8,016 results
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Plasmid#124277PurposeExpresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationPromoterAOX1Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HPH
Plasmid#160008PurposeLow-copy expression vector for K. marxianus, hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
G31, Cdc20-AID tagging
Plasmid#130269PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombinationDepositorInsertCdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
UseTagsAID(71-114)ExpressionYeastMutationPromoterNative promoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH728-CEN-RLuc/maxCFLuc
Plasmid#38212DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB6578
Plasmid#106130PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-LEU-C4
Plasmid#160341PurposeHigh-copy expression vector for K. marxianus, leucine selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179067PurposeVector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid dropletDepositorInsertRPL18Bp-ERG6-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)
Plasmid#179066PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosomeDepositorInsertRPL18Bp-Scarlet(rfp)-Link-ATG8
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#64177Purposeharbours marker gene LEU2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC-XII5
Plasmid#176161Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT0090
Plasmid#8714DepositorInsertyEVenus
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179060PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomesDepositorInsertRPL18Bp-PIL1-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT11F
Plasmid#165062PurposeIntroducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiaeDepositorInsertT-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416Gal-GFP
Plasmid#38309DepositorInsertEGFP (eGFP )
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C4
Plasmid#125062PurposeStorage plasmid for K.marxianus ARSDepositorInsertKmARS2
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS35
Plasmid#83797PurposeUseful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selectionDepositorInsertmCherry
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
BS-His3
Plasmid#69197Purposecomplementation of yeast auxotrophic marker HIS3DepositorInsertHIS3
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pGK415-luxTA2-4F
Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSWHhc-p53
Plasmid#133903PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorInsertp53 (TP53 Mouse)
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKeima-SpHis5
Plasmid#44847PurposeYeast tagging vector to create gene fusion with yeast optimized mKeima with SpHis5 selectionDepositorInsertyomKeima
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
YIp204-PADH1-AFB2
Plasmid#99531PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorInsertAFB2 (AFB2 Mustard Weed)
UseTagsExpressionYeastMutationPromoterADH1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPMVAugw
Plasmid#98300PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.DepositorInsertTEFM1EfmvaE>TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMZ222
Plasmid#52223PurposeConstitutive expression of humanized Cas9DepositorInsertCas9
UseTagsExpressionYeastMutationsilent mutation in the original CspCI sitePromoteradh1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUDE731
Plasmid#103008PurposeS. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3pDepositorInsertFncpf1
UseTags3HA and NLSExpressionYeastMutationhuman codon optimizedPromoterTEF1Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP657-CaURA3
Plasmid#44882PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP657 with CaUra3 selectionDepositorInsertyoTagRFP657
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-SpHis5
Plasmid#44845PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selectionDepositorInsertyomKO2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUB23-M-beta-gal
Plasmid#8816DepositorInsertbeta-gal
UseTagsUbiquitinExpressionYeastMutationM (methionine) at amino terminus of beta-galPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)
Plasmid#179054PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubulesDepositorInsertRPL18Bp-Scarlet(rfp)-Link-TUB1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG
Plasmid#176400PurposeExpresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.DepositorInsertFRB-RGG-GFP-RGG-RGG
UseTagsFRB and GFPExpressionYeastMutationPromoterGAL1Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1
Plasmid#60385PurposeExpression of yeast alpha tubulin (TUB1) using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC46
Plasmid#63904PurposeRecipient integrative vector to construct FLAG tagged fusionsDepositorTypeEmpty backboneUseTagsFLAGExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ376
Plasmid#74213PurposeCombined sgRNA/Cas9 vector with KanMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCHAP4
Plasmid#206850PurposeA plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-NLS
Plasmid#51571PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsNLS and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
ADH1pro_GCN4_SIR4
Plasmid#138620PurposeTranscription factor tagged with Sir4 used for Calling Cards experiments for binding measurementDepositorInsertGCN4 (GCN4 Budding Yeast)
UseTagsExpressionYeastMutationPromoterADH1Available sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-ccdB-EGFP
Plasmid#14194DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only