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We narrowed to 255 results for: px459

Showing: 1 - 30 of 255 results

  1. pX459-sgAAVS1

    Plasmid
    #184403
    Purpose
    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    AAVS1-specific sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184403-plasmid-map-sequence-id-362381

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  8. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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  22. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  23. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Article
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    118632-plasmid-map-sequence-id-230217

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  30. pX459_MCM2_sgRNA

    Plasmid
    #232730
    Purpose
    Generates MCM2 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    MCM2 (MCM2 Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Promoter
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232730-plasmid-map-sequence-id-465451

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Showing: 1 - 30 of 255 results