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Plasmid#226665PurposePiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidinDepositorInsertprotective nicking sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Parkin(C431N)-A92mKO2
Plasmid#213545PurposeTransient mammalian expresssion of ligase-dead C431N Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseTagsExpressionMammalianMutationC431N mutation in ParkinPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PIP4K2A-Kina
Plasmid#202745Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertmCherry:FKBP1A(3-108) : [GGSA]4GG :PIP4K2A (33-406) (PIP4K2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO::EGFP-hMeikin(8A mut)
Plasmid#174707PurposeDoxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 bindingDepositorInsertMeikin
UseTagsEGFPExpressionMammalianMutationS175A, T176A, T180A, S181A, S196A, T251A, T264A, …PromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/666E
Plasmid#105313PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/666EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/666A
Plasmid#105309PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/666APromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
TIGRE docking site targeting vector
Plasmid#61580PurposeCreate a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchangeDepositorTypeEmpty backboneUseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-human-p16INK4A-P2A-mCherry
Plasmid#137026Purposedonor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4ADepositorInsertp16INK4A-knockin-homology arm-P2A-mCherry
UseTagsP2A-mCherryExpressionBacterialMutationPromoterno promoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMN_HA-TBK1 (kinase dead - D135N)
Plasmid#199206PurposeUsed for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)DepositorInsertTBK1 (TBK1 Human)
UseRetroviralTagsExpressionMammalianMutationD135NPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
H2B-mScarlet- hMeikin(1-332)-mNeonGreen
Plasmid#174714PurposeStable expression of fluorescent Separase cleavage sensor with human MeikinDepositorInsertMeikin
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation1-332PromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
K84M kinase-dead Nuak1-3xflag
Plasmid#97219PurposePlasmid expressing kinase-dead mutant Nuak1DepositorInsertNuak1 (NUAK1 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationKinase-dead K84MPromoterT7Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hela kinase-dead MLCK-GFP
Plasmid#46317DepositorInsertHeLa long myosin light chain kinase-dead (MYLK Human)
UseTagsGFPExpressionBacterial and MammalianMutationE1626KPromoterCMVAvailable sinceJuly 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFUChW Parkin shRNA Mul1 shRNA
Plasmid#78523PurposeLentiviral vector encoding two shRNAs and mCherry reporterDepositorUseLentiviral and RNAiTagsExpressionMutationPromoterH1Available sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#119186PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I no force control (F40-based)
Plasmid#119187PurposeThe no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.DepositorInserthuman Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationtruncation after aa1945PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II tension sensor (F40-based)
Plasmid#118715PurposeThe F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag
Plasmid#185682PurposeLow-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
UseTagsHaloTagExpressionMammalianMutationK599APromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human Inositol 1,4,5-Trisphosphate 3-Kinase B
Plasmid#122589PurposeExpression of human inositol 1,4,5-trisphosphate 3-kinase BDepositorInsertinositol 1,4,5-trisphosphate 3-kinase B (ITPKB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc
Plasmid#161586Purposeexpression of Myc-tagged mouse Lrrk2 without kinase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1871-2540PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIG-738pUC19-tNGFR-P2A-Kindlin-3
Plasmid#186092PurposeKnockin of truncated NGFR to target geneDepositorInsertKINDLIN3 (FERMT3 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only