8,016 results

1. pAR-Ec633

   Plasmid

   #130873

   Purpose

   A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

   Depositor

   Chang Liu

   Article

   Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

   Insert

   TP-DNAP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   L477V, L640Y, I777K, W814N

   Promoter

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ampl43

   Plasmid

   #161830

   Purpose

   pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pV1200

   Plasmid

   #111429

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRG203MX

   Plasmid

   #64521

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PRS415-Gal1pr-sctetR-FokI

   Plasmid

   #44756

   Depositor

   Narendra Maheshri

   Article

   Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

   Insert

   sctetR-FokI (fokI Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1pr

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

   Plasmid

   #104394

   Purpose

   Yeast MCP-GFP expression

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   MCP-NLS-2xyeGFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   MCP fused to NLS(SV40) and 2x yeast codon optimiz…

   Promoter

   CYC1

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

   Plasmid

   #116884

   Purpose

   FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RLR)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFG4

   Plasmid

   #87251

   Purpose

   gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Hyg
   

   Use

   Tags

   parS segments

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LT1_36_QUEEN-2m

   Plasmid

   #177708

   Purpose

   Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   pTEF1-QUEEN2m
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lifeact-3xmNG::HIS3

    Plasmid

    #227596

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::HIS3
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. yHS1-M7A

    Plasmid

    #159151

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1-M7A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of yHS1 to A…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pRS305-Leu2-PINO4-PfV-GS-Sapphire

    Plasmid

    #116930

    Purpose

    Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    INO4

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pV1093:SpRY

    Plasmid

    #172188

    Purpose

    Genome editing of C. albicans

    Depositor

    Doug Bernstein

    Article

    Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. yHS1-M7A,H102A

    Plasmid

    #159152

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1-M7A,H102A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pFA6a-link-ymNeongreen-URA3

    Plasmid

    #168059

    Purpose

    Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeonGreen
    

    Use

    Tags

    ymNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SJZ5-HPH

    Plasmid

    #228258

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    hph
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSA1 S.c.

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCASB

    Plasmid

    #190175

    Purpose

    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

    Depositor

    Jessica Tyler

    Article

    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

    Insert

    sgRNA cloning site
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Added 5'-agagacc-3' upstream and 5'…

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGR481

    Plasmid

    #213783

    Purpose

    Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations

    Depositor

    Chang Liu

    Insert

    ura3*
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

    Plasmid

    #171104

    Purpose

    Expresses sgRNA (ENO1 gRNA) in Candida auris.

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNTI647 dCas9-Mxi1 TetR KanMX

    Plasmid

    #139474

    Purpose

    Integrates CRISPRi effector and tet-responsive regulator

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Inserts

    dCas9-Mxi1
    Tet Repressor
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1 and pTEF1

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTO135_pENO1-CaCas9-tCyc1

    Plasmid

    #171103

    Purpose

    Expresses Cas9 in Candida auris

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    CaCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS405-PTEF-Dcr1

    Plasmid

    #22314

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Sep 10. ():.

    Insert

    DCR1
    

    Use

    Integrating plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS404-PTEF-Ago1

    Plasmid

    #22313

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Sep 10. ():.

    Insert

    AGO1
    

    Use

    Integrating plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TurboID-V5_pRS415

    Plasmid

    #107167

    Purpose

    expresses V5-tagged TurboID in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYDS2.0_AT118-L

    Plasmid

    #222847

    Purpose

    Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LT2_7_OxPro

    Plasmid

    #177709

    Purpose

    Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTRX2_5xUAS-ymYPET-tCYC1
    pTEFmut8-mCherry-tADH1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFM689

    Plasmid

    #74635

    Purpose

    alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Inserts

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

    Plasmid

    #158585

    Purpose

    Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    membrane localization signal-tagBFP-wrmScarlet11
    

    Use

    Tags

    tagBFP

    Expression

    Yeast

    Mutation

    GGGS linker

    Promoter

    TEF

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pABY-T1

    Plasmid

    #201764

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMT899

    Plasmid

    #228288

    Purpose

    Constitutive mutant rPhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    rphlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge