7,933 results

1. pPAP004

   Plasmid

   #160911

   Purpose

   E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

   Insert

   lacZ selection cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ZJOM151

   Plasmid

   #133643

   Purpose

   Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-COX4-GFP (COX4 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPOM033_P7_3"His5

   Plasmid

   #216458

   Purpose

   3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   3''his5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPOM026_P2_pNMT1

   Plasmid

   #216451

   Purpose

   Regulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter nmt1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPOM019_P2_pRPL2102

   Plasmid

   #216444

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter rpl2102
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPOM015_P2_pADH1

   Plasmid

   #216440

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter adh1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPOM013_P2_pENO101

   Plasmid

   #216438

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter eno101
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCEV-G2-Km ymScarletI

   Plasmid

   #193960

   Purpose

   TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast

   Depositor

   Bas Teusink

   Insert

   ymScarletI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YIplac211-CHS3-msGFPx3

   Plasmid

   #105257

   Purpose

   Plasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Chs3 portion (CHS3 Budding Yeast)
   

   Use

   Tags

   msGFPx3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ZJOM20

    Plasmid

    #133644

    Purpose

    Integration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-PEX1-2GFP (PEX1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJZC533

    Plasmid

    #135435

    Purpose

    Yeast integrating plasmid for pTEF1-mCherry reporter gene

    Depositor

    Jesse Zalatan

    Article

    Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    pTEF1

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYZ183

    Plasmid

    #98425

    Purpose

    Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYZ184

    Plasmid

    #98426

    Purpose

    Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DdMCU

    Plasmid

    #67642

    Purpose

    DdMCU-Flag in pACT2 vector human codon optimised

    Depositor

    Vamsi Mootha

    Article

    Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    DdMCU
    

    Use

    Tags

    Flag tag with linker

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MAP72_MS2

    Plasmid

    #220623

    Purpose

    Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)

    Plasmid

    #86416

    Purpose

    ONM/ER split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP11-mCherry-Scs2TM
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZJOM153

    Plasmid

    #133642

    Purpose

    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    Vph1-2GFP (VPH1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS315e_pGal-nCas9(D10A)-PmCDA1

    Plasmid

    #79617

    Purpose

    Expresses nCas9(D10A)-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG413GPD-ccdB

    Plasmid

    #14142

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSAC200

    Plasmid

    #187911

    Purpose

    psac200-crispey-bar-vector

    Depositor

    Hunter Fraser

    Article

    Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSAC212

    Plasmid

    #187912

    Purpose

    psac212-crispey-bar-ade2KO

    Depositor

    Hunter Fraser

    Article

    Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

    Insert

    ade2 KO CRISPEY cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYZ157

    Plasmid

    #98413

    Purpose

    Pombe Expression Vector - nmt81 promoter with Sp.ura4 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pROS10

    Plasmid

    #107924

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHES835

    Plasmid

    #87946

    Purpose

    Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-YFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJAID5XY

    Plasmid

    #165051

    Purpose

    Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYX233-DHFR

    Plasmid

    #163761

    Purpose

    Gal-inducible yeast expression of cytosolic DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    DHFR (Dhfr Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFA6a-mRFP-ura4MX6

    Plasmid

    #49183

    Purpose

    genomic mRFP tagging in S. pombe

    Depositor

    Eishi Noguchi

    Article

    Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

    Type

    Empty backbone

    Use

    Tags

    mRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS403-PGAL1-hpSC_URA3

    Plasmid

    #22317

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Sep 10. ():.

    Insert

    URA hairpin
    

    Use

    Integrating plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUNIV-EGFP

    Plasmid

    #24706

    Depositor

    Cynthia Czajkowski

    Article

    Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

    Insert

    EGFP (eGFP Aequoria victoria)
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ZJOM160

    Plasmid

    #133670

    Purpose

    Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-mTagBFP2-SKL
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p404-BrdU-Inc

    Plasmid

    #71790

    Purpose

    BrdU-incorporating plasmid with selectable marker TRP1

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid

    #181724

    Purpose

    Galactose inducible expression of Alpha-synuclein-H50Q

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H50Q

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS406 ATG9 stopGFP

    Plasmid

    #114669

    Purpose

    Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

    Insert

    ATG9
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Changed the three linker base pairs after the las…

    Promoter

    ATG9

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ZJOM31

    Plasmid

    #133645

    Purpose

    Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-TGL3-GFP (TGL3 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAV103

    Plasmid

    #63193

    Purpose

    pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Worm and Yeast

    Mutation

    Promoter

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRR-ERalpha-5Z

    Plasmid

    #23061

    Depositor

    Charles Miller

    Article

    Miller et al Plasmid. 2009 Dec 3. ():.

    Insert

    ERalpha (ESR1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

37. pNH605-PACE-YFP

    Plasmid

    #172002

    Purpose

    Fluorescent reporter for Rpn4 activity

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    4xPACE-YFP
    

    Use

    Vector for genomic integration into the yeast gen…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1 core promotor with 4 tandem repeats of Rpn4 …

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ZJOM44

    Plasmid

    #133653

    Purpose

    Integration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SNF7-mCherry (SNF7 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. BB3nK_ext_AC

    Plasmid

    #108686

    Purpose

    Plasmid for assembly of donor DNA templates consisting of two expression units

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGKB

    Plasmid

    #85077

    Purpose

    expression in Pichia pastoris

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge