8,025 results
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Plasmid#32159DepositorInsertPAN1 (PAN1 Budding Yeast)
UseTagsHAExpressionYeastMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCfB6682
Plasmid#106152PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG426Gal-GFP
Plasmid#38310DepositorInsertEGFP (eGFP )
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-msGFP2-STE13
Plasmid#177106PurposeExpresses msGFP2-Ste13 in yeast cellsDepositorInsertSTE13 (STE13 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo*
Plasmid#115427PurposeExpresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locusDepositorInsertpOst1-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-GAL1
Plasmid#85778PurposeN-ICE plasmid pK3FS-GAL1DepositorInsertloxP-KanMX-loxP-GAL1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFTK051
Plasmid#171323PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteBFP fluorescent reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-HIS
Plasmid#160016PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB4796
Plasmid#106131PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCW002
Plasmid#83522Purposepheromone mediated destablised-GFP expression in yeastDepositorInsertpFUS1-yEGFPCLN2PEST
UseTagsExpressionYeastMutationPromoterpFUS1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMS22H
Plasmid#133904PurposeExpression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWHDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMZ284
Plasmid#52225PurposeExpression of sgRNA precursor targeted against ade6+DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZMU-Dest
Plasmid#105089PurposeYeast expression vector for Gateway cloning and SUS bridge assaysDepositorTypeEmpty backboneUseTagsmycExpressionYeastMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-EYFP
Plasmid#14219DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SIK1
Plasmid#51577PurposeConstitutively express nucleolus localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSIK1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLK88 - Edit-directing plasmid base
Plasmid#98814PurposeEdit-directing plasmid baseDepositorInsertCAN1.y gRNA
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKT0146
Plasmid#8742DepositorInserttdimer2
UseTagsCodon optimized linkerExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNC824
Plasmid#20384DepositorInsertUAS cassette (FUS1)
UseTagsExpressionYeastMutationX=UbiY (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS8-iGFPx6
Plasmid#105271PurposePlasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.DepositorInsertVps8 portion (VPS8 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-EGFP-ccdB
Plasmid#14309DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pUB23-R-beta-gal
Plasmid#8818DepositorInsertbeta-gal
UseTagsUbiquitinExpressionYeastMutationR (arginine) at amino terminus of beta-galPromoterAvailable sinceDec. 28, 2005AvailabilityAcademic Institutions and Nonprofits only -
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THI VP64 pGAP T5
Plasmid#161492PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T5 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T1
Plasmid#161488PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T1 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPSmOrange-SpHis5
Plasmid#44856PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selectionDepositorInsertyoPSmOrange
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK148-Pnmt41-GBP-mCherry(N)-leu1+
Plasmid#89070Purposegenomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p404GALS
Plasmid#17435DepositorInsertGALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGALS promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUB23-P-beta-gal
Plasmid#8817DepositorInsertbeta-gal
UseTagsUbiquitinExpressionYeastMutationP (proline) at amino terminus of beta-galPromoterAvailable sinceDec. 28, 2005AvailabilityAcademic Institutions and Nonprofits only -
LD220
Plasmid#69188PurposeIntegration of 2GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg20-2EGFP
UseTags2xEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTAL6-BB
Plasmid#36034DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPTEF1Available sinceJuly 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046381
Plasmid#149010PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046692
Plasmid#149195PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pTH644-CENBEVY
Plasmid#29695DepositorInsertbidirectional promoter cassette from pBEVY-U
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
LHP336 - HA-Ub-K48R
Plasmid#32164DepositorUseTagsHAExpressionYeastMutationK48RPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSD02
Plasmid#16009DepositorInsertACT1pr-DsRed.T1.N1
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-3FLAG
Plasmid#19336DepositorInsert3FLAG
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*
Plasmid#89071Purposegenomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only