7,933 results

1. pRS303 Gal TDP-43 YFP

   Plasmid

   #27468

   Depositor

   Aaron Gitler

   Article

   Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   D23A mutation in TDP-43 sequence

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pKar2-msGFP-HDEL

   Plasmid

   #117668

   Purpose

   Localisation of Endoplasmic Reticulum

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pKar2-msGFP-HDEL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Contains the Kar2 promoter and signal sequence fr…

   Promoter

   pKar2

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p415TEF cyto roGFP(iL)-Grx1(human)

   Plasmid

   #65002

   Purpose

   Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Tags

   roGFP(iL)

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pH

   Plasmid

   #64178

   Purpose

   harbours marker gene HIS3

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHRW32

   Plasmid

   #130972

   Purpose

   HyPr plasmid with ble marker

   Depositor

   Chris Todd Hittinger

   Article

   Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

   Insert

   empty backbone
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG306GPD-ccdB

   Plasmid

   #14140

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRG710_lexO-HO_LexA-TF_HIS3MX

   Plasmid

   #154819

   Purpose

   Inducible expression of HO endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   HO endonuclease (HO Budding Yeast)
   LexA-ER-B112
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179051

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-RPC19-Link-Scarlet(rfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNXgate32-3HA

   Plasmid

   #105076

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   3xHA and NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pABY-c15

    Plasmid

    #201768

    Purpose

    Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    NbALFA
    

    Use

    Tags

    40aa Linker, mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p426*-LbSgH

    Plasmid

    #136992

    Purpose

    Helper plasmid for LbCpf1 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJY3919

    Plasmid

    #113332

    Purpose

    Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHES941

    Plasmid

    #87942

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. M3929 his3::KanMX3 Disruptor Converter

    Plasmid

    #51679

    Purpose

    Yeast marker swap plasmid for converting HIS3 to KanMX3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    KanMX3
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1x_a1gRNAOp_pCYC1m_yeBFP2

    Plasmid

    #64390

    Purpose

    encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    yeast enhanced BFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG426GPD-EGFP-ccdB

    Plasmid

    #14324

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-mMaple3-kanMX6

    Plasmid

    #105165

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

    Insert

    mMaple3-kanMX6
    

    Use

    Tags

    GlySerGly-mMaple3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG416GAL-ccdB-HA

    Plasmid

    #14243

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEM09

    Plasmid

    #135479

    Purpose

    Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr
    

    Use

    Tags

    GFP and hph

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Spun Hsp70pr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pI2-MTU-DO-URA

    Plasmid

    #160015

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pI1-MTU-DO-LEU

    Plasmid

    #160013

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAWH

    Plasmid

    #133894

    Purpose

    Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBKWH

    Plasmid

    #133898

    Purpose

    Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pREF-GFP kan

    Plasmid

    #122061

    Purpose

    reporter plasmid for termination and terminator override to express GFP. Marked with URA3

    Depositor

    Daniel Reines

    Article

    Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

    Inserts

    intergenic terminator from yeast IMD2
    green fluorescent protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL1 comes in pYES2

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pE3c

    Plasmid

    #17463

    Purpose

    modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    6xMyc

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFA6-mTFP1-KanMX6

    Plasmid

    #69851

    Purpose

    C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mTFP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pICXNH3

    Plasmid

    #49020

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pL-RPB1

    Plasmid

    #10981

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type RPB1 gene

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pESC-URA-Ub-G76A-Rnq1-GFP

    Plasmid

    #21052

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP and ubiquitin

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pCDB24

    Plasmid

    #91959

    Purpose

    empty expression vector for 10His-SUMO tag protein purification

    Depositor

    Christopher Bahl

    Type

    Empty backbone

    Use

    Tags

    10-His and Smt3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pWS481

    Plasmid

    #123029

    Purpose

    LexA UAS

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (6x) [2a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EC2_7_dCas9_HC_sgRNA

    Plasmid

    #163711

    Purpose

    High copy plasmid with dCas9 and sgRNA expression cassettes

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFA6a-link-yomKeima-CaURA3

    Plasmid

    #44885

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKeima with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKeima
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRS426-GFP-PLCdelta-PH

    Plasmid

    #71375

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    PLCdelta-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain

    Promoter

    Available since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pRS416 Gal M337V YFP

    Plasmid

    #27449

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pHRW40

    Plasmid

    #130973

    Purpose

    HyPr plasmid with nptII marker

    Depositor

    Chris Todd Hittinger

    Article

    Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

    Insert

    empty backbone
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAV0327

    Plasmid

    #133488

    Purpose

    Expression of nucleo-cytoplasmic sfGFP

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL

    Plasmid

    #140153

    Purpose

    Expresses a regulatable vacuolar cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

    Insert

    pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTAJAK-71 (pESC-NatMXsyn-USER)

    Plasmid

    #78232

    Purpose

    Empty vector, for the insertion of gRNA expression cassettes into the vector.

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAV0753

    Plasmid

    #133487

    Purpose

    Expression in fission yeast from promoter pnmt81 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge