1,755 results

1. pPD95.75-wVenus

   Plasmid

   #37464

   Depositor

   Hitoshi Sawa

   Article

   Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   none

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR

   Plasmid

   #225924

   Purpose

   Expressing LGC-47 in AVA neuron

   Depositor

   Andrew Leifer

   Article

   Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

   Insert

   LGC-47 (lgc-47 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rig-3

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDACR3882 [rab-3p::HYlight::let-858utr]

   Plasmid

   #207666

   Purpose

   Expresses worm-optimized HYlight in C. elegans neurons

   Depositor

   Daniel Colón-Ramos

   Article

   Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   HYlight Biosensor
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDACR3883 [rab-3p::HYlight-RA::let-858utr]

   Plasmid

   #207667

   Purpose

   Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

   Depositor

   Daniel Colón-Ramos

   Article

   Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   HYlight-RA [T152E]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJR014

   Plasmid

   #198813

   Purpose

   Natural light-gated anion channels. In vivo inhibition of neurons using blue light.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GtACR2::SL2::GFP::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS178

   Plasmid

   #215678

   Purpose

   Empty repair plasmid for ChrII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + loxP + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Promoter

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS114

   Plasmid

   #215677

   Purpose

   Empty repair plasmid for ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + loxN + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW2171

   Plasmid

   #163095

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xHA-miniTurbo_pAS32

   Plasmid

   #118222

   Purpose

   expresses 3xHA-tagged miniTurbo in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   ges-1p

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSH69

    Plasmid

    #127702

    Purpose

    Prab-3::ssGFP1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    ssGFP-1/10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCZGY405

    Plasmid

    #172694

    Purpose

    Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    rab-3 (rab-3 Nematode)
    

    Use

    Tags

    Punc-25 and mcherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJW6.01

    Plasmid

    #62882

    Purpose

    Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    GFP::MEG-3
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    meg-3

    Available since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ1320

    Plasmid

    #84829

    Purpose

    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized GFP
    

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Peft-3

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPD95_25

    Plasmid

    #1486

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    IVSA, ATG-SecSig, pes10

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXF121

    Plasmid

    #139028

    Purpose

    MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    short mex-5 promoter (mex-5 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEY41

    Plasmid

    #191045

    Purpose

    dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    dat-1 (dat-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEY43

    Plasmid

    #191047

    Purpose

    eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    eat-4(prom6-1) (eat-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pJJR83

    Plasmid

    #75028

    Purpose

    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    mCherry^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge