7,942 results

1. pABY-T3

   Plasmid

   #201766

   Purpose

   For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   ALFA tag
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pABY-T4

   Plasmid

   #201767

   Purpose

   For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   ALFA tag
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWS931

   Plasmid

   #123053

   Purpose

   TetR bacterial repressor

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   TetR-NLS [3a]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG416GAL-EGFP

   Plasmid

   #166140

   Purpose

   EGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. YN2_1_LT58_X2site

   Plasmid

   #177705

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pILGFP5EA4

   Plasmid

   #185879

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LT1_36_QUEEN-2m

   Plasmid

   #177708

   Purpose

   Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   pTEF1-QUEEN2m
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pABY-c45

   Plasmid

   #201771

   Purpose

   Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   NbALFA
   

   Use

   Tags

   40aa Linker, mNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire

   Plasmid

   #203655

   Purpose

   Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Tags

   Sapphire

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LT1_33_pTEFmut8-mCherry

    Plasmid

    #177706

    Purpose

    Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEFmut8-mCherry
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBHM2404

    Plasmid

    #173441

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-NAT1
    

    Use

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAS9id

    Plasmid

    #141251

    Purpose

    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB2793

    Plasmid

    #64043

    Purpose

    pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter and none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAPZa-STE2-1D4

    Plasmid

    #133422

    Purpose

    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S, C252A

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBHM2406

    Plasmid

    #173442

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-HYGB
    

    Use

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HO-hisG-URA3-hisG-poly-HO

    Plasmid

    #51663

    Purpose

    yeast plasmid for integration of target sequence at HO locus, URA3 selection

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFG2

    Plasmid

    #87250

    Purpose

    gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Nat
    

    Use

    Tags

    parS segments

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCT302

    Plasmid

    #41845

    Depositor

    Dane Wittrup

    Article

    Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

    Insert

    4-4-20
    

    Use

    Tags

    HA and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. p406GAL1

    Plasmid

    #17437

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GAL1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge