8,265 results
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Plasmid#84964PurposeType 3a, α-mating factor - secretion signalDepositorInsertα-mating factor secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWS926
Plasmid#123052PurposeLexA bacterial repressorDepositorInsertLexA-NLS [3a]
ExpressionYeastAvailable SinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-G418
Plasmid#160018PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2792
Plasmid#63653PurposepCfB2792 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_GLuc-ns
Plasmid#226719PurposePlasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)DepositorInsertGaussia Luciferase
TagsHA tagExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP95
Plasmid#83550PurposeUsed to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.DepositorInsertPDA1 promoter-yEGFP
ExpressionYeastPromoterPDA1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAct::Raf
Plasmid#11341DepositorAvailable SinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-SpHis5
Plasmid#44849PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with SpHis5 selectionDepositorInsertyomEos2
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
Plasmid#58441PurposeCassette to replace yeast promoters with an inducible promoter containing 3 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
ExpressionYeastPromoterPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL-mGold
Plasmid#157997PurposeExpression of mGold in yeast cellsDepositorInsertmGold
ExpressionYeastMutationmGold is mVenus with L46F;T63S mutationsPromoterpTDH(GAP promoter)Available SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH
Plasmid#64178Purposeharbours marker gene HIS3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM700
Plasmid#74639Purposealias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection markerDepositorInsertyTagRFP-T
ExpressionYeastPromoternoneAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0327
Plasmid#133488PurposeExpression of nucleo-cytoplasmic sfGFPDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KN
Plasmid#63209PurposepCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB3rN_14*
Plasmid#98550Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAL-flp(H305L)-NAT
Plasmid#160380Purposeoverexpression of Flp recombinase step-arrest mutant in yeastDepositorInsertFLP1
ExpressionYeastMutationH305LPromoterGALAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC2_7_dCas9_HC_sgRNA
Plasmid#163711PurposeHigh copy plasmid with dCas9 and sgRNA expression cassettesDepositorInsertdCas9
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG25QDProCFPp303
Plasmid#15584DepositorInserthtt 25Q Delta Pro (HTT Human)
TagsCFP and FLAGExpressionYeastMutationProline-rich region removedAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTCW007
Plasmid#83525Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha1
ExpressionYeastPromoterpFUS1J2Available SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPS2042
Plasmid#1373DepositorAvailable SinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAV0478
Plasmid#133498PurposeExpression of sfGFP targeted to the nucleus with two NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-Cerulean-ccdB
Plasmid#14420DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
Plasmid#140153PurposeExpresses a regulatable vacuolar cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
ExpressionYeastPromoterTPI1Available SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRS415-Gal1pr-Mag1-sctetR
Plasmid#44752DepositorInsertMAG1-sctetR
ExpressionYeastPromoterGal1prAvailable SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPM48
Plasmid#22854DepositorInserteroGFP & UPR-RFP
ExpressionYeastAvailable SinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDS2149
Plasmid#184275PurposeTet-on genome insertion systemDepositorInsertCatrTA
ExpressionYeastPromoterTDH3Available SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ytdTomato-CaURA3
Plasmid#118461PurposeYeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.DepositorInsertytdTomato
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-K2-L2
Plasmid#80684PurposeGateway Entry vector containing a temperature-sensitive mouse DHFRDepositorUseSynthetic BiologyTags3xHAExpressionBacterial, Insect, Mammalia…Mutationmouse DHFR with N-terminal UbiquitinAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB4666
Plasmid#106144PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPS1739
Plasmid#8922DepositorAvailable SinceAug. 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP(iL)-Grx1(human)
Plasmid#65002PurposeYeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1DepositorAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG424GPD-EGFP-ccdB
Plasmid#14320DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104(WT)
Plasmid#1146DepositorInsertHSP104 (HSP104 Budding Yeast)
ExpressionYeastAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pYX223-mtGFP
Plasmid#45051DepositorInsertmtGFP
ExpressionYeastPromoterGALAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal G294A YFP
Plasmid#27448DepositorInsertTDP-43 (TARDBP Human)
TagsYFPExpressionYeastMutationGlycine 294 mutated to Alanine (G294A)Available SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 yeBFP2-T2A-mCherry
Plasmid#219851Purposeequimolar expression of yeBFP2 and mCherry in budding yeastDepositorInsertyeBFP2-T2A-mCherry
ExpressionYeastAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yomTagBFP2-T2A-mCherry
Plasmid#205493PurposeExpression of yomTagBFP2-T2A-mCherry in yeastDepositorInsertyomTagBFP2-T2A-mCherry
ExpressionYeastAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 Empty
Plasmid#169710PurposeYeast two-hybrid vector using a copper inducible CUP1 promoterDepositorArticleTypeEmpty backboneTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yomCherry-T2A-mTurquoise2
Plasmid#118447PurposeProduces equimolar expression of yeast codon-optimized mCherry and mTq2DepositorInsertyomCherry-T2A-mTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUDE1093
Plasmid#204227PurposeEpisomal yeast plasmid expressing the Ercas12aDepositorInsertEubacterium rectale cas12a (Ercas12a)
ExpressionYeastMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1Available SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJA065
Plasmid#128517PurposeExpresses Ber e 1 in PichiaDepositorInsertBer e 1 a allergenic 2S albumin from Brazil nut
ExpressionYeastPromoterAOX1Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p42Hyg 1-F GW
Plasmid#58551PurposeMulticopy vector with Hygromycin resistance marker for single Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
TagsGFP and hphExpressionBacterial and YeastPromoterSpun Hsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLSPR24
Plasmid#217309PurposePromoterless (SwaI Cutsite) mRuby3:amdS fusion reporterDepositorInsertAcetamidase
TagsmRuby3ExpressionYeastAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR25
Plasmid#217310PurposePromoterless (SwaI Cutsite) mNeonGreen:amdS fusion reporterDepositorInsertAcetamidase
TagsmNeonGreenExpressionYeastAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR30
Plasmid#217315PurposeGAL7(p)-mRuby3:amdS fusion reporterDepositorInsertAcetamidase
TagsmRuby3ExpressionYeastPromoterGAL7Available SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR31
Plasmid#217316PurposeTEF1(p)-mRuby3:amdS fusion reporterDepositorInsertAcetamidase
TagsmRuby3ExpressionYeastPromoterTEF1Available SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR32
Plasmid#217317PurposeGAL7(p)-mNeonGreen:amdS fusion reporterDepositorInsertAcetamidase
TagsmNeonGreenExpressionYeastPromoterGAL7Available SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR33
Plasmid#217318PurposeTEF1(p)-mNeonGreen:amdS fusion reporterDepositorInsertAcetamidase
TagsmNeonGreenExpressionYeastPromoterTEF1Available SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only