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Yeast Expression

8,030 results

Showing: 1021 - 1050 of 8030 results

  1. pDS275

    Plasmid
    #34972
    Purpose
    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).
    Depositor
    Michael Glotzer
    Article
    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm
    Insert
    Mid2(SS/TM)-GFP-LOVpep
    Use
    Tags
    Expression
    Yeast
    Mutation
    T406A, T407A, I532A
    Promoter
    Available since
    March 23, 2012
    Availability
    Academic Institutions and Nonprofits only
    34972-plasmid-map-sequence-id-42929

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  6. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  8. pNB2289

    Plasmid
    #113396
    Purpose
    Galactose-inducible red fluorescent protein (RFP) codon optimized for S. cerevisiae
    Depositor
    Suzanne Sandmeyer
    Insert
    Red fluorescent protein, codon optimized for S. cerevisiae
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113396-plasmid-map-sequence-id-218741

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  9. ZJOM153

    Plasmid
    #133642
    Purpose
    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    Vph1-2GFP (VPH1 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133642-plasmid-map-sequence-id-268960

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  11. ZJOM20

    Plasmid
    #133644
    Purpose
    Integration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-PEX1-2GFP (PEX1 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133644-plasmid-map-sequence-id-267523

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  23. ymTq2-yeVenus pDRF1-GW

    Plasmid
    #168076
    Purpose
    FRET fusion between ymTq2 and yeVenus for use in yeast
    Depositor
    Bas Teusink
    Insert
    ymTq2-yeVenus
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168076-plasmid-map-sequence-id-332142

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  24. pJH2971

    Plasmid
    #100955
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
    Depositor
    James Haber
    Article
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100955-plasmid-map-sequence-id-193248

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  25. pYeDP60-hATP13A2-WT

    Plasmid
    #171377
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Peter Vangheluwe
    Article
    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:
    Insert
    human ATP13A2 variant 2 wild-type
    Use
    Tags
    BAD tag
    Expression
    Yeast
    Mutation
    Promoter
    URA3
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171377-plasmid-map-sequence-id-342929

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  26. pCfB2225

    Plasmid
    #67553
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67553-plasmid-map-sequence-id-116384

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Showing: 1021 - 1050 of 8030 results