8,010 results

1. pDM291-tetR'-tup11Δ70

   Plasmid

   #41028

   Depositor

   Michael Nick Boddy

   Article

   Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

   Insert

   tetR'-tup11Δ70
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   tup11 starts at aa70

   Promoter

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGWKS7

   Plasmid

   #139414

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSC5GGv2

   Plasmid

   #188604

   Purpose

   pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Osa-pCAMBIA-1301-STTM319

   Plasmid

   #84201

   Purpose

   Disturb the expression of miR319 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR319
   

   Use

   Tags

   Expression

   Bacterial, Plant, and Yeast…

   Mutation

   Promoter

   2x35S

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046663

   Plasmid

   #153857

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pESC-URA-GFP-Ubc9wt

   Plasmid

   #20368

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

7. EZ-L545

   Plasmid

   #173024

   Purpose

   Encodes OptoEXP2 circuit

   Depositor

   Jose Avalos

   Article

   Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

   Insert

   VP16-EL222
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKmK.P15

   Plasmid

   #125048

   Purpose

   Storage plasmid for K.marxianus promoter; type 2 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   TDH3 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPS2040

   Plasmid

   #1372

   Depositor

   Pamela Silver

   Article

   Brodsky et al RNA 2000 Dec;6(12):1737-49.

   Insert

   PGK1-U1A-ASH1 3' UTR (PGK1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pCfB2193

    Plasmid

    #67542

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPS2035

    Plasmid

    #1368

    Depositor

    Pamela Silver

    Article

    Brodsky et al RNA 2000 Dec;6(12):1737-49.

    Insert

    U1A (1-94) (RNU1-4 Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    U1A (1-94)

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pGBT9-Hook3

    Plasmid

    #198525

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook3 (HOOK3 Human)
    

    Use

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB4281

    Plasmid

    #98700

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDB4283

    Plasmid

    #98702

    Purpose

    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    gRNA PCR template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS416 GAL1pro-YFP

    Plasmid

    #224875

    Purpose

    Low copy plasmid with GAL1-driven YFP for C-terminal tagging (no start codon)

    Depositor

    Paul Herman

    Type

    Empty backbone

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ZJOM86

    Plasmid

    #133634

    Purpose

    Integration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ANP1-GFP (ANP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZJOM157

    Plasmid

    #133640

    Purpose

    Integration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-GFP-Pep12 (PEP12 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPT24

    Plasmid

    #184419

    Purpose

    Transformation of mitochondrial DNA in yeast

    Depositor

    Martin Ott

    Article

    Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJO629

    Plasmid

    #104342

    Purpose

    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

    Insert

    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPMVAu5 (R)

    Plasmid

    #98296

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LHP306 - Ub - no Lys

    Plasmid

    #32150

    Depositor

    Linda Hicke

    Article

    Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pCfB6574

    Plasmid

    #106132

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJZC638

    Plasmid

    #62283

    Purpose

    Expresses MCP-VP64 and dCas9 in Yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    MCP-VP64
    dCas9
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Nuclease activity has been deactivated by mutatio…

    Promoter

    pAdh and pGal10

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHES836

    Plasmid

    #89195

    Purpose

    Expresses mKate2 under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRS426-GFP-Osh2-PH

    Plasmid

    #71373

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh2-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa 277-398

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

26. 7851 pUG72-HSC82in

    Plasmid

    #10894

    Depositor

    Susan Lindquist

    Article

    Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

    Insert

    HSC82 (HSC82 Budding Yeast)
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACT2.2

    Plasmid

    #11343

    Depositor

    Guy Caldwell

    Type

    Empty backbone

    Use

    Tags

    Gal4 Activation Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

28. p413TEF-PKM2

    Plasmid

    #34608

    Depositor

    Markus Ralser

    Article

    Bluemlein et al Oncotarget. 2011 May;2(5):393-400.

    Insert

    PKM2 (PKM Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKmK.T5

    Plasmid

    #125057

    Purpose

    Storage plasmid for K.marxianus terminator; type 4 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    PGK1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-link-yoTagRFP-T-SpHis5

    Plasmid

    #44842

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p413TEF1-ATR1(pYL573)

    Plasmid

    #178288

    Purpose

    Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    ATR1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMTU-DO-G418

    Plasmid

    #160007

    Purpose

    Low-copy expression vector for K. marxianus, G418 selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKmK.P16

    Plasmid

    #125049

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    FBA1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKT0210

    Plasmid

    #8724

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECFP A206R
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPS2042

    Plasmid

    #1373

    Depositor

    Pamela Silver

    Article

    Brodsky et al RNA 2000 Dec;6(12):1737-49.

    Insert

    U1A (1-94) (RNU1-4 Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    U1A (1-94)

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

37. pYC56

    Plasmid

    #63910

    Purpose

    Recipient integrative vector to construct mCherry tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGBW-m4046647

    Plasmid

    #153940

    Purpose

    Yeast expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. WT (DVD)

    Plasmid

    #184316

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pIM573

    Plasmid

    #74637

    Purpose

    alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    ymTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge