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Mammalian Expression

76,966 results

Showing: 1501 - 1520 of 76966 results

  1. TFORF0208

    Plasmid
    #142556
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP2 (FOXP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    142556-plasmid-map-sequence-id-427312

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  2. NanoLuc-PM

    Plasmid
    #164784
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
    Use
    Tags
    NanoLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164784-plasmid-map-sequence-id-326673

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  3. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234453
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234453-plasmid-map-sequence-id-465748

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  8. pIRE4-Bpa

    Plasmid
    #155342
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    humanized EBpaRS/BstYam
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK/U6
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155342-plasmid-map-sequence-id-310519

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  10. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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Showing: 1501 - 1520 of 76966 results