7,935 results

1. BiFC4

   Plasmid

   #190049

   Purpose

   BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

   Depositor

   Patrick Van Dijck

   Article

   Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

   Insert

   YemVenus (ATG-aa173-238)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met3

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BiFC2

   Plasmid

   #190047

   Purpose

   BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

   Depositor

   Patrick Van Dijck

   Article

   Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

   Insert

   YemVenus (aa1-172 - TAA)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met3

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BiFC1

   Plasmid

   #190046

   Purpose

   BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

   Depositor

   Patrick Van Dijck

   Article

   Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

   Insert

   YemVenus (aa1-172)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met3

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLK88 - Edit-directing plasmid base

   Plasmid

   #98814

   Purpose

   Edit-directing plasmid base

   Depositor

   Leonid Kruglyak

   Insert

   CAN1.y gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pK3FS-GAL1

   Plasmid

   #85778

   Purpose

   N-ICE plasmid pK3FS-GAL1

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-KanMX-loxP-GAL1p-3FLAG
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p42Neo 3-F GW

   Plasmid

   #58558

   Purpose

   Multicopy with G418 resistance marker for triple Gateway insertion

   Depositor

   Leonid Kruglyak

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221-Sen1

   Plasmid

   #99994

   Purpose

   Shuttle yeast Sen1 into Gateway destination vector

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Sen1 (SEN1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETCON-HtsptLB12

   Plasmid

   #45120

   Depositor

   David Baker

   Article

   Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

   Insert

   HtsptLB12
   

   Use

   Tags

   Aga2p for yeast surface display and c-myc epitope…

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YIplac211-VPS8-iGFPx6

   Plasmid

   #105271

   Purpose

   Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Vps8 portion (VPS8 Budding Yeast)
   

   Use

   Tags

   iGFPx6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG426Gal-GFP

    Plasmid

    #38310

    Depositor

    Susan Lindquist

    Article

    McLellan et al ACS Chem Biol. 2012 Jun 22.

    Insert

    EGFP (eGFP )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pAG416GAL-ccdB-EYFP

    Plasmid

    #14219

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p404GALS

    Plasmid

    #17435

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GALS promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZMU-Dest

    Plasmid

    #105089

    Purpose

    Yeast expression vector for Gateway cloning and SUS bridge assays

    Depositor

    Christopher Grefen

    Article

    Grefen et al Methods Mol Biol. 2014;1062:659-78. doi:

    Type

    Empty backbone

    Use

    Tags

    myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYES2.1-cerMET3

    Plasmid

    #107436

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET3 (MET3 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG306GAL-ccdB-EGFP

    Plasmid

    #14187

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p405GALS

    Plasmid

    #15965

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    p405GALS (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GALS promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYES2.1-cerMET17

    Plasmid

    #107443

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET17 (MET17 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK148-Pnmt41-GBP-mCherry(N)-leu1+

    Plasmid

    #89070

    Purpose

    genomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombe

    Depositor

    Quanwen Jin

    Article

    Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

    Type

    Empty backbone

    Use

    Genomic integration in s. pombe

    Tags

    GBP-mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMZ284

    Plasmid

    #52225

    Purpose

    Expression of sgRNA precursor targeted against ade6+

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSD02

    Plasmid

    #16009

    Depositor

    Fred Cross

    Article

    Di Talia et al Nature. 2007 Aug 23. 448(7156):947-51.

    Insert

    ACT1pr-DsRed.T1.N1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYES2.1-cerMET2

    Plasmid

    #107435

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET2 (MET2 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKT0146

    Plasmid

    #8742

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    tdimer2
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGBW-m4046381

    Plasmid

    #149010

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pYES2.1-cerMET14

    Plasmid

    #107441

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET14 (MET14 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYIAL31

    Plasmid

    #60807

    Purpose

    used to make mutations in yeast POL1

    Depositor

    Youri Pavlov, Thomas Kunkel

    Article

    Pavlov et al Genetics. 2001 Sep;159(1):47-64.

    Insert

    Pol1 (POL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N-terminal part only

    Promoter

    Available since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG413GAL-EGFP-ccdB

    Plasmid

    #14309

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCfB1975 (pUC19-PTEF1-PPGK1)

    Plasmid

    #73296

    Purpose

    Example plasmid containing a bidirectional promoter

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    pTEF1-pPGK1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGBW-m4046692

    Plasmid

    #149195

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. PhyB-mCherry-SIK1

    Plasmid

    #51577

    Purpose

    Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    SIK1 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LD220

    Plasmid

    #69188

    Purpose

    Integration of 2GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg20-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. FC857

    Plasmid

    #27327

    Depositor

    Fred Cross

    Article

    Cross et al Yeast. 2010 Oct 22. ():.

    Insert

    CDH1 (CDH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C-terminal truncation of CDH1 at its BamHI site

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDEST-DHFR F[3]-C (LEU2)

    Plasmid

    #177796

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[3]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDEST-DHFR F[1,2]-C (TRP1)

    Plasmid

    #177795

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[1,2]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. YEplac181-TYS1

    Plasmid

    #8629

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics 2004 Mar;166(3):1215-27.

    Insert

    TYS1 (TYS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Nov. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBES2

    Plasmid

    #83800

    Purpose

    Useful for tagging yeast proteins with N-terminal Venus tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    Venus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFA6a-link-mKO1-CaUra3

    Plasmid

    #44638

    Purpose

    Yeast tagging vector to create gene fusion with mKO1 with CaUra3 selection

    Depositor

    Kurt Thorn

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFA6a-link-yoPSmOrange-SpHis5

    Plasmid

    #44856

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPSmOrange
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSK260

    Plasmid

    #129252

    Purpose

    dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35 NM yTRAP sensor reporting on mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUB23-R-beta-gal

    Plasmid

    #8818

    Depositor

    Alexander Varshavsky

    Article

    Bachmair et al Science 1986 Oct 10;234(4773):179-86.

    Insert

    beta-gal
    

    Use

    Tags

    Ubiquitin

    Expression

    Yeast

    Mutation

    R (arginine) at amino terminus of beta-gal

    Promoter

    Available since

    Dec. 28, 2005

    Availability

    Academic Institutions and Nonprofits only