8,025 results
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Plasmid#107435PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET2 (MET2 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-EYFP
Plasmid#14220DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
p42Neo 3-F GW
Plasmid#58558PurposeMulticopy with G418 resistance marker for triple Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXRL2
Plasmid#63148PurposeLeu2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET3
Plasmid#107436PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET3 (MET3 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET17
Plasmid#107443PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET17 (MET17 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSK260
Plasmid#129252Purposedual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporterDepositorInsertSup35 NM yTRAP sensor reporting on mKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS314-hUD
Plasmid#11005DepositorInserthUD recombination system
UseTagsExpressionBacterial and YeastMutationDirect repeat recombination system. Recombination…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-HphMx
Plasmid#122895PurposeADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BiFC3
Plasmid#190048PurposeBiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)DepositorInsertYemVenus (ATG-aa173-238)
UseTagsExpressionYeastMutationPromoterMet3Available sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
BiFC4
Plasmid#190049PurposeBiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)DepositorInsertYemVenus (ATG-aa173-238)
UseTagsExpressionYeastMutationPromoterMet3Available sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
BiFC2
Plasmid#190047PurposeBiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)DepositorInsertYemVenus (aa1-172 - TAA)
UseTagsExpressionYeastMutationPromoterMet3Available sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
BiFC1
Plasmid#190046PurposeBiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)DepositorInsertYemVenus (aa1-172)
UseTagsExpressionYeastMutationPromoterMet3Available sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[3]-C (LEU2)
Plasmid#177796PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[3]ExpressionYeastMutationPromoterADH1Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-C (TRP1)
Plasmid#177795PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[1,2]ExpressionYeastMutationPromoterADH1Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCoofy42
Plasmid#55190PurposePichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTagsHis10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…ExpressionYeastMutationPromoterAOX1Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET14
Plasmid#107441PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET14 (MET14 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-mKO1-CaUra3
Plasmid#44638PurposeYeast tagging vector to create gene fusion with mKO1 with CaUra3 selectionDepositorInsertmKO1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBES2
Plasmid#83800PurposeUseful for tagging yeast proteins with N-terminal Venus tag; G418 selectionDepositorInsertVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
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PhyB-mCherry-SNF7
Plasmid#51574PurposeConstitutively express endosome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSNF7 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
E2-Crimson-FKBP(L)
Plasmid#85001PurposeExpresses E2-Crimson-FKBP(L)DepositorInsertE2-Crimson-FKBP(L)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-SweetTrac1
Plasmid#169083PurposeExpression of SweetTrac1 biosensor in yeastDepositorInsertSweetTrac1
UseTagsExpressionYeastMutationPromoterPMA1Available sinceFeb. 4, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pGBW-m4046574
Plasmid#148976PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAS2
Plasmid#12389DepositorInsertgreen fluorescent protein
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
GAL 103Q+ProCFPp303
Plasmid#15575DepositorInserthtt103Q + Pro (HTT Human)
UseTagsCFPExpressionYeastMutationProline-rich region presentPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-CHC1-msGFPx3
Plasmid#105256PurposePlasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.DepositorInsertChc1 portion (CHC1 Budding Yeast)
UseTagsmsGFPx3ExpressionYeastMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
YEplac181-TYS1
Plasmid#8629DepositorInsertTYS1 (TYS1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TKnatAX
Plasmid#73895Purposefission yeast drug counter-selectable selection markerDepositorInsertTK-nat
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMZ289
Plasmid#59898PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPS808
Plasmid#8856DepositorTypeEmpty backboneUseTagsGFPExpressionYeastMutationPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
YIp211-P30-His-POL30-3'
Plasmid#99546PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEmerald-Kan
Plasmid#44946PurposeYeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selectionDepositorInsertyoEmerald
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETcon(I-LtrI)
Plasmid#41523DepositorInsertI-LtrI
UseTags3x(G4S) Linker, G3S Linker, HA Tag, and Myc TagExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
GTH-y
Plasmid#81105PurposeGene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC593
Plasmid#62319PurposesgRNA with MS2-PP7 for yeast cellsDepositorInsertsgRNA + MS2-PP7 RNA binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP reporterExpressionYeastMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only