8,025 results

1. pAG416GAL-EGFP

   Plasmid

   #166140

   Purpose

   EGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG303-Gal-PR50

   Plasmid

   #84905

   Purpose

   induce expression of dipeptide repeat PR in yeast

   Depositor

   Aaron Gitler

   Article

   Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

   Insert

   PR50 (C9orf72 Synthetic)
   

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS314-T7-GFP

   Plasmid

   #33130

   Depositor

   Michael Rosbash

   Article

   Dower et al RNA. 2002 May;8(5):686-97.

   Insert

   GFP with TDH3 5' and 3' UTR (gfp )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSC5GGv1

   Plasmid

   #194645

   Purpose

   pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGS_128

   Plasmid

   #160651

   Purpose

   Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e3 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e3 allele

   Promoter

   pZ estradiol inducible promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDRF1 mBaojin-T2A-mCherry

   Plasmid

   #219842

   Purpose

   equimolar expression of mBaojin and mCherry in budding yeast

   Depositor

   Bas Teusink

   Insert

   mBaojin-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGK401red-­rtetTAK8N, L131L

   Plasmid

   #165968

   Purpose

   Expresses rTetTA (K8N, L131L) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   tetR
   

   Use

   Synthetic Biology

   Tags

   three copies of VP16 (VP48)

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

   Plasmid

   #69100

   Purpose

   URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

   Depositor

   David Botstein

   Inserts

   yZ3EV cassette
   Z3 promoter
   yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB3cH_pGAP_23*_pPFK300_Cas9

   Plasmid

   #104912

   Purpose

   hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. YIplac211-SEC7-msCFPx6

    Plasmid

    #105268

    Purpose

    Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Sec7 portion (SEC7 Budding Yeast)
    

    Use

    Tags

    msCFPx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. HO-Poly-KanMX4-HO

    Plasmid

    #51662

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRG226

    Plasmid

    #64529

    Purpose

    Episomal E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid

    #221158

    Purpose

    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Use

    Tags

    mGFP

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid

    #221159

    Purpose

    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Use

    Tags

    mScarletI

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG426GAL-ccdB-EGFP

    Plasmid

    #14203

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFTK055

    Plasmid

    #171327

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    rTA2S-M2 (TetR-3xVP16) transcriptional activator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire

    Plasmid

    #203655

    Purpose

    Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 2015-N iFAST

    Plasmid

    #209415

    Purpose

    iFAST expression plasmid using MET3p

    Depositor

    Patrick Van Dijck

    Article

    Devos et al Microbiology (Reading). 2024 Mar;170(3).

    Insert

    iFAST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET3

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSc-humanADAR2

    Plasmid

    #171106

    Purpose

    Expresses human ADAR2 in yeast.

    Depositor

    Brenda Bass

    Article

    Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 1

    Insert

    human ADAR2 (ADARB1 Human)
    

    Use

    Tags

    His12_TEV

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXP220

    Plasmid

    #26832

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCTCON2-GFP

    Plasmid

    #158130

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. p404TEF1

    Plasmid

    #15972

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    TEF1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104910

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pILGFP3AG4

    Plasmid

    #185874

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFR

    Plasmid

    #24585

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNTI661 pRPR1(TetO)-sgRNA

    Plasmid

    #139475

    Purpose

    Vector for tet-regulated sgRNA expression in yeast

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Insert

    single guide RNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pRPR1(TetO)

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSS146

    Plasmid

    #64770

    Purpose

    For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Use

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Mutation

    Promoter

    TDH4

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. bRA89

    Plasmid

    #100950

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGWKS9

    Plasmid

    #139416

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge