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Showing: 451 - 500 of 12598 results

  2. mScarlet-I-LSS-SGFP2

    Plasmid
    #112945
    Purpose
    Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET
    Depositor
    Dorus Gadella
    Insert
    LSS-GFP2 mScarlet-I Fusion
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112945-plasmid-map-sequence-id-223563

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  3. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

    Plasmid
    #112288
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    3 Tyrosines (341, 357 and 394 corresponding to ty…
    Promoter
    EF-1 alpha
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112288-plasmid-map-sequence-id-213531

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  4. pJUNB-donor

    Plasmid
    #112313
    Purpose
    CRISPR donor plasmid to tag human transcription factor JUNB with GFP
    Depositor
    Kevin White
    Insert
    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    Use
    CRISPR
    Mutation
    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112313-plasmid-map-sequence-id-228468

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  6. pOP1

    Plasmid
    #112595
    Purpose
    Used for expression of protein in E. coli as non-fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112595-plasmid-map-sequence-id-220200

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  7. RhoA sensor (LSS-SGFP2-Scarlet-I)

    Plasmid
    #112947
    Purpose
    RhoA FRET biosensor
    Depositor
    Dorus Gadella
    Insert
    RhoA sensor
    Tags
    LSS-SGFP2 and mScarlet-I
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112947-plasmid-map-sequence-id-220211

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  9. LUC56 (Luc/UGA/SP)

    Plasmid
    #112147
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Rat, Photinus pyralis)
    Expression
    Mammalian
    Mutation
    Cysteine 258mutated to selenocysteine (U)
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112147-plasmid-map-sequence-id-214894

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  10. pHEY1.1.0-gDNA

    Plasmid
    #112393
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1
    Depositor
    Kevin White
    Insert
    HEY1 (HEY1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112393-plasmid-map-sequence-id-230239

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  11. pHEY1-donor

    Plasmid
    #112297
    Purpose
    CRISPR donor plasmid to tag human transcription factor HEY1 with GFP
    Depositor
    Kevin White
    Insert
    HEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
    Use
    CRISPR
    Mutation
    hg38:8:79765311:G>A
    Available Since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    112297-plasmid-map-sequence-id-225585

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  12. pcDNA3.1-hRIPK1-Flag-K377R

    Plasmid
    #112489
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K377R
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112489-plasmid-map-sequence-id-214536

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  13. pSNAI2-donor

    Plasmid
    #112378
    Purpose
    CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP
    Depositor
    Kevin White
    Insert
    SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)
    Use
    CRISPR
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112378-plasmid-map-sequence-id-229744

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  15. pVHC

    Plasmid
    #112614
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112614-plasmid-map-sequence-id-224545

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  18. rd-his

    Plasmid
    #112911
    Purpose
    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag
    Depositor
    C. James McKnight
    Article
    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20
    Insert
    dematin (DMTN Human)
    Tags
    6-his tag, Glutathione-S-Transferase, and preciss…
    Expression
    Bacterial
    Mutation
    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…
    Promoter
    T7
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112911-plasmid-map-sequence-id-220214

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  25. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid
    #112029
    Purpose
    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    Tags
    Split mCherry
    Expression
    Bacterial
    Available Since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112029-plasmid-map-sequence-id-213508

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  29. pTEAD1-donor

    Plasmid
    #112329
    Purpose
    CRISPR donor plasmid to tag human transcription factor TEAD1 with GFP
    Depositor
    Kevin White
    Insert
    TEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
    Use
    CRISPR
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112329-plasmid-map-sequence-id-228556

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  38. mCherry-T2A-LSS-SGFP2

    Plasmid
    #112944
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Insert
    mCherry and LSS-SGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112944-plasmid-map-sequence-id-220241

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  39. pZBTB9.1.0-gDNA

    Plasmid
    #112469
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB9
    Depositor
    Kevin White
    Insert
    ZBTB9 (ZBTB9 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112469-plasmid-map-sequence-id-230499

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  40. pZBTB5.1.0-gDNA

    Plasmid
    #112399
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB5
    Depositor
    Kevin White
    Insert
    ZBTB5 (ZBTB5 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112399-plasmid-map-sequence-id-230250

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  41. pZBTB9-donor

    Plasmid
    #112373
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB9 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB9 homology arms flanking EGFP-IRES-Neo cassette (ZBTB9 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112373-plasmid-map-sequence-id-229732

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  42. pZBTB5-donor

    Plasmid
    #112303
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB5 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)
    Use
    CRISPR
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112303-plasmid-map-sequence-id-228415

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  46. pDLX1-donor

    Plasmid
    #112343
    Purpose
    CRISPR donor plasmid to tag human transcription factor DLX1 with GFP
    Depositor
    Kevin White
    Insert
    DLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
    Use
    CRISPR
    Mutation
    hg38:2:172087403:G>C,hg38:2:172087433:G>A,h…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112343-plasmid-map-sequence-id-229657

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  49. pOP2H

    Plasmid
    #112596
    Purpose
    Used for expression of protein in E. coli as non-cleavable C-terminal 8His-tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112596-plasmid-map-sequence-id-220202

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Showing: 451 - 500 of 12598 results