4,625 results
-
Plasmid#61195PurposeFluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoterDepositorInsertLifeAct-eGFP
TagseGFPExpressionPlantPromoterMtBCP1Available SinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB524
Plasmid#74866PurposeGateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRGEB-VirD2-Cas9
Plasmid#139803PurposeExpressing VirD-Cas9 fusion under ubiquitin promoterDepositorInsertVirD2-Cas9
TagsVirD2-Cas9ExpressionPlantPromoterUbiquitinAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB427
Plasmid#68692PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha2_SulR
Plasmid#186425Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoterDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …ExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1112
Plasmid#141066PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneTagsmScarlet-I and mVenusExpressionPlantPromoterCaMV 35S promoterAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-GPXC71S
Plasmid#191760PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758DepositorInsertnuc-roGFP2-GPXC71S
UseBinary agrobacterium ti plasmidExpressionPlantMutationGPX has no peroxidatic Cysteine 71, which is repl…PromoterCaMV 35SAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB443
Plasmid#74829PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/Os-ubi:nnLuz-I{PIV-216}:NosT
Plasmid#212191PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the rice ubiquitin (R. ubi) promoter and UTRDepositorInsertnnLuz-I{PIV-216}
ExpressionPlantMutationPIV intron added at bp 216, eliminates expression…PromoterRice ubiquitinAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9-@GL1
Plasmid#86779PurposeDisruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9DepositorInsertsgRNA against GLABROUS1
UseSynthetic BiologyExpressionPlantPromoterArabidopsis U6-26 promoterAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYL1300UaUf
Plasmid#183173PurposePlant binary vector with ccdB flanked by Ua and Uf unique nucleotide sequences.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RVR
Plasmid#108861PurposeLbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutationsDepositorInsertLbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R, K554V and Y558R, Cpf1 is rice codon optimi…Available SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHW00016
Plasmid#222447PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.DepositorInsertp65AD-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGEB34
Plasmid#158152PurposeConstruction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGT5439
Plasmid#192351PurposeL0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stopDepositorInsertTTCG_Stop-tOCS_CGCT
ExpressionPlantAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHub10-His-YFP-PIP2;1
Plasmid#99386Purposeoverexpression and isolation of recombinant At-PIP2;1 proteinDepositorAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133C2.0
Plasmid#99893PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU6Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB227
Plasmid#68618PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH47
Plasmid#125812PurposeLevel0 LbCas12a, human codon optimizedDepositorInsertLbCas12a, human codon optimized
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAP L1A
Plasmid#153343PurposeMobius Assembly cloning and expression vector for plants with high-copy number origin of replicationDepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL86900OD
Plasmid#86178PurposeGG compatible single transcript acceptor NPTIIDepositorTypeEmpty backboneExpressionPlantAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC-9-StUbi-11
Plasmid#197774PurposePhytobrick (MoClo) Level 0 PartDepositorInsertStUbi terminator
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PROM5_35Sx2
Plasmid#136112PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35Sx2 (CaMV)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_35S
Plasmid#136111PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35S (CaMV)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMpGWB136
Plasmid#68590PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise
Plasmid#129650PurposeRieske FeS overexpressionDepositorInsertCDS of PetC gene encoding for the Rieske FeS subunit
ExpressionPlantMutationCodon modified for Golden GatePromoterpZmUbiAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1267
Plasmid#141301PurposeGateway cloning compatible dual bioluminescent ratiometric binary vectorDepositorTypeEmpty backboneTagsgLUC and redLUCExpressionPlantPromoterCaMV 35S promoterAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRATIO4214
Plasmid#141314PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneTagsmCerulean and mScarlet-IExpressionPlantPromoterUBQ10 promoterAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
AL13Rb-GAPC2
Plasmid#161009PurposePP7-tagged reporter driven by the Arabidopsis GAPC2 promoterDepositorInsertPP7 and H2B-mScarlet
ExpressionPlantAvailable SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10
Plasmid#197952PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.DepositorInsertspcoCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoterAtU6-26 and pUBQ10Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-SEIPIN1
Plasmid#96973PurposeExpress Arabidopsis SEIPIN1 gene in plants.DepositorAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
L1_KanR-Ck1
Plasmid#136123PurposeL1 in position 1 (next to LB in L2 construct), for KanRDepositorInsertp5-MpEF1a:nptII_3t35S
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk1_spacer
Plasmid#136071PurposeUse when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIAA5::LUC
Plasmid#141169PurposeLuminescent reporter for auxin signalingDepositorInsertAtIAA5 promoter
UseLuciferaseExpressionPlantPromoterAtIAA5, At1g15580Available SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMB-PMr
Plasmid#61181PurposeFluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under MtBCP1 promoterDepositorInsertAtPIP2a
TagsmCherryExpressionPlantPromoterMtBCP1Available SinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
PEP107E
Plasmid#97393Purposebinary construct of Golgi-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
TagsGolgi targetExpressionPlantAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE1c
Plasmid#17461Purposemodified pENTR vector for C-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEL856
Plasmid#214256PurposeExpression TadDE-NG in rice cellsDepositorTypeEmpty backboneExpressionPlantAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-MpPHOT-Citrine
Plasmid#220106PurposeTransient expression of MpPHOT-Citrine in plant cellsDepositorInsertMpPHOT-Citrine
ExpressionPlantAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only