Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Mammalian Expression Clear filter

Applied Filters

Mammalian Expression Clear filter

80,559 results

Showing: 3921 - 3960 of 80559 results

  1. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid
    #218629
    Purpose
    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    mitoEcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and MTS
    Expression
    Mammalian
    Available Since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218629-plasmid-map-sequence-id-432199

    Enlarge

  2. pLVX-TetOne-bsd-atpA

    Plasmid
    #248065
    Purpose
    Lentiviral expression of alpha subunit of ATPGobble. Includes ampR and bsdR (blasticidin). Use together with pLVX-TetOne-bsd-atpD (#248066) and pLVX-TetOne-atpG (#248067) for active ATPGobble.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpA
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in human cells
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248065-plasmid-map-sequence-id-493789

    Enlarge

  3. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-489173

    Enlarge

  4. dCas9-hHDAC3

    Plasmid
    #98591
    Purpose
    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.
    Depositor
    Zhaolan Zhou
    Article
    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10
    Insert
    dCAS9-HDAC3 (HDAC3 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    98591-plasmid-map-sequence-id-339879

    Enlarge

  5. SP6-VEE-GFP

    Plasmid
    #58976
    Purpose
    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Insert
    EGFP
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58976-plasmid-map-sequence-id-445055

    Enlarge

  8. BRD4(46-460)-HaloTag Fusion Vector

    Plasmid
    #238627
    Purpose
    Express BRD4(46-460)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    BRD4 (BRD4 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    (46-460)
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238627-plasmid-map-sequence-id-479503

    Enlarge

  10. pH2B-mNG2(11)

    Plasmid
    #206043
    Purpose
    Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.
    Depositor
    Alisa Piekny
    Article
    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7
    Insert
    H2B gene fused to the mNG2(11) fragment
    Use
    Synthetic Biology
    Tags
    H2B-mNG2(11)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    206043-plasmid-map-sequence-id-411120

    Enlarge

  13. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid
    #230003
    Purpose
    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230003-plasmid-map-sequence-id-457206

    Enlarge

  14. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Article
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112233-plasmid-map-sequence-id-213540

    Enlarge

  17. pICherryNeo

    Plasmid
    #52119
    Purpose
    a bicistronic IRES-mCherry containing vector for mammalian expression (CMV promoter)
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52119-plasmid-map-sequence-id-80505

    Enlarge

  26. Actin Binding Domain Tensionless Control

    Plasmid
    #215371
    Purpose
    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Insert
    Actin Binding Domain Tensionless Control
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215371-plasmid-map-sequence-id-430717

    Enlarge

  36. LD-B

    Plasmid
    #209861
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Tags
    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209861-plasmid-map-sequence-id-415472

    Enlarge

  38. HiBiT-TP53 Fusion Vector

    Plasmid
    #236885
    Purpose
    Express HiBiT-TP53 in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    TP53 (TP53 Human)
    Tags
    HiBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236885-plasmid-map-sequence-id-473395

    Enlarge

Showing: 3921 - 3960 of 80559 results