74,919 results

1. lenti EF1a-FLAG-dCas9-VPR

   Plasmid

   #114195

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mfn2-Myc

   Plasmid

   #23213

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Mfn2 (Mfn2 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. mRNA-CleanCap-NanoLuc

   Plasmid

   #188247

   Purpose

   Template plasmid to transcribe mRNA encoding NanoLuc

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   NanoLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PPRE X3-TK-luc

   Plasmid

   #1015

   Purpose

   PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

   Depositor

   Bruce Spiegelman

   Article

   Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

   Insert

   PPRE
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCmini-iCAP-AAV1.X1

   Plasmid

   #209780

   Purpose

   non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

   Insert

   Synthetic construct isolate AAV1-X1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

   Plasmid

   #234952

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234953

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. TLCV2-Scal-T2A-EGFP-Puro

   Plasmid

   #234949

   Purpose

   Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. TLCV2-ApaLI-T2A-EGFP-Puro

   Plasmid

   #234948

   Purpose

   Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.3/V5-DEST-GFP

    Plasmid

    #40125

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-HIF1 alpha

    Plasmid

    #21101

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-puro-cOVA-IRES-BFP

    Plasmid

    #135074

    Purpose

    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.

    Depositor

    Maria Castro

    Insert

    cOVA-IRES-BFP (OVAL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. phU6-gRNA

    Plasmid

    #53188

    Purpose

    Expresses the S. pyogenes sgRNA from the human U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid

    #55201

    Purpose

    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. GPR161-Tango

    Plasmid

    #66334

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR161 (GPR161 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. Halo_aTubulin-pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221901

    Purpose

    Expresses Halo7 fused to alpha tubulin

    Depositor

    Janelia Integrative Imaging

    Insert

    Halo7-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-HA-mSTING

    Plasmid

    #214157

    Purpose

    Transient expression of STING

    Depositor

    Nan Yan

    Article

    Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

    Insert

    STING (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge