8,173 results
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Plasmid#8819DepositorInsertbeta-gal
TagsUbiquitinExpressionYeastMutationK (lysine) at amino terminus of beta-galAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM166
Plasmid#84197PurposeDisturb the expression of miR166 in riceDepositorInsertmiR166
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179076PurposeVector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulumDepositorInsertTDH3p-Scarlet(rfp)-Link-SCS2tmh
ExpressionYeastAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)
Plasmid#179073PurposeVector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membraneDepositorInsertTDH3p-Scarlet(rfp)-Link-2xPH
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK87 pHomL-RPL18Bp-TOM70-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179071PurposeVector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Scarlet(rfp), a marker for the mitochondriaDepositorInsertRPL18Bp-TOM70-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK85 pHomL-RPL18Bp-PEX25-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179069PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Scarlet(rfp), a marker for the peroxisomeDepositorInsertRPL18Bp-PEX25-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK81 pHomL-RPL18Bp-PRC1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179065PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Scarlet(rfp), a marker for the vacuoleDepositorInsertRPL18Bp-PRC1-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK78 pHomL-RPL18Bp-SNF7-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179062PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Scarlet(rfp), a marker for the endosome, class E compartmentDepositorInsertRPL18Bp-SNF7-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK77 pHomL-RPL18Bp-Scarlet(rfp)-Link-VPS21-SSA1t-HomR (AmpR)
Plasmid#179061PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VPS21, a marker for the endosomesDepositorInsertRPL18Bp-Scarlet(rfp)-Link-VPS21
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK75 pHomL-RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)
Plasmid#179059PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-2xPH(PLCd), a marker for the plasma membraneDepositorInsertRPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK74 pHomL-RPL18Bp-Scarlet(rfp)-Link-SFT2-SSA1t-HomR (AmpR)
Plasmid#179058PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SFT2, a marker for the late GolgiDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SFT2
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK73 pHomL-RPL18Bp-Scarlet(rfp)-Link-SED5-SSA1t-HomR (AmpR)
Plasmid#179057PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SED5, a marker for the early GolgiDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SED5
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK72 pHomL-RPL18Bp-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179056PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SCS2tmh, a marker for the endoplasmic reticulumDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SCS2tmh
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179055PurposeVector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeletonDepositorInsertRPL18Bp-ARC35-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179053PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle poleDepositorInsertRPL18Bp-SPC42-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)
Plasmid#178996PurposeSp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046426
Plasmid#149140PurposeYeast Expression plasmid for SARS-CoV-2 ORF7bDepositorInsertSARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046422
Plasmid#145515PurposeYeast Expression plasmid for SARS-CoV-2 ORF6 proteinDepositorInsertSARS-CoV-2 ORF6 protein (ORF6 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJW1515
Plasmid#62385PurposepFA6a Osm1-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
TagsEGFPExpressionYeastPromoterAdh1pAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETCON-IgG1/design_50
Plasmid#30301DepositorInsertIgG Fc Designed Binder IgG1
ExpressionYeastAvailable SinceJune 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG303GPD-EGFP-ccdB
Plasmid#14302DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS16-iGFPx6
Plasmid#105275PurposePlasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.DepositorAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM12
Plasmid#133638PurposeIntegration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBM4607
Plasmid#18795DepositorAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB-TAP
Plasmid#14275DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-PCYC
Plasmid#60335PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-SKII-3XHA-fCLIP-Kan
Plasmid#188923PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-Kan
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNC953
Plasmid#20402DepositorInsertCFP-reporter
ExpressionYeastMutationX=UbiE (see schematic) linker=deltakAvailable SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
LD206
Plasmid#69181PurposeIntegration of 2GFP tag at Atg6 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg6-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2513
Plasmid#67543PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-EYFP-ccdB
Plasmid#14340DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046406
Plasmid#153880PurposeYeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXP821
Plasmid#46063DepositorTypeEmpty backboneExpressionYeastPromoterGAL1Available SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXP518
Plasmid#26846DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_LT84_RPL13A
Plasmid#177712PurposeExpresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
426Gal-FUS-1-501aa-YFP
Plasmid#29601DepositorAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
LD217
Plasmid#69186PurposeIntegration of 2GFP tag at Atg17 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg17-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046463
Plasmid#145462PurposeYeast Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICE TRP1-NatMx
Plasmid#122903PurposeTRP1-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB3046(gRNA XI-5)
Plasmid#73288PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIp211-P30-His-pol30(K164R)-3'
Plasmid#99548PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; URA3 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXP722
Plasmid#46056DepositorTypeEmpty backboneExpressionYeastPromoterGAL1Available SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4L
Plasmid#104507PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceNov. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
RPS1B-GFP (95)
Plasmid#24039DepositorAvailable SinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-TAP
Plasmid#14267DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pR184
Plasmid#47952PurposertTA positive feedback loop with three tet operators in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionExpressionYeastPromoterGal1Available SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
GTH-g
Plasmid#81104PurposeGene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM2-mRUBY2
Plasmid#107793Purposedonor vector for knock-in gene editing at human DNM2 locusDepositorInsertDNM2-mRuby2 (DNM2 Human, Synthetic)
UseYeast-e.coli shuttle vectorExpressionBacterial and YeastAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM1-EGFP
Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Human, Synthetic)
UseYeast-e.coli shuttle vectorExpressionBacterial and YeastAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only