1,755 results

1. pGC680

   Plasmid

   #107018

   Purpose

   GFP reporter of lag-2 driven by 3 kb promoter

   Depositor

   Jane Hubbard

   Article

   Pekar et al Development. 2017 Aug 15;144(16):2896-29

   Insert

   GFP-PH
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   lag-2

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB67

   Plasmid

   #47947

   Purpose

   Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and egl-13 NLS

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.48

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L3790

   Plasmid

   #1596

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   Tags

   myo2-1090, myo25', IVSA, rf2128, T7p, gfpN, …

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJRM3 - [BAGp | mScarlet | tbb-2 UTR]

   Plasmid

   #204616

   Purpose

   mScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

   Depositor

   Christian Froekjaer-Jensen

   Article

   AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

   Insert

   [BAGp | wrmScarlet | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJRM4 - [BAGp | gfp | tbb-2 UTR]

   Plasmid

   #204617

   Purpose

   gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

   Depositor

   Christian Froekjaer-Jensen

   Article

   AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

   Insert

   [BAGp | gfp | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MKV937/nlp-1p::FlincG3

   Plasmid

   #140508

   Purpose

   This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

   Depositor

   Miri VanHoven

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   FlincG3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   nlp-1

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL1121

   Plasmid

   #78188

   Purpose

   intestinal GFP expression by fasting in worms

   Depositor

   Junho Lee

   Article

   Jo et al Cell Metab. 2009 May;9(5):440-8. doi: 10

   Insert

   fil-2 (fil-2 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   fil-2

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

   Plasmid

   #159846

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMB63

   Plasmid

   #47945

   Purpose

   Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and egl-13 NLS

   Expression

   Worm

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDD372

    Plasmid

    #91824

    Purpose

    Codon-optimized GFP donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    GFP-C1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJH4260

    Plasmid

    #191678

    Purpose

    Ptwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarlet

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP6s
    

    Use

    Tags

    wScarlet

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40

    Available since

    Nov. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGC94

    Plasmid

    #19610

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Phsp-16.2::FLP::let-858(3’)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pdestDD03

    Plasmid

    #26414

    Depositor

    Marc Vidal

    Article

    Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

    Insert

    attR4-ccdB-attR2 cassette
    

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

    Plasmid

    #159866

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TU#710

    Plasmid

    #15278

    Depositor

    Martin Chalfie

    Article

    Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

    Insert

    nzgfp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SREC 2: abu-1::abu-1::gfp

    Plasmid

    #21825

    Depositor

    David Ron

    Article

    Urano et al J Cell Biol. 2002 Aug 19. 158(4):639-46.

    Insert

    abu-1 promoter and coding region (abu-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH4330

    Plasmid

    #191700

    Purpose

    Punc-17::GtACR2::wCherry unc-54 3' UTR C.elegans neuron expression of GtACR2 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GtACR2
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-17

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

    Plasmid

    #182355

    Purpose

    mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

    Plasmid

    #159874

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

    Plasmid

    #107137

    Purpose

    Dominant negative PKA cGAL effector for C. elegans

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    kin-2a(G310D)::SL2::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    15xUAS-delta-pes-10(basal promoter)

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCM5.32

    Plasmid

    #19397

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    mes-2 3'utr in pDONRP2RP3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDpy-7::oxCerulean-oxVenus::Lgg-1

    Plasmid

    #68042

    Purpose

    Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

    Depositor

    Dana Miller

    Article

    Chapin et al Aging (Albany NY). 2015 Jun 30.

    Insert

    pDpy-7::oxCerulean-oxVenus::Lgg-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJH4329

    Plasmid

    #191679

    Purpose

    Prgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GtACR2
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKS2236

    Plasmid

    #37833

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    YFP
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    nhx-2

    Available since

    Jan. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. rgef-1p::NmBirA

    Plasmid

    #79971

    Purpose

    encodes E.coli biotin-ligase under neuronal promotor for C.elegans

    Depositor

    Baris Tursun

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    BirA
    

    Use

    Tags

    NLS::myc

    Expression

    Worm

    Mutation

    Promoter

    rgef

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMB71

    Plasmid

    #73085

    Purpose

    Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    BirA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDD377

    Plasmid

    #91823

    Purpose

    Codon-optimized mTurquoise2 donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    mTurquoise2-C1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. L3691

    Plasmid

    #1587

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    mec7-836, mec7Tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSR33

    Plasmid

    #69254

    Purpose

    phsp16.2::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hsp16.2

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. B1979

    Plasmid

    #59181

    Purpose

    Expresses optimized blasticidin S resistance gene (BSD) for C. elegans

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    Blasticidin S resistance
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rpl-28

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge