4,307 results

1. pFH80

   Plasmid

   #128191

   Purpose

   Level1 ZmUBIp::xCas9 3.7:NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::xCas9 3.7:NOSt
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMpGWB332

   Plasmid

   #68660

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MpHSP17.8A1 promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ289-RVR

   Plasmid

   #138127

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   ErCas12a RVR variant
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   D513R, K519V and N523R

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TF101-STTM-miR156/157

   Plasmid

   #84811

   Purpose

   Turning off/down miR156/157 in Soybean

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM-miR156/157
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   unknown

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSS02:cyt-Peredox-mCherry

   Plasmid

   #161743

   Purpose

   cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

   Depositor

   Markus Schwarzländer

   Article

   Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

   Insert

   cyt-Peredox-mCherry
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin 10

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFLIPglu-3.2mDelta13

   Plasmid

   #12992

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A S112A Delta13
   

   Use

   Plant nanosensor

   Tags

   ECFP and EYFP

   Expression

   Plant

   Mutation

   F16A, S112A in binding protein

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pDest_pK7WG2_pRPS5A_CTP OleGFP

   Plasmid

   #171723

   Purpose

   ptpTALECD vector assembly, Destination vector;

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLXMA L2A

   Plasmid

   #153360

   Purpose

   Expression vector for plants with medium-copy number origin of replication

   Depositor

   Naomi Nakayama

   Article

   Andreou et al bioRxiv, March 31, 2021

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pICSL11023

   Plasmid

   #117542

   Purpose

   Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S_OMEGA(pICH51288)

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGB3omega1_KanR_BastaR

    Plasmid

    #186426

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMpGWB117

    Plasmid

    #68571

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lvl0 UBQ5t

    Plasmid

    #153383

    Purpose

    Plant terminator

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    UBQ5 terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre

    Plasmid

    #192421

    Purpose

    To act as the 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-loxP-OCS-FRT-loxP::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRATIO2151

    Plasmid

    #141300

    Purpose

    Gateway cloning compatible ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    Firefly Luciferase or LUC2 and mScarlet-I

    Expression

    Plant

    Mutation

    Promoter

    UBQ10 promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFGC5941-PacI-ath-STTM398

    Plasmid

    #84124

    Purpose

    target miRNA398 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM398
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFLIPglu-170nDelta13

    Plasmid

    #12989

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16F Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pTC208

    Plasmid

    #70017

    Purpose

    TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl Virus

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (TALENs) + Donor + GVR
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEE389

    Plasmid

    #149287

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

19. pTaCas9-D10A_5

    Plasmid

    #91170

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEE387

    Plasmid

    #149285

    Purpose

    T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_FT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGB 35s:dCas:EDLL:tNos (GB1190)

    Plasmid

    #75404

    Purpose

    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional Activator

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    dCas:EDLL
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTF101.3

    Plasmid

    #134772

    Purpose

    A binary plasmid containing double CaMV 35S promoter to drive bialophos resistance gene (bar) for plant transformation.

    Depositor

    Kan Wang

    Article

    Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGB3_alpha2 35:PhyB:VP16 (GB2745)

    Plasmid

    #160630

    Purpose

    TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

    Depositor

    Diego Orzaez

    Insert

    PhyBVP16
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pG20_Venus_Blp

    Plasmid

    #159704

    Purpose

    pGREEN-based cloning construct for expression in planta

    Depositor

    Hans-Henning Kunz

    Article

    Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

    Type

    Empty backbone

    Use

    Tags

    mVenus

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMpGWB415

    Plasmid

    #68680

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Nuclear localization signal-tagged Citrine (Citri…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBGC155-MXMT

    Plasmid

    #79818

    Purpose

    GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

    Depositor

    Masamitsu Wada, Yutaka Kodama

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Insert

    CaMXMT1
    

    Use

    Reporter plasmid

    Tags

    GC155/GFP (155–239)

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter and CaMV35S

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBGN155

    Plasmid

    #74992

    Purpose

    GFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plants

    Depositor

    Masamitsu Wada, Yutaka Kodama

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Type

    Empty backbone

    Use

    Reporter plasmid

    Tags

    GFP (1–155)/GN155

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEL080

    Plasmid

    #137889

    Purpose

    iSTU-CRISPR/Cas9 with OsCDPK2 intron, NU structure backbone

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEL085

    Plasmid

    #137893

    Purpose

    iSTU-CRISPR/Cas9 with RcCat intron, tRNA structure backbone

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEL083

    Plasmid

    #137892

    Purpose

    iSTU-CRISPR/Cas9 with RcCat intron, NU structure backbone

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMpGWB102-Citrine-NLS

    Plasmid

    #79136

    Purpose

    Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants

    Depositor

    Yutaka Kodama

    Article

    Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

    Insert

    Citrine-NLS
    

    Use

    Tags

    NLS (Nuclear Localization Signal)

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFGC5941-PacI-ath-STTM395

    Plasmid

    #84121

    Purpose

    target miRNA395 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM395
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMpGWB403-Lifeact-Citrine

    Plasmid

    #100582

    Purpose

    Binary vector for expression of Citrine-fused Lifeact (Lifeact-Citrine) to visualize actin filaments in plants

    Depositor

    Yutaka Kodama

    Article

    Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

    Insert

    Lifeact-Citrine
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBRN168

    Plasmid

    #79668

    Purpose

    DsREDmonomer-based BiFC vector for transient expression of RN (1-168) in plants.

    Depositor

    Masamitsu Wada, Yutaka Kodama

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Type

    Empty backbone

    Use

    Reporter plasmid

    Tags

    DsRED-monomer (1–168)/RN168

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFGC5941-PacI-ath-STTM408

    Plasmid

    #84132

    Purpose

    target miRNA408 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM408
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYPQ292-D570A

    Plasmid

    #129679

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    D570A; AaCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJG488

    Plasmid

    #91196

    Purpose

    WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nTaCas9_H840A+gUbi8+gUbi1+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MIGS3.1 (FF537)

    Plasmid

    #35249

    Depositor

    Detlef Weigel

    Article

    de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFGC5941-PacI-ath-STTM773a/b

    Plasmid

    #84141

    Purpose

    target miRNA773a/b for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM773a/b
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDGE4

    Plasmid

    #79443

    Purpose

    recipient plasmid [multiplexing]; pPcUbi:Cas9, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge