76,419 results

1. pCS2-MT GLI2 FL

   Plasmid

   #17648

   Depositor

   Erich Roessler

   Article

   Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

   Insert

   GLI2 (GLI2 Human)
   

   Use

   Tags

   6x Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. PDE9A_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176945

   Purpose

   Mammalian expression vector encoding PDE9A and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   PDE9A (PDE9A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEFIRES-P-EGFP-ADRP

   Plasmid

   #87157

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   mCherry-R TTGGTCACCTTCAGCTTGG

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. P2RY6-Tango

   Plasmid

   #66476

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   P2RY6 (P2RY6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. STARR-seq luciferase validation vector_ORI_empty

   Plasmid

   #99297

   Purpose

   Luciferase validation vector; empty vector without insert to determine baseline

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fuw-dCas9-Tet1CD-P2A-BFP

   Plasmid

   #108245

   Purpose

   Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A

   Promoter

   Ubc

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMVHA EED wt

   Plasmid

   #24231

   Purpose

   Mammalian expression of human EED with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EED (EED Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FKBP-V5-AP-nes_pLX304

   Plasmid

   #120912

   Purpose

   Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   FKBP-V5-AP-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-mCherry-IRES-Dre

   Plasmid

   #55633

   Purpose

   Expresses Dre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Inserts

   mCherry
   Dre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-GRP78/BiP

    Plasmid

    #32701

    Depositor

    Richard C. Austin

    Article

    Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

    Insert

    GRP78/BiP (HSPA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-mCherry-(F30-2xPepper)10

    Plasmid

    #129401

    Purpose

    mCherry mRNA reporter tagged with (F30-2xPepper)10

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    mCherry-(F30-2xPepper)10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-Nucleolin

    Plasmid

    #28176

    Depositor

    Michael Kastan

    Article

    Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-DsRed_IRES_SNCA:EGFP

    Plasmid

    #92195

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-SNCA-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

15. ADRA2B-Tango

    Plasmid

    #66217

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADRA2B (ADRA2B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-Kif2A

    Plasmid

    #52401

    Purpose

    Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

    Depositor

    Gohta Goshima, Ryota Uehara

    Article

    Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

    Insert

    Kif2A (KIF2A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Slug_pGL2

    Plasmid

    #31695

    Depositor

    Paul Wade

    Article

    Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

    Insert

    Slug (SNAI2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV

    Plasmid

    #24828

    Depositor

    Lin He

    Article

    Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-Mff

    Plasmid

    #49153

    Purpose

    mammalian expression of Mff fused to GFP

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    Mff (MFF Human)
    

    Use

    Tags

    acGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2/DEST/hTfR-HA

    Plasmid

    #69610

    Purpose

    human transferrin receptor with an HA tag fused at the C-terminus

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    human transferrin receptor (TFRC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. σ2-mCherry

    Plasmid

    #186579

    Purpose

    Expression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherry

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP2S1 (AP2S1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CALM-pmCherryN1

    Plasmid

    #27691

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    CALM (Picalm Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hSTARR-seq_ORI vector

    Plasmid

    #99296

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LRRK2-WT

    Plasmid

    #17609

    Depositor

    Ted Dawson

    Article

    West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFB2B

    Plasmid

    #195284

    Purpose

    Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-mRuby2

    Plasmid

    #40260

    Purpose

    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    mRuby2
    

    Use

    Tags

    His6 and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85008

    Purpose

    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-α-cateninWT

    Plasmid

    #229709

    Purpose

    Transient expression of GFP-alpha-cateninWT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-3xFLAG-TurboID-MCS

    Plasmid

    #186813

    Purpose

    CAG promoter-driven expression of TurboID-fused protein (N terminus)

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV4-ApoE3

    Plasmid

    #87086

    Purpose

    Expresses ApoE3 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E3 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113610

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    beta 2 adrenergic receptor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PX458-3xHA-FnCas9

    Plasmid

    #130969

    Purpose

    3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    FnCas9
    

    Use

    CRISPR

    Tags

    3xHA-NLS and NLS-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid

    #209079

    Purpose

    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    sfGFP
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR.hU6hH1

    Plasmid

    #32686

    Purpose

    dual shRNA expression cassette entry vector

    Depositor

    Ming-Sound Tsao

    Article

    Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6hH1

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-GST-IRP1

    Plasmid

    #216827

    Purpose

    Expresses human IRP1 with a N-terminal GST-tag in mammalian cells.

    Depositor

    Samie Jaffrey

    Insert

    ACO1 (ACO1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAGGS-PKCdelta-EGFP

    Plasmid

    #178856

    Purpose

    Mammalian expression of PKCδ fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ (PRKCD Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pmax pona 12xTRICK 24xMS2SL

    Plasmid

    #64542

    Purpose

    TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    12x PP7 stem-loops
    

    Use

    Tags

    24xMS2 stem loops, HA, NLS, and dNP799

    Expression

    Mammalian

    Mutation

    Promoter

    ponA

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PCDNA3-GFP1-9

    Plasmid

    #121684

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    GFP1-9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Z-lock αTAT

    Plasmid

    #175290

    Purpose

    light-controlled analog of the alpha-tubulin acetylase αTAT

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Z-lock αTAT
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    αTAT (236 amino acids)

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge