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Plasmid#173080PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGAP
UseTagsmCitrineExpressionBacterialMutationmCitrine + last 10 residues of GNA1, codon optimi…PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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GI-Gal4 DBD
Plasmid#42500PurposeGIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factorDepositorInsertGIGANTEA fused with Gal4 DBD
UseTagsGal4 DNA binding domain (DBD)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GI-ZFP2
Plasmid#42215PurposeExpresses GI-ZFP2 for light-inducible activation of gene expressionDepositorInsertGIGANTEA-FLAG-ZFP2 (GI Mustard Weed, Synthetic)
UseTagsFLAGExpressionMutationPromoterCMVAvailable sinceJan. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFVp-gI-BlastR
Plasmid#207179PurposeGateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVpAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SV40p-gI-BlastR
Plasmid#207182PurposeGateway compatible Lentiviral Expression Plasmid SV40p-IRES-BlastRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM4D(Gi)-mCherry
Plasmid#50475PurposeGi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-hM4D(Gi)-mCherry
Plasmid#50479PurposeGi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoterDepositorHas ServiceAAV5InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Plasmid#50477PurposeGi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV CD68-hM4D(Gi)-mCherry
Plasmid#75033PurposeExpression of hM4D(Gi) using the CD68 promoter.DepositorHas ServiceAAV1 and AAV9InserthM4D(Gi)-mCherry
UseAAVTagsExpressionMammalianMutationPromoterCD68Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM4D(Gi)
Plasmid#45548PurposeExpresses hM4D (Gi) neuronal inhibitorDepositorInserthM4D (Gi)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAM-FLEX-hM4D(Gi)-mCherry
Plasmid#232782PurposeAAV2 vector containing Gi-coupled hM4D DREADD fused with mCherry inside a double floxed (FLEX) cassette under the control of CBA promoter.DepositorInserthM4D(Gi)-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertWilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135812PurposeThis plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertTagRFP
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM4D(Gi)-mCherry
Plasmid#50461PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Plasmid#44362PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry
UseAAV; Adeno associated viral vectorTagsmCherryExpressionMutationPromoterhuman Synapsin 1Available sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1329 - pAAV EF1a HA-hM4D(Gi)
Plasmid#89150PurposeAn AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.DepositorInserthM4D(Gi)
UseAAVTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-GFP(LaG16) PAGER(Gi)
Plasmid#230008PurposeExpresses anti-GFP PAGER(Gi) in mammalian cellsDepositorInsertMT1-LaG16-TEVcs-ALFA-hM4D
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-anti-mCherry(LaM6) PAGER(Gi)
Plasmid#230018PurposeLentiviral vector expressing anti-mCherry PAGER(Gi) in mammalian cellsDepositorInsertMT1-LaM6-TEVcs-ALFA-hM4D
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-mCherry(LaM6) PAGER(Gi)
Plasmid#230012PurposeExpresses anti-mCherry PAGER(Gi) in mammalian cellsDepositorInsertMT1-LaM6-TEVcs-ALFA-hM4D
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)
Plasmid#121538PurposeAn adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoterDepositorUseAAVTagsHAExpressionMammalianMutationPromoterSYN1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-DIO-hM4D(Gi)-mCherry
Plasmid#190240PurposeExpresses iDREADD fused to mCherry flanked by DIODepositorInserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationPromotermouse CamKII alphaAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon DREADD Gi-mCherry
Plasmid#177669PurposeExpresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cellsDepositorInsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon DREADD Gi-mCherry
Plasmid#177672PurposeExpresses DREADD hM4D (Gi) in Cre and Flp expressing cellsDepositorHas ServiceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA
Plasmid#154867PurposeFlp-dependent hM4D(Gi) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM4D(Gi)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gi)
Plasmid#230019PurposeAAV vector expressing anti-mCherry PAGER(Gi) in neuronsDepositorInsertMT1-LaM6-TEVcs-ALFA-hM1D
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SFFV-anti-mCherry(LaM6) PAGER(Gi)
Plasmid#230021PurposeLentiviral vector expressing anti-mCherry PAGER(Gi) in human T cellsDepositorInsertMT1-LaM6-TEVcs-ALFA-hM4D
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff DREADD Gi-mCherry
Plasmid#177673PurposeExpresses DREADD hM4D (Gi) only in Cre expressing cells and not in Flp expressing cellsDepositorHas ServiceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)
Plasmid#125146PurposeAn adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoterDepositorInsertHA-tagged hM4D(Gi) (CHRM4 Human)
UseAAV and Cre/LoxTagsHAExpressionMammalianMutationPromoterSYN1Available sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50464PurposeGi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoteDepositorInserthM4D(Gi)-IRES-mCitrine (CHRM4 Human)
UseAAVTagsHAExpressionMutationPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50471PurposeGi-coupled hM4D-IRES-mCitrine under the control of GFAP promoterDepositorInserthM4D(Gi)-IRES-mCitrine (CHRM4 Human)
UseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-con/fon DREADD Gi-mCherry
Plasmid#200660PurposeConstruct for chemogenetically silencing and visualizing subpopulations of neurons in the retinaDepositorInsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50467PurposeGi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-frt-DIO-hM4D(Gi)-mCherry
Plasmid#217398PurposeExpresses the hM4D(Gi) inhibitory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoterDepositorInserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationPromoterhuman Synapsin 1Available sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50455PurposeDouble floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1
Plasmid#183399PurposeDual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)
Plasmid#183704PurposeAAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limitDepositorInserthM4D(Gi)
UseAAVTagsmCherryExpressionMutationPromoterCaMKIIaAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1
Plasmid#183402PurposeDual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1
Plasmid#183400PurposeDual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only