8,173 results
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Plasmid#162844PurposeYeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1DepositorInsert4_1_RNA-device
ExpressionYeastAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBP-4_1m.mutant
Plasmid#162869PurposeYeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1DepositorInsert4_1m_RNA-device
ExpressionYeastAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS5
Plasmid#83799PurposeUseful for tagging yeast proteins with N-terminal CFP tag; G418 selectionDepositorInsertCFP
ExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1
Plasmid#182512PurposeC-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.DepositorInsertCL1 Degron
TagsGFPExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-EGFP
Plasmid#14190DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS316 GAL cluster
Plasmid#61925PurposeExpression of GAL1 and GAL10 from S. cerevisiae cloned into pRS316DepositorAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)
Plasmid#52895PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]
TagsL-IFP-F[1]ExpressionYeastPromoterGAL1Available SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB-EGFP
Plasmid#14187DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJW1660
Plasmid#112034PurposeC-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEUDepositorInsertIAA17
TagsHAExpressionYeastMutationonly amino acids 71-114PromoterNoneAvailable SinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Cip-pAct1-Mnase
Plasmid#162556PurposeNheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.DepositorInsert3FLAG-Mnase-SV40
ExpressionYeastPromoterActineAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS10
Plasmid#83795PurposeUseful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selectionDepositorInsertCerulean
TagsCeruleanExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1-M7A
Plasmid#159167PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A
TagsSV40 NLSExpressionYeastMutationMutated Met 7 of the cyt b562 module of nuc-yHS1 …PromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFTK093
Plasmid#171365PurposeVector part (LVL1) from the Fungal Modular Cloning ToolKit.DepositorInsertsgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK056
Plasmid#171328PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertSpCas9-NLS
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6-mTFP1-His3MX6
Plasmid#69850PurposeC-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.DepositorInsertmTFP1
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-mDsRed-SpHis5
Plasmid#44639PurposeYeast tagging vector to create gene fusion with mDsRed with SpHis5 selectionDepositorInsertmDsRed
ExpressionYeastAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
LDB107
Plasmid#185423PurposeNUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporinDepositorInsertNUP1
TagsHAExpressionYeastAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyExpressionYeastPromoterAHD1Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPSG830
Plasmid#158601PurposetNCS-ePTS1 (2micron)DepositorInsertpTDH3-tNCS-ePTS1
TagsePTS1ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXP214
Plasmid#26829DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)
Plasmid#122067PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
TagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUB23-R-beta-gal
Plasmid#8818DepositorInsertbeta-gal
TagsUbiquitinExpressionYeastMutationR (arginine) at amino terminus of beta-galAvailable SinceDec. 28, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB-ECFP
Plasmid#14157DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJW1510
Plasmid#62364PurposepFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5DepositorInsertBirA-mVenus-Ubc6 tail anchor
ExpressionYeastMutationL232H mutation compared to standard mVenusPromoterSec63Available SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG415GAL-ccdB-EYFP
Plasmid#14217DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 K410N
Plasmid#87280PurposeEncodes Hsh155 K410N for expression in yeastDepositorAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEYC68-HA-TOR2 (pAS21)
Plasmid#39207DepositorAvailable SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETCON-HtsptLB12
Plasmid#45120DepositorInsertHtsptLB12
TagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SIK1
Plasmid#51577PurposeConstitutively express nucleolus localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsSIK1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ284
Plasmid#52225PurposeExpression of sgRNA precursor targeted against ade6+DepositorInsertrrk1-sgRNA
UseCRISPRExpressionYeastPromoterrrk1Available SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
PSUC2-yEGFPCLN2PEST-pRS406
Plasmid#64405PurposeSUC2 promoter driven GFP expressionDepositorInsertSUC2 promoter (SUC2 Budding Yeast)
TagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastPromoterSUC2Available SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTAL6-BB
Plasmid#36034DepositorTypeEmpty backboneExpressionYeastPromoterPTEF1Available SinceJuly 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG426Gal-GFP
Plasmid#38310DepositorInsertEGFP (eGFP )
ExpressionYeastAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I-T78H
Plasmid#100963PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFPDepositorInsertAmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
ExpressionYeastMutationF138I, T78HPromoterPMAAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFTK088
Plasmid#171360PurposeAMA1 sequence as Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertAMA1 sequence (short), entry vector providing fungal replication
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046464
Plasmid#148970PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpGAL1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJO818 (pRS425 Gal1-Ufd2-flag)
Plasmid#159522Purposeexpresses ufd2-flag in yeast cellsDepositorInsertUfd2
TagsFlagExpressionYeastPromoterGal1Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only