1,791 results

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pCFJ1662

Plasmid

#51482

Purpose

miniMos transposable element with HygroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pJW2098

Plasmid

#163094

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJM31

Plasmid

#52986

Purpose

A secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensor

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Annexin V (ANXA5 Human)

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Promoter

hsp-16.41

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSEM318 - sgRNA mosTI unc-119 single-copy

Plasmid

#159822

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 single-copy

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1415

Plasmid

#84828

Purpose

Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns

Depositor

Andrew Fire

Article

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

C. elegans codon optimized GFP

Expression

Bacterial and Worm

Promoter

Peft-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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XW09

Plasmid

#65833

Purpose

Punc-4c-QS-SL2-mcherry3'UTR-unc-54

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Tags

mCherry

Expression

Worm

Promoter

Punc-4c

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRB1080

Plasmid

#71309

Purpose

Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VQR Cas9 variant

Use

CRISPR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Pflp-18::NGL-1::spGFP11

Plasmid

#65828

Purpose

For trans-synaptic labeling

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

NLG-1 (nlg-1 Nematode)

Tags

spGFP11

Expression

Worm

Mutation

N142S

Promoter

Pflp-18

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1259

Plasmid

#61251

Purpose

Cas9 plasmid with sgRNA deleted; for use in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

Deletion

Use

CRISPR

Expression

Worm

Mutation

Deleted sgRNA(F+E) sequence from pJW1219

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Promoter

myo-3 and unc-119

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pBCN27-Pmyo-2::GFP::unc-54_3'UTR

Plasmid

#26347

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Insert

Pmyo-2::GFP::unc-54_3'UTR

Expression

Worm

Available Since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pRG5273Neo

Plasmid

#26388

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available Since

Oct. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Plasmid

#158967

Purpose

Ubiquitous expression of mtON in C. elegans

Depositor

Andrew Wojtovich

Article

Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

Insert

eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Tags

GFP

Expression

Worm

Promoter

eft-3

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJW2086

Plasmid

#154335

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLF3FShC

Plasmid

#153083

Purpose

RMCE integration vector with self excising cassette

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1955

Plasmid

#84830

Purpose

Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

Depositor

Andrew Fire

Article

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

C. elegans codon optimized tdTomato

Expression

Bacterial and Worm

Promoter

Peft-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBCN21-R4R3

Plasmid

#26301

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Type

Empty backbone

Expression

Worm

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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TU#711

Plasmid

#15279

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czgfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pDD268

Plasmid

#132523

Purpose

mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Daniel Dickinson, Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mNG-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized mNG

Expression

Worm

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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TC358

Plasmid

#122613

Purpose

TRE-delta-pes-10-GFP

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

7XTRE-delta-pes-10-GFP

Expression

Worm

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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