79,341 results

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TFORF2198

Plasmid

#142924

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MLXIPL (MLXIPL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR77:1-342

Plasmid

#211169

Purpose

Mammalian expression of full-length human WDR77. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR77:M1-E342 (WDR77 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVUTHshGATA1-tTR-KRAB

Plasmid

#11650

Purpose

Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin promoter) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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MAC_N_ARPC1B

Plasmid

#111681

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

ARPC1B (ARPC1B Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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evoBE4-NG

Plasmid

#226852

Purpose

Expresses evoBE4max-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

evoBE4max-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pUltraHot-mCherry-DHX36-iso1-WT

Plasmid

#206964

Purpose

expressing the protein DHX36 fused to mCherry in human cells

Depositor

Eran Hornstein

Article

Danino et al Nucleic Acids Res. 2023 Jul 28:gkad613.

Insert

DEAH-Box Helicase 36 (DHX36 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IGSF8-Fc(DAPA)-AviTag-6xHis

Plasmid

#156912

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IGSF8 (IGSF8 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N

Plasmid

#80930

Purpose

Expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

Plasmid

#210709

Purpose

This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN

Use

AAV and CRISPR

Expression

Mammalian

Mutation

N55K in MCP

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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CDK1as_T2A_Zeo

Plasmid

#118596

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-TPC2

Plasmid

#80153

Purpose

Intracellular Ca2+ store marker

Depositor

Santiago Di Pietro

Article

Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

Insert

TPCN2 (TPCN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Myo10

Plasmid

#135403

Purpose

Expresses GFP-tagged bovine Myo10 in mammalian cells.

Depositor

Richard Cheney

Article

Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

Insert

Myo10 (MYO10 Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

"Relative to the GenBank Myo10 cDNA sequence…

Promoter

CMV

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSicoR-mCh-empty

Plasmid

#21907

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-ExRai-AKAR2

Plasmid

#171846

Purpose

Cre-dependent, synapsin promoter-driven expression of the green fluorescent ExRai-AKAR2 PKA biosensor in neurons.

Depositor

Richard Huganir

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-3xT7

Plasmid

#171028

Purpose

Expresses 3xT7-tagged target in mammalian cells

Depositor

Kai Ge

Article

Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

Type

Empty backbone

Use

Retroviral

Tags

3x-T7

Expression

Mammalian

Promoter

5' LTR

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pWPXL-Sox9

Plasmid

#36979

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2012

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG[EF1α-CD19 4-1BB-eGFP]

Plasmid

#218344

Purpose

Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_UCN1.0

Plasmid

#208667

Purpose

Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCG-TPMV Fd32

Plasmid

#163964

Purpose

Expression of TPMV F glycoprotein in mammalian cells

Depositor

Jakob Reiser

Article

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

TPMV F glycoprotein

Expression

Mammalian

Mutation

Lacking 32 amino acids at C-terminus

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP30_EBOV

Plasmid

#103051

Purpose

Encodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

VP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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