8,265 results
-
Plasmid#129268Purposedual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporterDepositorInsertRnq1
ExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNC820
Plasmid#20383DepositorInsertUAS cassette (FUS1)
ExpressionYeastMutationX=UbiM (see schematic)Available SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
ExpressionBacterial and YeastPromoterpADH1Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSec7-Halox2
Plasmid#115425PurposeExpress Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-Halox2
TagsHaloTagExpressionYeastPromoterEndogenousAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXRhMX
Plasmid#63146PurposeHPH marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
POM42-mCitrine
Plasmid#69854PurposeN-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.DepositorInsertmCitrine minus start and stop codon
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-MYO1
Plasmid#51570PurposeConstitutively express myosin ring localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsMYO1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
p414TEF_Hsp82_CycTER
Plasmid#61711PurposeYeast plasmid with Hsp82DepositorAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBKWH-Lam
Plasmid#133899PurposeNegative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWHDepositorAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook2
Plasmid#198524PurposeExpression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook2 (HOOK2 Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastMutationHis488Gln substitutionPromoterADH1Available SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXP314
Plasmid#26835DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNC951
Plasmid#20400DepositorInsertCFP-reporter
ExpressionYeastMutationX=UbiY (see schematic) linker=deltakAvailable SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
FRP1454
Plasmid#64510PurposeExpresses destabilized Citrine in yeast.DepositorInsertCitrine-ADH1-tail
ExpressionYeastPromoterTDH3Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ACS1
Plasmid#126712Purposeyeast plasmid complementing ACS1 from Komagataella phaffiiDepositorInsertacs1
Tags6xHis,MycExpressionYeastPromoterGAPAvailable SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXP122
Plasmid#26827DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNB777
Plasmid#60153PurposeRed click beetle luciferase (Promega) driven by LEU1 promoter.DepositorInsertCBR
ExpressionYeastPromoterLEU1prAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-ccdB-EGFP
Plasmid#14192DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pH3F
Plasmid#85773PurposeN-ICE plasmid pH3FDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGB-REL
Plasmid#27277DepositorAvailable SinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBTM116-D9
Plasmid#111232Purposeyeast expression vectorDepositorInsertccdB-cassette
ExpressionYeastAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-ccdB
Plasmid#188445PurposeCloning Vector and pDESTDepositorInsertccdB
ExpressionYeastAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCP8
Plasmid#166110PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5DepositorAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKT0211
Plasmid#8734DepositorInsertyECitrine A206R
TagsCodon optimized linkerExpressionYeastMutationyEGFP with S65G, V68L, Q69M, S72A, T203Y, A206RAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER
Plasmid#52686PurposeSame as 41024, with additional linkerDepositorInserttetO7-Pcyc1-3xHA+LINKER
UsePromoter replacementExpressionYeastAvailable SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046672
Plasmid#153918PurposeYeast expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationwtPromoterpGAL1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
LHP556 - UBC4
Plasmid#32440DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG72QDProCFPp303
Plasmid#15586DepositorInserthtt 72Q Delta Pro (HTT Human)
TagsCFP and FLAGExpressionYeastMutationProline-rich region removedAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
TagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet17
Plasmid#107453PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM394
Plasmid#84204PurposeDisturb the expression of miR394 in riceDepositorInsertmiR394
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303 Bt δCOP
Plasmid#73871PurposeBos taurus Arcn1DepositorInsertRet2 Promoter- COPD
ExpressionYeastPromoterRet2 PromoterAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUDP044
Plasmid#101168PurposepUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
7859 pRS305-pUG72
Plasmid#10897DepositorAvailable SinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
-
pAV0756
Plasmid#133508PurposeExpression of 3mCherry tagged CRIB domain to monitor active Cdc42DepositorInsertpHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP1671
Plasmid#64538PurposeInducible MET15 geneDepositorAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPS2167
Plasmid#1365DepositorAvailable SinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAH233
Plasmid#121144PurposeExpression of gRNA with BbsI placeholder for target cloningDepositorInsertgRNA cassette
UseCRISPRExpressionYeastPromoterS.pombe rrk1Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2
Plasmid#60388PurposeExpression of yeast beta tubulin (TUB2) using a GAL promoterDepositorInsertTUB2
ExpressionYeastPromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPD 104QDProGFP p416
Plasmid#15569DepositorInserthtt 104Q Delta Pro (HTT Human)
TagsGFPExpressionYeastMutationProline-rich region removedAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-STV1-iGFP
Plasmid#177111PurposeExpresses Stv1-iGFP in yeast cellsDepositorInsertSTV1 (STV1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2990
Plasmid#63645PurposepCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUB23-K-beta-gal
Plasmid#8819DepositorInsertbeta-gal
TagsUbiquitinExpressionYeastMutationK (lysine) at amino terminus of beta-galAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJT10R
Plasmid#98307PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1-M7A,H102A
Plasmid#159168PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A,H102A
TagsSV40 NLSExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
mito-yHS1-M7A,H102A
Plasmid#159165PurposeMitochondrial labile heme reporterDepositorInsertmito-yHS1-M7A,H102A
TagsCOX4 pre-sequenceExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
GTU-g
Plasmid#81094PurposeGene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
BB3aN_14*
Plasmid#98548Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-3nmt1-mMaple3
Plasmid#105167PurposeYeast gene targetingDepositorInsertkanMX6-3nmt1-mMaple3
Tags3nmt1-mMaple3ExpressionBacterial and YeastPromoter3nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only