8,010 results

1. pXNgate22-1HA

   Plasmid

   #105077

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p406 – TDH3-Elo3-GFP11-Ura

   Plasmid

   #177734

   Purpose

   ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   Elo3 (ELO3 Budding Yeast)
   

   Use

   Tags

   GFP11 and HDEL

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIp TRP1-HphMx

   Plasmid

   #122922

   Purpose

   TRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMJ240: pRS426-His-MBP-MmeFz2_Native

   Plasmid

   #205255

   Purpose

   S. cerevisiae expression vector for MmeFz2 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   MmeFz2
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVG1

   Plasmid

   #111444

   Purpose

   Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgScADE2/stop-codon repair
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVW296

   Plasmid

   #139496

   Purpose

   Expression vector for the PP7-mCherry protein in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pADH1 PP7∆FG-mCherry tCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ∆FG

   Promoter

   pADH1

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp128-P30-His-POL30

   Plasmid

   #99542

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   POL30

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKmK.I3R

   Plasmid

   #125065

   Purpose

   Storage plasmid for K.marxianus right homology arm

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   Right homology arm for targeting integration to SWF1/ARO1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKmK.I1R

   Plasmid

   #125063

   Purpose

   Storage plasmid for K.marxianus right homology arm

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   Right homology arm for targeting integration to LAC4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYX113-mtGFP

    Plasmid

    #45049

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pKmK.I1L

    Plasmid

    #125030

    Purpose

    Storage plasmid for K.marxianus left homology arm; type 1 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Left homology arm for targeting integration to LAC4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKmK.I3L

    Plasmid

    #125032

    Purpose

    Storage plasmid for K.marxianus left homology arm; type 1 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Left homology arm for targeting integration to SWF1/ARO1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Htb2-GFP

    Plasmid

    #117669

    Purpose

    Colorize the nuclei

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    Htb2-GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains the gene encoding the Histone Htb2 from …

    Promoter

    pGAP

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pI1-MTU-DO-URA

    Plasmid

    #160009

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG306-GPD-VMA1 chr I

    Plasmid

    #41896

    Purpose

    Yeast expression of VMA1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VMA1 (VMA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains silent mutation at 342A-G and L87S mutat…

    Promoter

    GPD

    Available since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YIp TRP1-KanMx

    Plasmid

    #122923

    Purpose

    TRP1-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

    Plasmid

    #194519

    Purpose

    Dual fluorescence reporter for studying protein degradation

    Depositor

    Tommer Ravid

    Article

    Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

    Insert

    TIF4631 leader sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG416GAL10-HRV-B14_3C

    Plasmid

    #203466

    Purpose

    Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    HRV-B14 3C protease (HRVBgp1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-ymTurquoise2-CaURA3

    Plasmid

    #118458

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG306GAL-ccdB

    Plasmid

    #14139

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHCMM4

    Plasmid

    #170359

    Purpose

    Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter

    Depositor

    Hugo Cesar Medina-Munoz, Marvin Wickens

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PCYC1_SEC61_mCherry_LEU2_TCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PCYC1

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC-TALO8

    Plasmid

    #83409

    Purpose

    TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    TRP-ARS-8xLacO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Please see depositor comments below

    Promoter

    TRP1

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPS2044

    Plasmid

    #1375

    Depositor

    Pamela Silver

    Article

    Brodsky et al RNA 2000 Dec;6(12):1737-49.

    Insert

    RPL25-U1A-PGK1 3' UTR (RPL25 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

24. pDS244

    Plasmid

    #34982

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Ste11 (STE11 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YIp TRP1-NatMx

    Plasmid

    #122924

    Purpose

    TRP1-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKmK.C2

    Plasmid

    #125060

    Purpose

    Storage plasmid for K.marxianus centromere

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    KmARS/CEN5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PhyB-mCherry-CAAX

    Plasmid

    #51567

    Purpose

    Constitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    CAAX and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDRF1-GW AKAR3-EV

    Plasmid

    #182533

    Purpose

    Expresses the PKA FRET sensor AKAR3-EV in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

    Insert

    AKAR3-EV
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PMA1

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMEL16

    Plasmid

    #107922

    Purpose

    HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Plasmid

    #58440

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPICZc-HsACTB*-thymosinB-8His

    Plasmid

    #111146

    Purpose

    Expresses human beta-actin fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    ACTB (ACTB Human)
    

    Use

    Pichia pastoris integration

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJZC603

    Plasmid

    #62317

    Purpose

    sgRNA with 2x PP7 for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x PP7 RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pWL89A mmPing20

    Plasmid

    #145788

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T162C, T258A, T287C, T300A, T304A, T310A, G372A

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAG426GPD-Cerulean-ccdB

    Plasmid

    #14420

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pICXNHG3

    Plasmid

    #49021

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Use

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

    Plasmid

    #179074

    Purpose

    Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TEF2p-Scarlet(rfp)-Link-2xPH
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMJA180

    Plasmid

    #128538

    Purpose

    Expresses E coli Bir A protein

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    E coli BirA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVG47

    Plasmid

    #164705

    Purpose

    yEVenus under the control of tetOpr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    tetOpr - yEVenus - CLN2 PEST - ADH1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPS1622

    Plasmid

    #8854

    Depositor

    Pamela Silver

    Article

    Prinz et al J Cell Biol. 2000 Aug 7. 150(3):461-74.

    Insert

    Sec63-sGFP (SEC63 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    sGFP=S65T V163A mutant of GFP

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only