8,033 results

1. pICE LYS2-HIS3

   Plasmid

   #122887

   Purpose

   LYS2-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICE LYS2-MET17

   Plasmid

   #122889

   Purpose

   LYS2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pICE TRP1-LEU2

   Plasmid

   #122892

   Purpose

   TRP1-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC119-Padh81-GBP-mCherry(N)-hphMX6-lys1*

   Plasmid

   #89072

   Purpose

   genomic GBP-mCherry epitope N-terminal tagging at lys1+ locus in S. pombe

   Depositor

   Quanwen Jin

   Article

   Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

   Type

   Empty backbone

   Use

   Genomic integration in s. pombe

   Tags

   GBP-mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPS2037

   Plasmid

   #1370

   Depositor

   Pamela Silver

   Article

   Brodsky et al RNA 2000 Dec;6(12):1737-49.

   Insert

   PGK1-U1A-PGK1 3' UTR (PGK1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pICE ADE2-LEU2

   Plasmid

   #122884

   Purpose

   ADE2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDRF’- AmTryoshka1;3-GS

   Plasmid

   #100967

   Purpose

   Yeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   AmTryoshka1;3-GS (AMT1;3 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PMA

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWS033

   Plasmid

   #123056

   Purpose

   Green fluorescent protein

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   sfGFP [3]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG413GAL-Cerulean-ccdB

   Plasmid

   #14405

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 68Q-ymsfGFP

    Plasmid

    #128560

    Purpose

    Httex1 (without proline-rich region) fused to ymsfGFP

    Depositor

    Patrick Lajoie

    Article

    Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

    Insert

    68Q-Httex1 (without proline-rich region) fused to ymsfGFP
    

    Use

    Tags

    ymsfGFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-kanMX6-P41nmt1-mEGFP

    Plasmid

    #105140

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

    Insert

    kanMX6-P41nmt1-mEGFP
    

    Use

    Tags

    41nmt1-mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    41nmt1

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS406-PHO5-GFP-Scs2

    Plasmid

    #58834

    Purpose

    Expresses GFP-Tagged Scs2 in yeast

    Depositor

    Tim Levine

    Article

    Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

    Insert

    Scs2 (SCS2 Budding Yeast)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pAG415GPD-Cerulean-ccdB

    Plasmid

    #14410

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-hygML

    Plasmid

    #73892

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    hygML
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFP-FKBP(M)x4

    Plasmid

    #85003

    Purpose

    Expresses EGFP-FKBP(M)x4 in yeast

    Depositor

    Benjamin Glick

    Article

    Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

    Insert

    EGFP-FKBP(M)x4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNIA-CEN-Venus-FLAG-LINXa4-Bre1

    Plasmid

    #73618

    Purpose

    Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    Venus
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGAD424-rSyntenin1

    Plasmid

    #89425

    Purpose

    Two-hybrid system

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP
    

    Use

    Tags

    Gal4 AD

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YIp211-P30-His-pol30(K127/164R)-3'

    Plasmid

    #99549

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R, K164R

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CUP Snf5 YFP p316

    Plasmid

    #15597

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    SNF5 (SNF5 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. LD214

    Plasmid

    #69185

    Purpose

    Integration of 2GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg14-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LD205

    Plasmid

    #69180

    Purpose

    Integration of 2GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg5-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS316-RGR-GFP-mHDV

    Plasmid

    #51058

    Purpose

    Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Yeast ADH1 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS316-RGR-GFP-mHH

    Plasmid

    #51057

    Purpose

    Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Yeast ADH1 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG414GAL-ccdB-EYFP

    Plasmid

    #14215

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPTK014-3a-Killer-αMFΔ

    Plasmid

    #84973

    Purpose

    Type 3a, Killer followed by αMFΔ - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Killer followed by αMFΔ secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pACT2 V1H (P#663)

    Plasmid

    #14665

    Depositor

    Matija Peterlin

    Article

    Geyer et al Mol Biol Cell. 2002 Jun . 13(6):2045-56.

    Insert

    V1H (ATP6V1H Human)
    

    Use

    Tags

    Gal4 AD

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pUDE714

    Plasmid

    #103021

    Purpose

    expression of a Cpf1 programming crRNA targetting HIS4 (crHIS4-4.S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crHIS4-4.S (HIS4 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPS2189

    Plasmid

    #1381

    Depositor

    Pamela Silver

    Article

    Hood et al J Cell Sci 2001 Feb;114(Pt 3):589-97.

    Insert

    Gal-Clb2-GFP (CLB2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

29. D1433 his3::LYS2 Disruptor Converter

    Plasmid

    #51676

    Purpose

    Yeast marker swap plasmid for converting HIS3 to LYS2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LYS2 (LYS2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pILGFP5V1

    Plasmid

    #165058

    Purpose

    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1- T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJAID8Z104

    Plasmid

    #165056

    Purpose

    Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGWKS2

    Plasmid

    #139410

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    GFP S65T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJK276

    Plasmid

    #73366

    Purpose

    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)

    Depositor

    T. J. Kappock

    Insert

    plastid omega-3 fatty acid desaturase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Mutation I231F. Also insert is missing residues 1…

    Promoter

    GAL1

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJK277

    Plasmid

    #71565

    Purpose

    Produces Crepis alpina delta15 fatty acid desaturase (FAD3) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta15 fatty acid desaturase
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAG424GAL-ccdB-EYFP

    Plasmid

    #14223

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAG416GAL-EYFP-ccdB

    Plasmid

    #14339

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAH235

    Plasmid

    #121145

    Purpose

    Cas9 expression controlled by the nmt41 promoter

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Insert

    humanized Streptococcus pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRS314-SNU114

    Plasmid

    #111309

    Purpose

    pRS314-SNU114

    Depositor

    Christine Guthrie

    Article

    Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

    Insert

    SNU114
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LHP308 - Ub - wt

    Plasmid

    #32151

    Depositor

    Linda Hicke

    Article

    Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pRS316-IVa

    Plasmid

    #31456

    Depositor

    Ana Kitazono

    Article

    Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

    Insert

    linker
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only