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Plasmid#58442PurposeCassette to replace yeast promoters with an inducible promoter containing 4 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
Plasmid#58440PurposeCassette to replace yeast promoters with an inducible promoter containing 2 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
Plasmid#58439PurposeCassette to replace yeast promoters with an inducible promoter containing 1 lexA boxDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
Plasmid#58441PurposeCassette to replace yeast promoters with an inducible promoter containing 3 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAC690-TEF
Plasmid#160432PurposeExpresses eGFP under control of the yeast TEF2 promoterDepositorInsertS. cerevisiae TEF2 promoter
UseTagsExpressionYeastMutationPromoterS. cerevisiae TEF2Available sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator
Plasmid#158585PurposeExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiaeDepositorInsertmembrane localization signal-tagBFP-wrmScarlet11
UseTagstagBFPExpressionYeastMutationGGGS linkerPromoterTEFAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastMutationPromoterTEF1pAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTEF2
Plasmid#43971DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterTEFAvailable sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pTEF6-7
Plasmid#124583PurposeReporter plasmid with constitutively expressed mCherry and YFP (eCitrine)DepositorInsertmCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS42H_PTEF1_dCas9_TCYC1
Plasmid#177155PurposeExpresses dCas9 in yeast cellsDepositorInsertNuclease-null Cas9
UseTagsSV40 NLSExpressionYeastMutationPromoterTEF1 promoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pHO_pTEFmut7_CggR_R250A_ble
Plasmid#124585PurposePlasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoterDepositorInsertcggR_R250A (cggR Synthetic)
UseSynthetic BiologyTagsExpressionMutationchanged arginine 250 to alaninePromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHO_pTEFmut7_CggR_ble
Plasmid#124584PurposePlasmid for genomic integration of CggR repressor regulated by the pTEFmut7 promoterDepositorInsertcggR (cggR Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p414TEF_Hsp82_CycTER
Plasmid#61711PurposeYeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterTEF1Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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BB1_12_YlpTEF
Plasmid#117819PurposePromoter pTEF of Y. lipolytica for GoldenMOCSDepositorInsertYlpTEF
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEF1_MS2
Plasmid#220625PurposeSource for TEF1 MS2 coat fusion protein fusionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pTEF2
Plasmid#98507PurposeBB1 with insert: promoter PTEF2DepositorInsertTEF2 promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p414TEF_Hsp82_noTER
Plasmid#61714Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterTEF1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
LT1_33_pTEFmut8-mCherry
Plasmid#177706PurposeConstruct for genome integration expressing constitutively mCherry under pTEFmut8 promoterDepositorInsertpTEFmut8-mCherry
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPL5608_TEF1*-Cre_TRP1
Plasmid#60931PurposeConstitutive expression vector for Cre-recombinase containing TRP1 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromoterMutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPL5606_TEF1*-Cre_ADE2
Plasmid#60932PurposeConstitutive expression vector for Cre-recombinase containing ADE2 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPL5071_TEF1*-Cre_URA3
Plasmid#60930PurposeConstitutive expression vector for Cre-recombinase containing URA3 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p426TEF-LptTPS-A
Plasmid#89468PurposeExpression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene productionDepositorInsertLptTPS-A
UseTagsnoneExpressionYeastMutationPromoterpBR322 origin of replication for propagation in E…Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-Sir2
Plasmid#51742Purposeoverexpression of Sir2 in S. cerevisiae, HIS3 markerDepositorInsertSir2 (SIR2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only