8,010 results

1. Designed VEGF-binding scFv G6des1

   Plasmid

   #110212

   Purpose

   Yeast Surface Display of G6des1

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des1
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCfB4787

   Plasmid

   #106147

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBS-SKII-3XHA-HALO-NAT

   Plasmid

   #188931

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-NAT
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS-SKII-3XHA-fSNAP-Hygromycin

   Plasmid

   #188914

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fSNAP-Hygromycin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDZ276 (pURA MET28 PCP-2x-yeGFP)

   Plasmid

   #35194

   Depositor

   Robert Singer

   Article

   Larson et al Science. 2011 Apr 22;332(6028):475-8.

   Insert

   PCP-2x-yeGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. PhyB-mCherry-Tom7

   Plasmid

   #66571

   Purpose

   Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae

   Depositor

   Orion Weiner

   Article

   Jost et al ACS Synth Biol. 2015 Jun 2.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   Tom7 and mCherry

   Expression

   Yeast

   Mutation

   PhyB portion contains aa's 1-908

   Promoter

   pADH1

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p4A8_S7T_BC

   Plasmid

   #217832

   Purpose

   4A8 variant in barcoded, yeast surface display plasmid

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   4A8 variant Fab
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   VL: S7T

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKmK.P6

   Plasmid

   #125039

   Purpose

   Storage plasmid for K.marxianus promoter; type 2 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   INU1 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKmK.P5

   Plasmid

   #125038

   Purpose

   Storage plasmid for K.marxianus promoter; type 2 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   HSP150 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKmK.P18

    Plasmid

    #125051

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    XYL2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKmK.P14

    Plasmid

    #125047

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    SSA2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKmK.P13

    Plasmid

    #125046

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    HSP104 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKmK.P17

    Plasmid

    #125050

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    XYL1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKmK.P9

    Plasmid

    #125042

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    ALD4 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG415GPD-EGFP-ccdB

    Plasmid

    #14314

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSN068

    Plasmid

    #101749

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWL89A mPing

    Plasmid

    #145787

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Susan Wessler, Charles Nathan Hancock

    Article

    Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

    Insert

    mPing
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pJZC522

    Plasmid

    #62279

    Purpose

    MCP-VP64 fusion

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    MCP-VP64
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Promoter

    pAdh

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST-DHFR F[1,2]-N (TRP1)

    Plasmid

    #177797

    Purpose

    Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[1,2]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV0761

    Plasmid

    #133494

    Purpose

    Expression of nucleo-cytoplasmic mTagBFP2 in P-cells

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFA6a-his3-P81nmt1-3FLAG

    Plasmid

    #19341

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Aug 26. ():.

    Insert

    his3 (his3 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKmK.T4

    Plasmid

    #125056

    Purpose

    Storage plasmid for K.marxianus terminator; type 4 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    PDC1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pATP416-­pluxO5-­pphlO6-­crtYBI

    Plasmid

    #165977

    Purpose

    Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pluxO5), Synthetic promoter (…

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA6a-link-yEmCFP-Hygro

    Plasmid

    #44644

    Depositor

    Kurt Thorn

    Insert

    yEmCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A206R

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pESC-ARG (V5 and HA)

    Plasmid

    #222493

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and ARG1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pESC-MET

    Plasmid

    #222482

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pESC-LYS

    Plasmid

    #222483

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pESC-TYR

    Plasmid

    #222484

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and TYR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pESC-MET (V5 and HA)

    Plasmid

    #222490

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and MET15 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pESC-LYS (V5 and HA)

    Plasmid

    #222491

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and LYS2 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pESC-TYR (V5 and HA)

    Plasmid

    #222492

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and TYR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pESC-THR (V5 and HA)

    Plasmid

    #222494

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and THR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pESC-THR

    Plasmid

    #222479

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and THR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pESC-ADE

    Plasmid

    #222480

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and ADE2 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pESC-ARG

    Plasmid

    #222481

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and ARG1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ymTq2-yeVenus pDRF1-GW

    Plasmid

    #168076

    Purpose

    FRET fusion between ymTq2 and yeVenus for use in yeast

    Depositor

    Bas Teusink

    Insert

    ymTq2-yeVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. gRNA-leu-HYB

    Plasmid

    #64332

    Purpose

    leu2 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of leu2 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pYZ959

    Plasmid

    #192603

    Purpose

    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    ScTEF1

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 3410 pBM272 Bcl-XL

    Plasmid

    #8772

    Depositor

    Stanley Korsmeyer

    Article

    Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

    Insert

    Bcl-XL (Bcl2l1 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

40. pAG426GAL-EGFP

    Plasmid

    #166143

    Purpose

    EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge