1,755 results

1. pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

   Plasmid

   #159899

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | tagRFP | cbr-tbb-2 UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF11-wmTagBFP2

   Plasmid

   #184934

   Purpose

   Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   tag-168

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGK-EGFP-FKBPF36V

   Plasmid

   #199765

   Purpose

   plasmid for targeting dTAG into the AAVS1 locus

   Depositor

   Hannes Lans

   Article

   van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

   Insert

   GFP-dTAG
   

   Use

   Tags

   GFP and dTAG

   Expression

   Mammalian and Worm

   Mutation

   Promoter

   hPGK

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGH8 - pRAB-3::mCherry::unc-54utr

   Plasmid

   #19359

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Prab-3::mCherry
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdestDD04Neo

   Plasmid

   #26386

   Depositor

   Denis Dupuy

   Article

   Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

   Plasmid

   #71719

   Purpose

   Expressing a GFP-tagged degron in the soma of C. elegans

   Depositor

   Abby Dernburg

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   auxin-responsive protein IAA17 (AXR3 Mustard Weed)
   

   Use

   Tags

   EmGFP

   Expression

   Worm

   Mutation

   minimal degron sequence (71-114aa) with start cod…

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEZ-1

   Plasmid

   #51868

   Purpose

   Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

   Depositor

   Meng-Qiu Dong

   Article

   Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

   Insert

   psdhb-1::mtLS-cpYFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   sdhb-1

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD104 (Peft-3::Cre)

   Plasmid

   #47551

   Purpose

   Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Insert

   Cre Recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIP_RNA1

   Plasmid

   #61259

   Purpose

   Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

   Depositor

   David Wang

   Article

   Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

   Insert

   Orsay RNA1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   heat shock promoter

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHIP_RNA2FLAG

    Plasmid

    #61261

    Purpose

    Flag tagged Orsay virus RNA2 genome segment expression plasmid

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA2
    

    Use

    Tags

    Flag fused

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD287 (mKate2^SEC^3xMyc

    Plasmid

    #70685

    Purpose

    mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized mKate2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid

    #154840

    Purpose

    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    HYGR∆ encodes aa 1-226

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. T444T-GFP(NCO)

    Plasmid

    #183972

    Purpose

    Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP

    Depositor

    David Matus

    Article

    Palmisano et al CourseSource, 2022

    Insert

    GFP (non-codon-optimized)
    

    Use

    RNAi

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHygR2r

    Plasmid

    #200072

    Purpose

    hygR selection nls-mScarlet marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHygG2r

    Plasmid

    #200069

    Purpose

    hygR selection nls-mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBN42

    Plasmid

    #86713

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

    Insert

    Pmyo-3::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW1659

    Plasmid

    #154314

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::AID*::3xFLAG for NT slot
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ2369 - intron GG1 - 250bp

    Plasmid

    #159877

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG1 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEY14

    Plasmid

    #191059

    Purpose

    acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-5(orf) (acr-5 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1821

    Plasmid

    #163092

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mScarlet^SEC (Lox511I)^::3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge