8,057 results

1. pFA6a-GBP-mCherry-kanMX6

   Plasmid

   #89068

   Purpose

   genomic GBP-mCherry epitope C-terminal tagging in S. pombe

   Depositor

   Quanwen Jin

   Article

   Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   GBP-mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG305GPD-ccdB

   Plasmid

   #14138

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9

   Plasmid

   #162606

   Purpose

   Pre-express Cas9 in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGal4BD-CRY2PHR

   Plasmid

   #28244

   Purpose

   Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   cry2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Gal4BD

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG424GAL-ccdB

   Plasmid

   #14151

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYTK-DN5

   Plasmid

   #180286

   Purpose

   NatR multi-gene yeast expression vector (GFP-dropout)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS' - GFP - ConRE' - NatR - CEN6/ARS4
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB8644

   Plasmid

   #126919

   Purpose

   Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtI and HMG1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJS646

   Plasmid

   #183175

   Purpose

   Y2H vector compatible with pJS647 PYR1 insert

   Depositor

   Timothy Whitehead

   Article

   Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWS173

   Plasmid

   #90960

   Purpose

   Cas9 gap repair vector - KanR

   Depositor

   Tom Ellis

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNB769

    Plasmid

    #60138

    Purpose

    Red click beetle luciferase from Promega driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    CBR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMEG_YLCas9_empty

    Plasmid

    #117826

    Purpose

    CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlpTEF_hCas9_YlCyc1TT
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ZJOM40

    Plasmid

    #133650

    Purpose

    Integration of SEC7-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SEC7-DuDre (SEC7 Budding Yeast)
    

    Use

    Tags

    DuDre

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGS_129

    Plasmid

    #160652

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    pZ estradiol inducible promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. UASGAL-Pgal

    Plasmid

    #60338

    Purpose

    Contains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pEK36

    Plasmid

    #210598

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
    

    Use

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB2191

    Plasmid

    #67538

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. GAL FLAG25QDProGFP p306

    Plasmid

    #15831

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. p416 103Q GPD

    Plasmid

    #1180

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #83946

    Purpose

    Cas9-carrying vector with kanMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKL07

    Plasmid

    #104431

    Purpose

    mitochondrial matrix targeted super ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector

    Depositor

    Joel Kralj

    Article

    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

    Insert

    mito-Super Ecliptic pHluorin-mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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