1,774 results

1. pSEM254 - sgRNA mosTI neoR

   Plasmid

   #159831

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI neoR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEM322 - array co-injection fragment mosTI neoR

   Plasmid

   #159832

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   array co-injection fragment mosTI neoR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCG150_double_phiC31_attB

   Plasmid

   #169693

   Purpose

   cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

   Depositor

   Shih-Peng Chan

   Article

   Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

   Plasmid

   #158967

   Purpose

   Ubiquitous expression of mtON in C. elegans

   Depositor

   Andrew Wojtovich

   Article

   Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

   Insert

   eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   eft-3

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMDJ231 - Phsp Cas9

   Plasmid

   #191382

   Purpose

   Heat shock inducible Cas9 expression in C. elegans.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Phsp-16.41 Cas9 gpd-2 tagRFP-t
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Phsp-16.41

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD268

   Plasmid

   #132523

   Purpose

   mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein, Daniel Dickinson

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mNG-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized mNG

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTN14

   Plasmid

   #178945

   Purpose

   Expresses AtTIR1(F79G) in C elegans

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Insert

   AtTIR1(F74G)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSEM246 - mosTI - unc-119 - MCS

   Plasmid

   #182343

   Purpose

   Empty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: tgcc - MCS - gagc)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJR031

   Plasmid

   #198825

   Purpose

   Stable step function ChR2 variant. Activated by bluelight, terminated by green light.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

    Plasmid

    #159807

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLF3FShC

    Plasmid

    #153083

    Purpose

    RMCE integration vector with self excising cassette

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ150 - pDESTttTi5605[R4-R3]

    Plasmid

    #19329

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    pDESTttTi5605[R4-R3]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNMSB104

    Plasmid

    #199314

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, C. elegans codon optimized …

    Promoter

    flp-18

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF11-wmiRFP713-T2A-HO1

    Plasmid

    #197246

    Purpose

    Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP713-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPK605

    Plasmid

    #38148

    Depositor

    Patricia Kuwabara

    Inserts

    unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR) (unc-119 Nematode)
    pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    pie-1 promoter and unc-119

    Available Since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSF143-wArchon1-KGC-EGFP-ER2

    Plasmid

    #108429

    Purpose

    Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    wArchon1-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    flp-18

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSF11-wElectra2

    Plasmid

    #184933

    Purpose

    Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    tag-168

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSF11-wmiRFP680-T2A-HO1

    Plasmid

    #197244

    Purpose

    Expresses the protein of wmiRFP680-T2A-HO1 in neurons of C elegan

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP680-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJW2098

    Plasmid

    #163094

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

    Plasmid

    #159899

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge