78,292 results

1. Factor IX donor

   Plasmid

   #182141

   Purpose

   Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
   

   Expression

   Mammalian

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Neo

   Plasmid

   #127762

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPCX mito Grx1-roGFP2

   Plasmid

   #64977

   Purpose

   Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. dead Sa Cas9

   Plasmid

   #138162

   Purpose

   For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   dSaCas9
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D10A + N580A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pink Flamindo

   Plasmid

   #102356

   Purpose

   biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

   Insert

   Pink Flamindo
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hRXRα

   Plasmid

   #135910

   Purpose

   Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Human retinoid X receptor- alpha (RXRA Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTagRFP657-N1

   Plasmid

   #31959

   Depositor

   Vladislav Verkhusha

   Article

   Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

   Insert

   TagRFP657
   

   Expression

   Mammalian

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190034

   Purpose

   vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13d
   

   Expression

   Mammalian

   Mutation

   A134V,A140V,A141V,A143V

   Promoter

   CBh, SV40, hU6

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCP-APEX2

   Plasmid

   #154936

   Purpose

   NLS-V5-MCP-APEX2

   Depositor

   Alice Ting

   Article

   Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

   Insert

   NLS-V5-MCP-APEX2
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-RLuc8

    Plasmid

    #87121

    Purpose

    Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.

    Depositor

    Sanjiv Sam Gambhir

    Article

    Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-Talin head-SpyCatcher003

    Plasmid

    #133566

    Purpose

    Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    EGFP-Talin head-SpyCatcher003
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV500 empty vector

    Plasmid

    #33348

    Depositor

    Charles Vinson

    Article

    Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-miRFP670nano3

    Plasmid

    #184664

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-tdTomato

    Plasmid

    #28306

    Purpose

    Cre-dependent AAV-mediated expression of tdTomato under the CAG promoter

    Depositor

    Edward Boyden

    Has Service

    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    tdTomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV6-mPER2-luc

    Plasmid

    #212035

    Purpose

    Expression of murine Per2-luciferase in mammalian cells

    Depositor

    David Hazlerigg

    Article

    Appenroth et al J Biol Rhythms. 2024 Dec;39(6):554-567.

    Insert

    mPer2-luciferase (Per2 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDL025_HA-rb_IgG_HC_15F11

    Plasmid

    #193622

    Purpose

    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti_HA_15F11 rabbit heavy chain
    

    Tags

    signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDL026_HA-rb_IgG_LC_15F11

    Plasmid

    #193623

    Purpose

    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-HA_15F11 rabbit light chain
    

    Tags

    signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLPCX cyto Grx1-roGFP2

    Plasmid

    #64975

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5B-Flag-Smad3

    Plasmid

    #11742

    Depositor

    Jeff Wrana

    Article

    Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

    Insert

    SMAD3 (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 7, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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