Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Mammalian Expression

76,055 results

Showing: 4251 - 4300 of 76055 results

  8. mNeptune2-Tubulin-6

    Plasmid
    #56149
    Purpose
    Localization: Microtubules, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    mNeptune2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56149-plasmid-map-sequence-id-209084

    Enlarge

  9. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

    Enlarge

  10. SmBiT-b4GALT1 Fusion Vector

    Plasmid
    #237043
    Purpose
    Express SmBiT-b4GALT1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    b4GALT1 (B4GALT1 Human)
    Use
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237043-plasmid-map-sequence-id-471899

    Enlarge

  11. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

    Enlarge

  13. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid
    #228253
    Purpose
    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228253-plasmid-map-sequence-id-451024

    Enlarge

  16. hSyn-eGFP-DDHD2

    Plasmid
    #232463
    Purpose
    Expresses GFP tagged DDHD2 lipase in neurons
    Depositor
    Timothy Ryan
    Insert
    DDHD2 (DDHD2 Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232463-plasmid-map-sequence-id-460234

    Enlarge

  17. mSirt1-GFP

    Plasmid
    #221511
    Purpose
    Fluorescently tagged mouse Sirt1-GFP
    Depositor
    Alessandro Ori
    Insert
    Sirt1 (Sirt1 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221511-plasmid-map-sequence-id-439387

    Enlarge

  18. pLNHA-C1-HseIF4G_O

    Plasmid
    #147087
    Purpose
    Mammalian Expression of HseIF4G
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4G (EIF4G1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation M432V compared to the seu…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147087-plasmid-map-sequence-id-289534

    Enlarge

  20. pLNHA-C1-HseIF4AI_K

    Plasmid
    #146731
    Purpose
    Mammalian Expression of HseIF4AI
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4AI (EIF4A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    a non silent mutation (A200V) and 3 silent mutati…
    Promoter
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    146731-plasmid-map-sequence-id-357210

    Enlarge

  31. LifeAct-mNeonGreen

    Plasmid
    #98877
    Purpose
    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    LifeAct
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98877-plasmid-map-sequence-id-191163

    Enlarge

  35. 6HisVDAC1/pCDNA3

    Plasmid
    #197320
    Purpose
    Plasmid expressing an antigen targeting the VDAC1 transporter
    Depositor
    Dan Minor
    Insert
    VDAC1 (VDAC1 Human)
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    197320-plasmid-map-sequence-id-388161

    Enlarge

  36. pCDH-EF1-IRES eGFP

    Plasmid
    #128059
    Purpose
    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128059-plasmid-map-sequence-id-256675

    Enlarge

  43. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid
    #197502
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197502-plasmid-map-sequence-id-386612

    Enlarge

  46. pBa-MARK2-eGFP

    Plasmid
    #66706
    Purpose
    mammalian expression vector for MARK2 with a C-terminal eGFP
    Depositor
    Marvin Bentley, Gary Banker
    Insert
    MARK2 (MARK2 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    chicken beta actin
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    66706-plasmid-map-sequence-id-114008

    Enlarge

  47. pBABE

    Plasmid
    #21836
    Depositor
    David Ron
    Insert
    EGFP
    Use
    Retroviral
    Tags
    farnysylation signal
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21836-plasmid-map-sequence-id-10029

    Enlarge

Showing: 4251 - 4300 of 76055 results