4,545 results
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Plasmid#89269PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02DepositorInsertOsPDS-gRNA01 and OsPDS-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantPromoterMaize Ubiquitin1 promoterAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCHW00016
Plasmid#222447PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.DepositorInsertp65AD-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2-AA007
Plasmid#185750PurposeVector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
Tagsmembrane localization tag Lti6bExpressionPlantAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9-@GL1
Plasmid#86779PurposeDisruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9DepositorInsertsgRNA against GLABROUS1
UseSynthetic BiologyExpressionPlantPromoterArabidopsis U6-26 promoterAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGT5439
Plasmid#192351PurposeL0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stopDepositorInsertTTCG_Stop-tOCS_CGCT
ExpressionPlantAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEL029
Plasmid#137898PurposeSTU2.0 SpyCas9 RZ system for plant genome editingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-Cerulean-SKL
Plasmid#194048PurposeTransient expression of Cerulean-SKL in plant cell (Peroxisome)DepositorInsertCerulean
TagsSer-Lsy-LeuExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB205
Plasmid#68596PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTF-FLPe
Plasmid#139672PurposeExpression cassette for FLPe recombinase in T-DNA vectorDepositorInsertFLPe recombinase
ExpressionPlantPromoterMaize UbiquitinAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-PBE
Plasmid#98161PurposeExpresses CRISPR base editor in protoplastsDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
ExpressionPlantPromoterUbiAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Cas12j2
Plasmid#173926PurposeGateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMB-ACTLg
Plasmid#61195PurposeFluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoterDepositorInsertLifeAct-eGFP
TagseGFPExpressionPlantPromoterMtBCP1Available SinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFH47
Plasmid#125812PurposeLevel0 LbCas12a, human codon optimizedDepositorInsertLbCas12a, human codon optimized
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha2_SulR
Plasmid#186425Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoterDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia ā¦ExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPDK3182
Plasmid#97404Purposebinary construct of ER-targeted sfYFP1-10 for plant expressionDepositorInsertER-sfYFP1-10
TagsER targetExpressionPlantAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPDK3184
Plasmid#97405Purposebinary construct of cytosolic sfCFP1-10 tag for plant expressionDepositorInsertsfCFP1-10
TagscytosolExpressionPlantAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC-9-StUbi-11
Plasmid#197774PurposePhytobrick (MoClo) Level 0 PartDepositorInsertStUbi terminator
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PROM5_35Sx2
Plasmid#136112PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35Sx2 (CaMV)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_35S
Plasmid#136111PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35S (CaMV)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
lvl0 G7t
Plasmid#153380PurposeAgrobacterium terminator for plant expressionDepositorInsertG7 terminator
ExpressionPlantAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-GPXC71S
Plasmid#191760PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758DepositorInsertnuc-roGFP2-GPXC71S
UseBinary agrobacterium ti plasmidExpressionPlantMutationGPX has no peroxidatic Cysteine 71, which is replā¦PromoterCaMV 35SAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RVR
Plasmid#108861PurposeLbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutationsDepositorInsertLbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R, K554V and Y558R, Cpf1 is rice codon optimiā¦Available SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gma-pCAMBIA-1301-STTM171
Plasmid#84080PurposeDisturb the expression of miR171 in soybeanDepositorInsertmiR171
ExpressionBacterial, Plant, and Yeastā¦Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
PEP106E
Plasmid#97392Purposebinary construct of ER-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
TagsER targetExpressionPlantAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB427
Plasmid#68692PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10
Plasmid#197952PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.DepositorInsertspcoCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoterAtU6-26 and pUBQ10Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
lvl0 FAD2t
Plasmid#153377PurposePlant terminatorDepositorInsertFAD2 terminator
ExpressionPlantAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry cloā¦Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain formā¦Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
L1_KanR-Ck1
Plasmid#136123PurposeL1 in position 1 (next to LB in L2 construct), for KanRDepositorInsertp5-MpEF1a:nptII_3t35S
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk1_spacer
Plasmid#136071PurposeUse when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMpGWB227
Plasmid#68618PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB524
Plasmid#74866PurposeGateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB529
Plasmid#74871PurposeGateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239
Plasmid#108859PurposeFnCpf1 Gateway entry plasmidDepositorInsertFnCpf1
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationCpf1 is rice codon optimizedAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHub10-His-YFP-PIP2;1
Plasmid#99386Purposeoverexpression and isolation of recombinant At-PIP2;1 proteinDepositorAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDH
Plasmid#67584PurposeGene expression in plants. A promoter and/or reporter can be cloned into this plasmidDepositorTypeEmpty backboneExpressionPlantPromoterNoneAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
MIGS2.1 (FF570)
Plasmid#35247PurposeExpression of miR173 precursor under UBQ10 promoter; includes miR173 target site after 35S promoter (Basta selection marker)DepositorTypeEmpty backboneExpressionPlantPromoterCaMV 35SAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYL1300UaUf
Plasmid#183173PurposePlant binary vector with ccdB flanked by Ua and Uf unique nucleotide sequences.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1112
Plasmid#141066PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneTagsmScarlet-I and mVenusExpressionPlantPromoterCaMV 35S promoterAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRGEB-VirD2-Cas9
Plasmid#139803PurposeExpressing VirD-Cas9 fusion under ubiquitin promoterDepositorInsertVirD2-Cas9
TagsVirD2-Cas9ExpressionPlantPromoterUbiquitinAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL856
Plasmid#214256PurposeExpression TadDE-NG in rice cellsDepositorTypeEmpty backboneExpressionPlantAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-MpPHOT-Citrine
Plasmid#220106PurposeTransient expression of MpPHOT-Citrine in plant cellsDepositorInsertMpPHOT-Citrine
ExpressionPlantAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGEB34
Plasmid#158152PurposeConstruction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB443
Plasmid#74829PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:GFP-T35S
Plasmid#211836PurposePlant transient expression cassette for GFP (control) under UBQ10 promoter.DepositorInsertGFP control
UseSynthetic BiologyTagsGFPExpressionPlantPromoterUBIQUITIN10Available SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1267
Plasmid#141301PurposeGateway cloning compatible dual bioluminescent ratiometric binary vectorDepositorTypeEmpty backboneTagsgLUC and redLUCExpressionPlantPromoterCaMV 35S promoterAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL86900OD
Plasmid#86178PurposeGG compatible single transcript acceptor NPTIIDepositorTypeEmpty backboneExpressionPlantAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRATIO4214
Plasmid#141314PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneTagsmCerulean and mScarlet-IExpressionPlantPromoterUBQ10 promoterAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m3
Plasmid#149556PurposeGateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMsDepositorInsertecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
UseCRISPRExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domaiā¦Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133C2.0
Plasmid#99893PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU6Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only