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8,431 results

Showing: 821 - 840 of 8431 results

  1. p2B-56

    Plasmid
    #177925
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Depositor
    Vadim Gladyshev
    Publication
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    MYO2
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177925-plasmid-map-sequence-id-349995

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  2. SZ_EC_02

    Plasmid
    #155341
    Purpose
    Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR
    Depositor
    Jens Nielsen
    Publication
    Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:
    Inserts
    tef1 promoter - xylulose kinase - pex20 terminator
    pyk1 promoter - xylose reductase - pex16 terminator
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
    Use
    Easycloneyali insert vector
    Expression
    Yeast
    Promoter
    gapdh, pyk1, and tef1
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    155341-plasmid-map-sequence-id-316921

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  6. pCAS9i

    Plasmid
    #141249
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Publication
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141249-plasmid-map-sequence-id-308874

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  7. pAID6

    Plasmid
    #136989
    Purpose
    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4
    Depositor
    Huimin Zhao
    Publication
    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:
    Inserts
    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    Expression
    Yeast
    Promoter
    ENO2, TDH3, TEF1, and TPI1
    Available since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    136989-plasmid-map-sequence-id-269844

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  8. ZJOM20

    Plasmid
    #133644
    Purpose
    Integration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.
    Depositor
    Zhiping Xie
    Publication
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-PEX1-2GFP (PEX1 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133644-plasmid-map-sequence-id-267523

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  9. ZJOM160

    Plasmid
    #133670
    Purpose
    Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.
    Depositor
    Zhiping Xie
    Publication
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pTPI1-mTagBFP2-SKL
    Tags
    mTagBFP2
    Expression
    Yeast
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133670-plasmid-map-sequence-id-267532

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  10. ZJOM31

    Plasmid
    #133645
    Purpose
    Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.
    Depositor
    Zhiping Xie
    Publication
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-TGL3-GFP (TGL3 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133645-plasmid-map-sequence-id-265890

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  11. pFA6a-mNeonGreen-HIS3MX6

    Plasmid
    #129100
    Purpose
    For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen
    Depositor
    Wei-Lih Lee
    Insert
    mNeonGreen
    Expression
    Yeast
    Available since
    July 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129100-plasmid-map-sequence-id-252098

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  13. pV1090

    Plasmid
    #111427
    Purpose
    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)
    Depositor
    Gerald Fink
    Publication
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111427-plasmid-map-sequence-id-212460

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  14. pPICZc-ScAct1*-thymosinB-8His

    Plasmid
    #111148
    Purpose
    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
    Depositor
    Mohan Balasubramanian
    Publication
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    Act1 (ACT1 Budding Yeast)
    Use
    Pichia pastoris integration plasmid
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111148-plasmid-map-sequence-id-214055

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  16. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Publication
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101725-plasmid-map-sequence-id-199888

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  20. pAV126

    Plasmid
    #63192
    Purpose
    pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    63192-plasmid-map-sequence-id-104870

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Showing: 821 - 840 of 8431 results