1,738 results

1. L3790

   Plasmid

   #1596

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   Tags

   myo2-1090, myo25', IVSA, rf2128, T7p, gfpN, …

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMB67

   Plasmid

   #47947

   Purpose

   Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and egl-13 NLS

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.48

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SREC 2: abu-1::abu-1::gfp

   Plasmid

   #21825

   Depositor

   David Ron

   Article

   Urano et al J Cell Biol. 2002 Aug 19. 158(4):639-46.

   Insert

   abu-1 promoter and coding region (abu-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCM5.32

   Plasmid

   #19397

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Sep 23. ():.

   Insert

   mes-2 3'utr in pDONRP2RP3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

   Plasmid

   #159846

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJH4330

   Plasmid

   #191700

   Purpose

   Punc-17::GtACR2::wCherry unc-54 3' UTR C.elegans neuron expression of GtACR2 RFP

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   GtACR2
   

   Use

   Tags

   wCherry

   Expression

   Worm

   Mutation

   Promoter

   Punc-17

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDD372

   Plasmid

   #91824

   Purpose

   Codon-optimized GFP donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   GFP-C1
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGC94

   Plasmid

   #19610

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Phsp-16.2::FLP::let-858(3’)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

   Plasmid

   #159866

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMB63

    Plasmid

    #47945

    Purpose

    Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and egl-13 NLS

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

    Plasmid

    #107137

    Purpose

    Dominant negative PKA cGAL effector for C. elegans

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    kin-2a(G310D)::SL2::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    15xUAS-delta-pes-10(basal promoter)

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH4329

    Plasmid

    #191679

    Purpose

    Prgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GtACR2
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

    Plasmid

    #182355

    Purpose

    mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pdestDD03

    Plasmid

    #26414

    Depositor

    Marc Vidal

    Article

    Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

    Insert

    attR4-ccdB-attR2 cassette
    

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

    Plasmid

    #159874

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSAH61 - [ASELp | wrmScarlet | tbb-2 UTR]

    Plasmid

    #200335

    Purpose

    mScarlet BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

    Depositor

    Christian Froekjaer-Jensen

    Insert

    [ASELp | wrmScarlet | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    WBGene00001534, gcy-7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDpy-7::oxCerulean-oxVenus::Lgg-1

    Plasmid

    #68042

    Purpose

    Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

    Depositor

    Dana Miller

    Article

    Chapin et al Aging (Albany NY). 2015 Jun 30.

    Insert

    pDpy-7::oxCerulean-oxVenus::Lgg-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TU#710

    Plasmid

    #15278

    Depositor

    Martin Chalfie

    Article

    Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

    Insert

    nzgfp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKS2236

    Plasmid

    #37833

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    YFP
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    nhx-2

    Available since

    Jan. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. rgef-1p::NmBirA

    Plasmid

    #79971

    Purpose

    encodes E.coli biotin-ligase under neuronal promotor for C.elegans

    Depositor

    Baris Tursun

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    BirA
    

    Use

    Tags

    NLS::myc

    Expression

    Worm

    Mutation

    Promoter

    rgef

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDD377

    Plasmid

    #91823

    Purpose

    Codon-optimized mTurquoise2 donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    mTurquoise2-C1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMB71

    Plasmid

    #73085

    Purpose

    Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    BirA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSR33

    Plasmid

    #69254

    Purpose

    phsp16.2::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hsp16.2

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. L3691

    Plasmid

    #1587

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    mec7-836, mec7Tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMOD4 GFP

    Plasmid

    #19181

    Depositor

    Al Fisher

    Article

    Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

    Insert

    Green fluorescent protein
    

    Use

    Tags

    FLAG and daf-12 N-term 328 amino acids

    Expression

    Worm

    Mutation

    S65C, contains synthetic introns for C. elegans

    Promoter

    Available since

    Jan. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCM3.19

    Plasmid

    #19401

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Sep 23. ():.

    Insert

    tbb-2 ORF+3' in pDONR201 (tbb-2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBN312

    Plasmid

    #86734

    Purpose

    GFP with Frt sites for FLP-mediated knock-out of gene function in C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    GFP_Frt_KO
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. B1979

    Plasmid

    #59181

    Purpose

    Expresses optimized blasticidin S resistance gene (BSD) for C. elegans

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    Blasticidin S resistance
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rpl-28

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

    Plasmid

    #182347

    Purpose

    GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159869

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge