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Plasmid#197750PurposePhytobrick (MoClo) Level 0 PartDepositorInsertZmBbm-P2A-ZmWus2 bicistronic CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE792
Plasmid#167300PurposeVector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-tRNA2.0
Plasmid#158395PurposeGolden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D2.0
Plasmid#99906PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB101
Plasmid#68555PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNK6032
Plasmid#219738PurposeMoClo-compatible Level 0 - promoter + 5U vector carrying promoter FMVDepositorInsertFigwort mosaic virus (FMV) promoter
UseSynthetic BiologyTagsExpressionPlantMutationPromoterFMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGE1109
Plasmid#202773PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB116
Plasmid#68570PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMU-TGN41r
Plasmid#61176PurposeFluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under AtUBQ10 promoterDepositorInsertMtSYP41
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 hpIntron (GB1281)
Plasmid#160569PurposeIntron sequence from S. lycopersicumDepositorInserthpIntron
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB123 - pL0_rLUC-I (CDS1)
Plasmid#123181PurposeGolden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plantsDepositorInsertluciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
UseLuciferase; Part for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMU-NUCr
Plasmid#61168PurposeFluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoterDepositorInsertNLS-mCherry-GUS
UseTagsExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos
Plasmid#211840PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.DepositorInsertLRR-NEL
UseSynthetic BiologyTagsHA and mCherryExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlu/gRNA
Plasmid#59188PurposeServes as a shuttle vector for target oligo before insertion into Cas9 destination vectorDepositorInsertGuide RNA Cassette
UseCRISPRTagsExpressionPlantMutationPromoterU6 (Arabidopis)Available sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pE6c
Plasmid#17466Purposemodified pENTR vector for C-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJJB308
Plasmid#107699PurposeAtU6 Promoter-LwaCas13a-gRNA ScaffoldDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGW-TurboID-GOI-GFP
Plasmid#209383PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMU-LEr
Plasmid#61185PurposeFluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under AtUBQ10 promoterDepositorInsertMtRAB5A2
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCsA
Plasmid#136067PurposeAcceptor plasmid for even level position 1, lacZ blue-white screeningDepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEL032
Plasmid#137901PurposeSTU2.0 LbCas12a for plant genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE-35S-miniSdd7-nCas9-PBE-GmPPO2
Plasmid#204855PurposeFor cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI-mScarlet
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9Promoter35S, AtU6-26pAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21A
Plasmid#91128PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKI_ΩBBMVP16&WUS
Plasmid#220348PurposeExpresses BBM-VP16 and WUS in plant cellsDepositorUseTagsVP16 transcriptional activation domainExpressionPlantMutationPromoterCaMV 35S and RPS5AAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
lvl0 LhGR
Plasmid#153408PurposeDex inducible system trans activatorDepositorInsertLhGR CDS
UseTagsExpressionPlantMutationCodon optimizedPromoterAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCS104
Plasmid#209357PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal HaloTag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH-nCas9-PBE
Plasmid#98163PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-nCas9-UGI-NLS
UseRiceTagsExpressionPlantMutationPromoterUbiAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDL691
Plasmid#231166PurposeT-DNA encoding TRV2 with ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESSDepositorInsertipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
AL12R
Plasmid#161007PurposePP7-tagged reporter without a promoterDepositorTypeEmpty backboneUseTagsPP7 and H2B-mScarletExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_Venus_Hyg
Plasmid#159703PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsmVenusExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pChimera
Plasmid#61476PurposeVector for conventional cloning that contains AtU6-26 promoter and sgRNA backboneDepositorInsertU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE8
Plasmid#195569PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3DepositorInsertecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(gsagsaagsgef…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMU-ACTFr
Plasmid#61191PurposeFluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoterDepositorInsertAtFimbrin1-ABD
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
ePPE-spG
Plasmid#183096PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nspGCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpGCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOsU3
Plasmid#170132PurposeFor sgRNA expression in monocotyledons protoplastsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG302-SunTagng-22aa
Plasmid#115345PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genomeDepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPCTαKN0005
Plasmid#187569PurposeTranscriptional unit for constitutive expression of nanoluc luciferase driven by the 35s promoterDepositorInsertLuciferase
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE2
Plasmid#195368PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTENDepositorInserthA3A/Y130F-XTEN-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAS9-TPC
Plasmid#61478PurposeBinary expression vector with A.th. codon-optimized Cas9 for conventional cloningDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationPromoterPcUbi4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate 100RMOA mPing
Plasmid#196137PurposeMeasures mPing transposition in plantsDepositorInsertsPong TPase LA T2A ORF1SC1 ONE
mPing
UseBinaryTagsExpressionPlantMutationTPase NES sequence mutated L418A, L420A; T2A pept…Promoter35S and RPS5aAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145-ZmUbi-tRNA
Plasmid#158403PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_221
Plasmid#91115PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
L1_HyR-Ck1
Plasmid#136125PurposeL1 in position 1 (next to LB in L2 construct), for HygRDepositorInsertp5-35S:hptII_3t35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGWB511
Plasmid#74853PurposeGateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB215
Plasmid#68606PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-S11
Plasmid#108179PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB1alpha11:empty
Plasmid#106207PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only