78,292 results

1. cyto-iATPSnFR1.0

   Plasmid

   #102550

   Purpose

   cytoplasmic ATP sensor for mammalian cells

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Expression

   Mammalian

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV CMV-dSaCas9-KRAB-bGHpA

   Plasmid

   #106219

   Purpose

   AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-3xFLAG-GFP-puroR

   Plasmid

   #167463

   Purpose

   3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

   Depositor

   Steven Artandi

   Article

   Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX_DSB_Spectrum-V1

   Plasmid

   #192474

   Purpose

   Lentiviral DSB Spectrum V1 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. cytoplasmic cpVenus control

   Plasmid

   #186788

   Purpose

   Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   cytoplasmic cpVenus control
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-eGFP

   Plasmid

   #36083

   Purpose

   3rd generation lentiviral transfer plasmid

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-KRAB-MeCP2

   Plasmid

   #110821

   Purpose

   Improved dCas9 repressor-dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-Puro-AAVR-FLAG

   Plasmid

   #166716

   Purpose

   Vector expressing AAVR (KIAA0319L) with a C-terminal Flag tag

   Depositor

   Jan Carette

   Article

   Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

   Insert

   AAVR-FLAG (KIAA0319L Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1 neo

   Plasmid

   #13425

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.

   Depositor

   Sheila Stewart

   Insert

   non-hairpin 18bp
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Dec. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. MaLionG

    Plasmid

    #113906

    Purpose

    Green ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionG
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. gag-COM-pol

    Plasmid

    #211373

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-COM-pol
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330S-2-PITCh

    Plasmid

    #63670

    Purpose

    Expresses Cas9 nuclease and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABE8.20-SpRY

    Plasmid

    #226859

    Purpose

    Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid

    #179105

    Purpose

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-luciferase-P2A-Neo

    Plasmid

    #105621

    Purpose

    3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. attB-puro donor.dna

    Plasmid

    #181923

    Purpose

    attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Expression

    Mammalian

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. gag-P3-pol

    Plasmid

    #211374

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-P3-pol
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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