81,065 results

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pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

Plasmid

#45574

Purpose

Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

co I-Sce I

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-VSFP Butterfly 1.2

Plasmid

#47978

Purpose

Genetically encoded voltage indicator

Depositor

Thomas Knopfel

Article

Akemann et al J Neurophysiol. 2012 Oct;108(8):2323-37.

Insert

VSFP Butterfly 1.2

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pU6-sgGAL4-1

Plasmid

#46915

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-1
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pAC153-dual-dCas9VP96-sgExpression

Plasmid

#48239

Purpose

Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP96

Expression

Mammalian

Mutation

D10A H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pOTTC374 - pAAV EF1a DIO iRFP

Plasmid

#47626

Purpose

An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pMIGR AMPK KD

Plasmid

#27296

Depositor

Morris Birnbaum

Insert

AMP Acticated Protein Kinase (Prkaa2 Rat)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Kinase Dead = Lys 45 to Arg

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-3myc-6His-EZH2 21D

Plasmid

#42664

Depositor

Mien-Chie Hung

Article

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Insert

EZH2 (EZH2 Human)

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21D

Available Since

July 17, 2013

Availability

Academic Institutions and Nonprofits only
MAP2-GCaMP3

Plasmid

#43917

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

GCaMP3

Use

Lentiviral

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

E247V in GCaMP3

Promoter

Human MAP2 promoter

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd5-1D4

Plasmid

#42267

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 5 (Fzd5 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pAquaN1

Plasmid

#42888

Depositor

Fabienne Merola

Article

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Expression

Mammalian

Mutation

T66S and H149G

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pPTP1BD181A-mCherry

Plasmid

#40270

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

PTPN1 (PTPN1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D181A

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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perbB1-Citrine

Plasmid

#40266

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB1 (EGFR Human)

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-KLF5-his

Plasmid

#40904

Depositor

Jin-Tang Dong

Article

Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Insert

Kruppel-like factor 5 (KLF5 Human)

Tags

HA and His

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-P85

Plasmid

#40219

Depositor

Lynda Chin

Insert

P85 alpha (PIK3R1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-N GFP

Plasmid

#37855

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

GFP

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
Arf5-GFP

Plasmid

#39557

Depositor

Paul Melancon

Article

Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

Insert

ADP ribosylaion factor 5 (ARF5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pWPXL-c-Myc

Plasmid

#36980

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

c-Myc (Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-N 16E5

Plasmid

#37874

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16 E5 (E5 )

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 25, 2012

Availability

Academic Institutions and Nonprofits only
pWPI/hPLK2WT/Neo

Plasmid

#35385

Depositor

Anurag Tandon

Article

Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

Insert

hPLK2 (PLK2 Human)

Use

Lentiviral

Tags

EMCV IRES-Neomycin cassette and V5 epitope and Po…

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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Pax2

Plasmid

#35002

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Paired box protein Pax-2 (Pax2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG:myr-Venus

Plasmid

#32602

Depositor

Anna-Katerina Hadjantonakis

Article

Rhee et al Genesis. 2006 Apr;44(4):202-18.

Insert

myr-Venus

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pCGN-MEF2D

Plasmid

#32963

Purpose

For mammalian expression of mouse MEF2D with an HA tag

Depositor

Ron Prywes

Insert

MEF2D (Mef2d Mouse)

Tags

HA

Expression

Mammalian

Mutation

E287G

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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CMV500 A-C/EBPβ

Plasmid

#33363

Depositor

Charles Vinson

Insert

Cebpb (Cebpb Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see comments)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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ptyrEGFP-TYR

Plasmid

#32782

Depositor

Ruth Halaban

Article

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Insert

Tyrosinase (TYR Human)

Tags

EGFP

Expression

Mammalian

Promoter

Tyrosinase

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
p6345 MSCV-CMV-Flag-HA-Brd4 1-722

Plasmid

#31352

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

*see comments

Available Since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 S259A/S498A

Plasmid

#32216

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser259Ala, Ser498Ala

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG 17xS/T->A, catalytic active lipin 1

Plasmid

#32007

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

Promoter

CMV, SP6

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
FLX1.8MitoKaede

Plasmid

#28133

Depositor

Geoffrey Owens

Insert

MitoKaede

Use

Lentiviral

Tags

COX VIII signal sequence

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1his p66shc S36A (corrected)

Plasmid

#32575

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Tags

His

Expression

Mammalian

Mutation

S36A

Promoter

CMV

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF1-GRM3 WT

Plasmid

#31798

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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81,065 results