7,935 results
-
-
-
pML104-kanR1136
Plasmid#162040PurposeExpresses Cas9 and contains kanR1136 guide which targets the kanR geneDepositorInsertkanR1136 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPS808
Plasmid#8856DepositorTypeEmpty backboneUseTagsGFPExpressionYeastMutationPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
PP363
Plasmid#78984Purpose255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.DepositorInsertsrHGH
IFNa2b
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR208IIM1
Plasmid#45352DepositorInsertUBR1 mutant
UseTagsExpressionYeastMutationP406S, DE317NKPromoterADHAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046457
Plasmid#145424PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXP732
Plasmid#46058DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterCUP1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pGBW-m4046390
Plasmid#148996PurposeYeast Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJZC593
Plasmid#62319PurposesgRNA with MS2-PP7 for yeast cellsDepositorInsertsgRNA + MS2-PP7 RNA binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHUM
Plasmid#64170Purposeharbours marker genes HIS3, URA3 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
JME4393
Plasmid#129657PurposeLYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB813
Plasmid#60161PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.DepositorInsertyEVenus-PEST
UseTagsPEST degronExpressionYeastMutationPromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLK89 - Edit-directing plasmid cloning insert
Plasmid#98846PurposeContains the insert used for step 2 of edit-directed plasmid cloningDepositorInsertKanR
UseCRISPRTagsExpressionYeastMutationChanged sequence to remove a MluI cut sitePromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUB23-L-beta-gal
Plasmid#8820DepositorInsertbeta-gal
UseTagsUbiquitinExpressionYeastMutationL (leucine) at amino terminus of beta-galPromoterAvailable sinceDec. 28, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAV0543
Plasmid#133493PurposeExpression of nucleo-cytoplasmic mCherry in P-cellsDepositorInsertpLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046332
Plasmid#145413PurposeYeast Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV0479
Plasmid#133499PurposeExpression of sfGFP excluded from the nucleus with two NES signalsDepositorInsertpHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
EZ-L767
Plasmid#131773PurposePADH1_sFUS_Fusion Red_PixD_TACT1DepositorInsertPADH1_sFUS_Fusion Red_PixD_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-Kan
Plasmid#44948PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB4132
Plasmid#106127PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-SpHis5
Plasmid#44853PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZJOM108
Plasmid#133665PurposeIntegration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterpADH1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS12
Plasmid#139419PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.DepositorInsertSuperfolder GFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-600μΔ13V
Plasmid#28001DepositorInsertFLIPglu-600μΔ13V
UseTagseCFP and eYFPExpressionYeastMutationF16A in mglBPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pITI1
Plasmid#166094PurposeGal inducible expression of dCas9-FokIDepositorInsertdCas9-FokI
UseCRISPRTagsExpressionYeastMutationspCas9-D10A, H840APromoterGal-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pUDP003
Plasmid#101164PurposeE. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
LHP462 - Ub-HA
Plasmid#32169DepositorUseTagsHAExpressionYeastMutationPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVG48
Plasmid#164706PurposertTA under the control of PGK1 promoterDepositorInsertPGK1pr - rtTA - CYC1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2989
Plasmid#63636PurposepCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC625
Plasmid#62321PurposesgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellsDepositorInsertsgRNA +1x MS2, Pol II promoter with ribozyme cleavage
UseTagsExpressionYeastMutationPromoterpAdhAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDZ514 pURA MET25p PP7-PS-2x-yeGFP
Plasmid#72230PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXP822
Plasmid#46064DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGAL1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM159
Plasmid#84195PurposeDisturb the expression of miR159 in riceDepositorInsertmiR159
UseTagsExpressionBacterial, Plant, and Yeast…MutationPromoter2x35SAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3050(gRNA XII-5)
Plasmid#73292PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
YCPlac33-sGFP-VRG4
Plasmid#25447DepositorInsertSynthetic Green Fluorescent Protein - VRG4
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only