8,173 results
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Plasmid#25445DepositorInsertSec13-Green Fluorescent Protein
ExpressionYeastAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
EC2_7_dCas9_HC_sgRNA
Plasmid#163711PurposeHigh copy plasmid with dCas9 and sgRNA expression cassettesDepositorInsertdCas9
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIM700
Plasmid#74639Purposealias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection markerDepositorInsertyTagRFP-T
ExpressionYeastPromoternoneAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mScarlet1.4-T2A-mTurquoise2
Plasmid#219849Purposeequimolar expression of mScarlet1.4 and mTurquoise2 in budding yeastDepositorInsertmScarlet1.4-T2A-mTurquoise2
ExpressionYeastAvailable SinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pML107-kanR468
Plasmid#162041PurposeExpresses Cas9 and contains kanR468 guide which targets the kanR geneDepositorInsertkanR468 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p426 25Q GAL
Plasmid#1187DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS
Plasmid#181711PurposeGalactose inducible expression of FUSDepositorInsertFUS (FUS Human)
ExpressionYeastAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mStayGold-T2A-mCherry
Plasmid#219834Purposeequimolar expression of mStaygold and mCherry in budding yeastDepositorInsertmStayGold-T2A-mCherry
ExpressionYeastAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
Plasmid#140153PurposeExpresses a regulatable vacuolar cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
ExpressionYeastPromoterTPI1Available SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP(iL)-Grx1(human)
Plasmid#65002PurposeYeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1DepositorAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJL-mGold
Plasmid#157997PurposeExpression of mGold in yeast cellsDepositorInsertmGold
ExpressionYeastMutationmGold is mVenus with L46F;T63S mutationsPromoterpTDH(GAP promoter)Available SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS5
Plasmid#139412PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.DepositorInsertCitrine
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046233
Plasmid#149032PurposeYeast Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p426 72Q GPD
Plasmid#1183DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-GW
Plasmid#63780Purposeyeast expression vector p414GPD modified such that it is Gateway converted and introduces a 3xFLAG tag at the N-terminus of the insertDepositorTypeEmpty backboneTags3xFLAGExpressionYeastPromoterGPDAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJH1260
Plasmid#36913DepositorInsertsScURA3
ScURA3
ExpressionBacterial, Mammalian, and Y…Available SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWS3868
Plasmid#185745PurposeSub-array backbone 4/4DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-HphMx
Plasmid#122916PurposeADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP2_leu2d_NRT1.1
Plasmid#58332PurposeExpresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged proteinDepositorAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0478
Plasmid#133498PurposeExpression of sfGFP targeted to the nucleus with two NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LPH2782 - Ub-K48R
Plasmid#32161DepositorExpressionYeastMutationK48RPromoterCUP1Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
TagsGFP and hphExpressionBacterial and YeastPromoterSpun Hsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYC34
Plasmid#63915PurposeRecipient overexpressing vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneTagsmCherryExpressionYeastPromoterTEF1 (Saccharomyces cerevisiae)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS1885
Plasmid#177341PurposeExpresses Aequorin in Candida albicans using the dominant marker SAT1DepositorInsertAequorin
ExpressionYeastPromoterACT1Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINER5
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
ExpressionYeastMutationNoneAvailable SinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEV-G4-Ph
Plasmid#46820PurposeTEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and HXT7Available SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pABY-c25
Plasmid#201769PurposeExpresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeastDepositorInsertNbALFA
Tags40aa Linker, mNeonGreenExpressionYeastPromoterCYC1Available SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB4666
Plasmid#106144PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046483
Plasmid#153868PurposeYeast expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTCW007
Plasmid#83525Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha1
ExpressionYeastPromoterpFUS1J2Available SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPTK005-3a-αMF
Plasmid#84964PurposeType 3a, α-mating factor - secretion signalDepositorInsertα-mating factor secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 Cyterm-ytd8oxStayGold
Plasmid#219853Purposeexpression of Cyterm-ytd8oxStayGold in budding yeastDepositorInsertCyterm-ytd8oxStayGold
ExpressionYeastAvailable SinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-link-yomEos2-SpHis5
Plasmid#44849PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with SpHis5 selectionDepositorInsertyomEos2
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pREF-GFP kan
Plasmid#122061Purposereporter plasmid for termination and terminator override to express GFP. Marked with URA3DepositorInsertsintergenic terminator from yeast IMD2
green fluorescent protein
ExpressionYeastPromoterGAL1 and GAL1 comes in pYES2Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
EZ-L439
Plasmid#169146PurposeEncodes OptoINVRT7 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-LEU2
Plasmid#122905PurposeADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK020
Plasmid#171292PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPalcA syn_NoCrea (ANIA_08979) promoter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGE2.0-i
Plasmid#194639PurposeEmpty pAGE2.0 plasmid with pBAD inducible promoterDepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-HIS
Plasmid#160142PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
M3938 trp1::ADE2 Disruptor Converter
Plasmid#51673PurposeYeast marker swap plasmid for converting TRP1 to ADE2DepositorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-4420-mCherry
Plasmid#218828PurposeYeast surface display of an anti-fluorescene scFv and intracellular expression of mCherryDepositorInsert4-4-20 scFv
ExpressionYeastPromoterGAL1-10Available SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LD209
Plasmid#69182PurposeIntegration of 2GFP tag at Atg9 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg9-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYX223-mtGFP
Plasmid#45051DepositorInsertmtGFP
ExpressionYeastPromoterGALAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJZC518
Plasmid#62277PurposeYeast dCas9 expression plasmidDepositorInsertdCas9
ExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104(WT)
Plasmid#1146DepositorInsertHSP104 (HSP104 Budding Yeast)
ExpressionYeastAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
p42Hyg 1-F GW
Plasmid#58551PurposeMulticopy vector with Hygromycin resistance marker for single Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
Plasmid#58441PurposeCassette to replace yeast promoters with an inducible promoter containing 3 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
ExpressionYeastPromoterPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EMCV_3C
Plasmid#201941PurposeExpresses EMCV 3C protease from a GAL promoter with a URA3 markerDepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPTK020-7-attB
Plasmid#84989PurposeType 7, BxbI recognition siteDepositorInsertBxbI recognition site
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1711 t to a)PromoterNonAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only