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Yeast Expression

8,010 results

Showing: 61 - 90 of 8010 results

  2. pGADT7-αC

    Plasmid
    #197650
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-2 αC
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Ser677T substitution
    Promoter
    ADH1
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197650-plasmid-map-sequence-id-388876

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  8. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60621-plasmid-map-sequence-id-208224

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  10. ZJOM90

    Plasmid
    #133647
    Purpose
    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pTPI1-SS-mCherry-HDEL
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133647-plasmid-map-sequence-id-292102

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  12. pLH_Scr15

    Plasmid
    #100537
    Purpose
    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    N-terminal version of PhyB (AA 1-621)/ Mutation o…
    Promoter
    FBA1 and TDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100537-plasmid-map-sequence-id-194456

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  15. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

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  16. pBHM2403

    Plasmid
    #171687
    Purpose
    pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    CnoCAS9
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171687-plasmid-map-sequence-id-340174

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  17. pFA6a-link-yemiRFP670-Kan

    Plasmid
    #224088
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with Kan selection
    Depositor
    Bas Teusink
    Insert
    yemiRFP670
    Use
    Tags
    yemiRFP670
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224088-plasmid-map-sequence-id-452414

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  18. ZJOM143

    Plasmid
    #133667
    Purpose
    Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133667-plasmid-map-sequence-id-265896

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  19. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    91248-plasmid-map-sequence-id-192174

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  20. ZJOM68

    Plasmid
    #133657
    Purpose
    Integration of TGL3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-TGL3-mCherry (TGL3 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133657-plasmid-map-sequence-id-268966

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  21. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100539-plasmid-map-sequence-id-194476

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  22. ZJOM119

    Plasmid
    #133661
    Purpose
    Integration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    133661-plasmid-map-sequence-id-265756

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  26. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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Showing: 61 - 90 of 8010 results