4,297 results
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Plasmid#125808PurposeLevel0 xCas9 3.7DepositorInsertxCas9 3.7
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-ΔUGI
Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
UseTagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP107
Plasmid#207925PurposePremade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ289 (ErCas12a)
Plasmid#138126PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a without promoterDepositorInsertErCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aac.4
Plasmid#158406PurposeExpress single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
Plasmid#197966PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.DepositorInsertspco-vCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVp and pUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM159
Plasmid#84084Purposetarget miRNA159 for destruction in Arabidopsis thalianaDepositorInsertSTTM159
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAK-EL-02
Plasmid#125761PurposeEndlinker for tRNA-gRNA system when using only two guidesDepositorInsertEndlinker
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMY200
Plasmid#69946PurposeAgrobacterium-based transformation vectorDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ-dAaCas12b-Act3.0
Plasmid#158413PurposeCRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 MASp
Plasmid#153364PurposeAgrobacterium promoter for plant expressionDepositorInsertMAS promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINTBxb1
Plasmid#127536PurposePlasmid encodes A. thaliana codon optimized Integrase Bxb1.DepositorInsertIntegrase Bxb1 coding sequence codon optimized for A. thaliana expression.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PDF1::mCitrine-MBD
Plasmid#115222PurposeMicrotubule visualizationDepositorInsertMicrotubule binding domain
UseTagsmCitrineExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORFE0005
Plasmid#112073PurposeAtDeadCAS9 D10A H840A without stop codon module CDS1nsDepositorInsertAtDeadCAS9 D10A H840A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-35S-GW-Luc
Plasmid#80678Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterPro35SAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)
Plasmid#160622PurposeTU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLLDepositorInsertP35s_MS2:EDLL_Tnos
UseTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUM31dao
Plasmid#87242PurposePlastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plantsDepositorInsertD-amino oxidase
UseTagsExpressionPlantMutationPromoterplastid rrnAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGB Tnos:NptII:Pnos (GB0226)
Plasmid#68212PurposeTranscriptional unit for kanamycin resistance gene plant expressionDepositorInsertNptII
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P2_crU626
Plasmid#191777PurposeCas12a guide expression in dicotsDepositorInsertLbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P7_crU626
Plasmid#191782PurposeCas12a guide expression in dicotsDepositorInsertLbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB419
Plasmid#74813PurposeGateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21B
Plasmid#91129PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
SunTagCACTA1g2-22aa-TET1cd
Plasmid#106437PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoterDepositorInsertCACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB328
Plasmid#68656PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG20_MCS_blp
Plasmid#159710PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_MCS_Hyg
Plasmid#159709PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
hygII-UT-ABAleon2.15
Plasmid#106983PurposeABAleon2.15 in plant expression vector (Hygromycin resistance)DepositorInsertABAleon2.1
UseTagsExpressionPlantMutationPromoterUBQ10Available sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
BCJJ180
Plasmid#117516PurposeCas9_4 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_4:GCTT:BsaI
UseCRISPRTags3xFLAG and NLSExpressionPlantMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
lvl0 UBQ11p
Plasmid#153365PurposePlant promoterDepositorInsertUBQ11 promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
BCJJ339
Plasmid#117501PurposeRPS5a:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtRPS5aAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB522
Plasmid#74864PurposeGateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH49
Plasmid#128211Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-v2
Plasmid#138119PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoterDepositorInsertMb2Cas12a v2
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172R, N563R and K569RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
P366
Plasmid#139722PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, NpgaDepositorInsertpMOD_A''_pSlRBCS2:AsNpga:tAtuG7
UseLuciferaseTagsExpressionPlantMutationPromoterSlRBCS2Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUBN-paGFP-Dest
Plasmid#105108Purposefluorescent tagging in stably transformed plantsDepositorTypeEmpty backboneUseTagspaGFPExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941DR5-PacI-ath-STTM390
Plasmid#84117Purposetarget miRNA390 for destruction in Arabidopsis thalianaDepositorInsertSTTM390
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
JAB2603
Plasmid#190390PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD1:TM2-MAR
Plasmid#106214PurposeProvides N.tabacum TM2 matrix attachment region as a level 0 GoldenBraid partDepositorInsertN.tabacum TM2 matrix attachment region
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)13
Plasmid#127526PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGREAT18
Plasmid#170906PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBC1cer60G
Plasmid#161016Purposeubiquitously weakly expressed 60-meric GFP nanocageDepositorInsert60meric GFP nanocage
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
mUAV
Plasmid#153362PurposeConverts DNA sequences into PhytobricksDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB4068
Plasmid#187810PurposeModule for the constitutive expression of Cup2:Gal4AD and copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).DepositorInsertcopper-inducible dCasEV2.1
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [M1_n-1] (GB1210)
Plasmid#75411PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [M1_n-1]
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH56
Plasmid#125882PurposeLevel0 Rice OsACT1 Pol II promoterDepositorInsertLevel0 Rice OsAct1 Pol II promoter
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL12015
Plasmid#117506PurposeAtUBI10 promoter Golden Gate Level 0DepositorInsertBsaI:GGAG:pUBI10:AATG:BsaI
UseTagsExpressionPlantMutationPromoterAtUBI10Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBC1cer120G
Plasmid#161017Purposeubiquitously weakly expressed 120-meric GFP nanocageDepositorInsert120meric GFP nanocage
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 BlpR
Plasmid#153390PurposeConfers resistance to bialaphos or phosphinothricin (Glufosinate)DepositorInsertBlpR
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10::ZF2CACTA1_3xFLAG_TET1cd
Plasmid#106433PurposepUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsisDepositorInsertpUBQ10::ZF1CACTA1_3xFLAG_TET1cd (TET1 Mustard Weed, Human, Synthetic)
UseSynthetic BiologyTags3xFLAGExpressionPlantMutationPromoterAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only