4,457 results

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pECNUS_P6

Plasmid

#194348

Purpose

sgRNA position entry clone 6

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P7

Plasmid

#194349

Purpose

sgRNA position entry clone 7

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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BCJJ358

Plasmid

#117505

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtRPS5a

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB230

Plasmid

#68621

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

tdTomato

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCMB-AUTr

Plasmid

#61205

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTX198

Plasmid

#89262

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA01

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

Plasmid

#192380

Purpose

To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCO488

Plasmid

#139731

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Hisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_C'_CmYLCV:NnHisps:tHsp

Use

Luciferase

Expression

Plant

Promoter

CmYLCV

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM7547

Plasmid

#169091

Purpose

MoClo level 1 35S promoter-Db5GT-35S terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-Db5GT-35S terminator

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7523

Plasmid

#169090

Purpose

MoClo level 1 35S promoter-BvDODA1-Ocs terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvDODA1-Ocs terminator

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7535

Plasmid

#169089

Purpose

MoClo level 1 35S promoter-BvCYP76AD1-Nos terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvCYP76AD1-Nos terminator

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 NDUFA8t

Plasmid

#153379

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

NDUFA8 terminator

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PV225

Plasmid

#132334

Purpose

dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))

Tags

AT-CBF1

Expression

Plant

Mutation

Codon optimize for expression and stability in Ze…

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM828

Plasmid

#84157

Purpose

target miRNA828 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM828

Expression

Plant

Promoter

2x35S

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only
pTX172

Plasmid

#89259

Purpose

Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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p35S::mCh-HA-P2A-lifeact-mCh

Plasmid

#135234

Purpose

constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

mCh-HA-P2A-lifeact-mCh

Tags

HA, lifeact, and p2a

Expression

Plant

Promoter

35S

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFPL-Yh2

Plasmid

#61650

Purpose

Gateway vector for generating yellow fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced yellow fluorescent protein

Expression

Plant

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only
pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pG20_mCherry_Blp

Plasmid

#159702

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Tags

mCherry

Expression

Plant

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pNC-Cam2304-35S

Plasmid

#193385

Purpose

Nimble cloning-compatible vector for plant ectopic expression

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTT135

Plasmid

#207924

Purpose

Premade UBI10 driven expression of LhG4 (pUBQ10:GR-LhG4:tHSP18.2M) for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMP108

Plasmid

#207928

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M:FastRed, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBD001

Plasmid

#201330

Purpose

pDefence14:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)

Depositor

Benjamin Schwessinger

Insert

pDefence14:Eluc

Expression

Plant

Mutation

removal of BsaI and BpiI sites

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDIRECT_23D

Plasmid

#91141

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-1

Plasmid

#126053

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE671

Plasmid

#153237

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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L1_UBE2:mS-Lt-Ck4

Plasmid

#136132

Purpose

L1 in position 4, mark cell outline with mScarletI

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mScarletI-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_Nos

Plasmid

#136115

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_Nos (A. tumefaciens)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
BCJJ344

Plasmid

#117503

Purpose

UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtUBI10

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB3_Omega2_Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1678)

Plasmid

#160642

Purpose

Module for the dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

GRLacIBDGal4AD / RDF

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB527

Plasmid

#74869

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL1P6_crU626

Plasmid

#191781

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_crU626

Plasmid

#191778

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL1P4_crU626

Plasmid

#191779

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSW554 - pOp5:SP-mVenus-HDEL

Plasmid

#116006

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pZG71

Plasmid

#202577

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTX176

Plasmid

#89260

Purpose

Express estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

Plasmid

#192412

Purpose

To act as a 1-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

Plasmid

#192413

Purpose

To act as a 1-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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