78,133 results

1. pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

   Plasmid

   #196971

   Purpose

   Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

   Depositor

   Leopold Parts

   Inserts

   Cas9-RT
   rtTA3G-P2A-eGFP
   

   Expression

   Mammalian

   Promoter

   PGK and TREG3G

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0169

   Plasmid

   #141540

   Purpose

   Lentiviral vector for overexpressing the SIX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SIX3 (SIX3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-hAsCpf1(TATV) (pY220)

   Plasmid

   #89353

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TATV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RVR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K548V/N552R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AcGFP1-N1

   Plasmid

   #54705

   Purpose

   Localization: N1 Cloning Vector, Excitation: 475, Emission: 505

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   AcGFP1

   Expression

   Mammalian

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. FASTHDR-Cterm-mClover3-Puromycin

   Plasmid

   #167205

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mClover3

   Expression

   Mammalian

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCer-Mcm5

   Plasmid

   #211391

   Purpose

   mammalian expression of human Mcm5 N-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   Mcm5 (MCM5 Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-WT-VWF

   Plasmid

   #124794

   Purpose

   Expresses multimeric human VWF with C-terminal c-Myc tag

   Depositor

   Sriram Neelamegham

   Article

   Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

   Insert

   VWF (VWF Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-GPHN-FingR-mGold2s-CCR5TC

   Plasmid

   #231785

   Purpose

   Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   GPHN-FingR-mGold2s-CCR5TC
   

   Expression

   Mammalian

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

   Plasmid

   #194681

   Purpose

   CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01-mEGFP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRB-NLuc

    Plasmid

    #31181

    Depositor

    David Piwnica-Worms

    Article

    Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pClneoMyc rat S-SCAM alpha

    Plasmid

    #40213

    Depositor

    Yutaka Hata, Yoshimi Takai

    Article

    Hirao et al J Biol Chem. 1998 Aug 14;273(33):21105-1

    Insert

    S-SCAM (Magi2 Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pRST7-ER beta (long)

    Plasmid

    #11356

    Depositor

    Donald McDonnell

    Article

    Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

    Insert

    ER beta long (ESR2 Human)
    

    Expression

    Mammalian

    Available Since

    March 10, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pAT-WT-Myc

    Plasmid

    #177717

    Purpose

    ER protein alpha-1-antitrypsin (WT), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS/ES-EnvA-VSVg-WPRE-pA

    Plasmid

    #87221

    Purpose

    to package wild type EnvA psudotyped lentivirus

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    EnvA-VSVg
    

    Use

    Mouse Targeting and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-DDX3X

    Plasmid

    #70648

    Purpose

    Expresses WT DDX3X in mammalian cells under blasticidin selection.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Retroviral

    Tags

    3x FLAG

    Expression

    Mammalian

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCAG_iPE-C

    Plasmid

    #214735

    Purpose

    encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_iPE-C_bGH
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Halo-OPTN

    Plasmid

    #187900

    Purpose

    Mammalian cell expression of Halo-tagged OPTN

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    OPTN (OPTN Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2494

    Plasmid

    #142112

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB18 (ZBTB18 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVAX1-BMP2

    Plasmid

    #137909

    Purpose

    Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

    Depositor

    Georg Feichtinger

    Article

    Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

    Insert

    BMP2 (BMP2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. ancBE4-NG

    Plasmid

    #226853

    Purpose

    Expresses ancBE4max-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ancBE4max-NG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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