin inducible pUltra-doxDepositorInsertReverse TET-Transactivator 3
UseLentiviralExpressionMammalianMutationloxP5171PromoterUbiquitin C promoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRExpressionMammalianAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Sequestosome1-N-18
Plasmid#55132PurposeLocalization: Membrane Scaffold, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSequestosome1 (SQSTM1 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C3-Sec5
Plasmid#53756PurposepEGFP-C3 plasmid that expresses Sec5DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec5 (EXOC2 Human)
TagsGFPExpressionMammalianAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEos2-C1
Plasmid#54510PurposeLocalization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneTagsmEos2ExpressionMammalianAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA3.1 3xFlag IFIT3
Plasmid#53553Purposemammalian expression of IFIT3DepositorAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBIR-GFP
Plasmid#49807PurposeIn vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
ExpressionMammalianPromoterbeta-actinAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA
Plasmid#47457PurposeLITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS(alpha-imp)-CRY2PHR-NLS-VP64
Tags2A_GFPExpressionMammalianPromoterEF1-alphaAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSimpleII-NLS-NmCas9-HA-NLS(s)
Plasmid#47867PurposeThis plasmid encode NmCas9 with two NLS and a HA tagDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNmCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterEF1aAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only